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Science in China Series B: Chemistry

, Volume 48, Issue 2, pp 162–166 | Cite as

Studies of poly-L-lysine-starch nanoparticle preparation and its application as gene carrier

  • Suyao Xiao
  • Xuanming Liu
  • Chunyi Tong
  • Jun Liu
  • Dongying Tang
  • Lijian Zhao
Article
  • 150 Downloads

Abstract

Anion starch nanoparticle (StNP) with a diameter of 50 nm was prepared in water-in-oil microemulsion, with soluble starch as raw materials and POCl3 as crosslinking agent. PLL-StNP was prepared by linking poly-L-lysine (PLL) on the surface of StNP. At the same time, the size of PLL-StNP and its stability in aqueous solution were checked by AFM. The analysis of plasmid DNA binding, DNase I enzymatic degradation, toxicity and transfection were done. We discovered that PLL-StNP may be used as non-virus nanoparticle gene carrier. And we developed the method of preparing PLL-StNP gene carrier and used it in cell transfection. As non-virus gene carrier, PLL-StNP has some advantages, such as large load of DNA, high transfection efficiency, low cell toxicity and biodegradability.

Keywords

anion starch nanoparticle poly-L-lysine non-virus gene carrier cell transfection 

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Copyright information

© Science in China Press 2005

Authors and Affiliations

  • Suyao Xiao
    • 1
  • Xuanming Liu
    • 1
  • Chunyi Tong
    • 1
  • Jun Liu
    • 1
  • Dongying Tang
    • 1
  • Lijian Zhao
    • 1
  1. 1.Institute of Life Science and Biotechnology, College of Chemistry & Chemical EngineeringHunan UniversityChangshaChina

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