AtHsfA2 modulates expression of stress responsive genes and enhances tolerance to heat and oxidative stress in Arabidopsis

  • Li Chunguang 
  • Qijun Chen
  • Xinqi Gao
  • Bishu Qi
  • Naizhi Chen
  • Shouming Xu
  • Jia Chen
  • Xuechen Wang
Article
  • 99 Downloads

Abstract

There is increasing evidence for considerable interlinking between the responses to heat stress and oxidative stress, and recent researches suggest heat shock transcription factors (Hsfs) play an important role in linking heat shock with oxidative stress signals. In this paper, we present evidence that AtHsfA2 modulated expression of stress responsive genes and enhanced tolerance to heat and oxidative stress in Arabidopsis. Using Northern blot and quantitative RT-PCR analysis, we demonstrated that the expression of AtHsfA2 was induced by not only HS but also oxidative stress. By functional analysis of AtHsfA2 knockout mutants and AtHsfA2 overexpressing transgenic plants, we also demonstrated that the mutants displayed reduced the basal and acquired thermotolerance as well as oxidative stress tolerance but the overexpression lines displayed increased tolerance to these stress. The phenotypes correlated with the expression of some Hsps and APX1, ion leakage, H2O2 level and degree of oxidative injuries. These results showed that, by modulated expression of stress responsive genes, AtHsfA2 enhanced tolerance to heat and oxidative stress in Arabidopsis. So we suggest that AtHsfA2 plays an important role in linking heat shock with oxidative stress signals.

Keywords

heat shock transcription factor heat stress response oxidative stress response tolerance 

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Copyright information

© Science in China Press 2005

Authors and Affiliations

  • Li Chunguang 
    • 1
    • 2
  • Qijun Chen
    • 1
  • Xinqi Gao
    • 1
    • 3
  • Bishu Qi
    • 1
  • Naizhi Chen
    • 1
  • Shouming Xu
    • 1
  • Jia Chen
    • 1
  • Xuechen Wang
    • 1
  1. 1.State Key Laboratory of Plant Physiology and Biochemistry, College of Biological SciencesChina Agricultural UniversityBeijingChina
  2. 2.Department of Industry EngineeringZhengzhou Institute of Aeronautic Industry ManagementZhengzhouChina
  3. 3.College of Life ScienceQufu Normal UniversityQufu ShandongChina

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