Abstract
Anion starch nanoparticle (StNP) with a diameter of 50 nm was prepared in water-in-oil microemulsion, with soluble starch as raw materials and POCl3 as crosslinking agent. PLL-StNP was prepared by linking poly-L-lysine (PLL) on the surface of StNP. At the same time, the size of PLL-StNP and its stability in aqueous solution were checked by AFM. The analysis of plasmid DNA binding, DNase I enzymatic degradation, toxicity and transfection were done. We discovered that PLL-StNP may be used as non-virus nanoparticle gene carrier. And we developed the method of preparing PLL-StNP gene carrier and used it in cell transfection. As non-virus gene carrier, PLL-StNP has some advantages, such as large load of DNA, high transfection efficiency, low cell toxicity and biodegradability.
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Xiao, S., Liu, X., Tong, C. et al. Studies of poly-L-lysine-starch nanoparticle preparation and its application as gene carrier. Sc. China Ser. B-Chem. 48, 162–166 (2005). https://doi.org/10.1360/04yb0010
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DOI: https://doi.org/10.1360/04yb0010