Chinese Science Bulletin

, Volume 49, Issue 19, pp 2053–2057 | Cite as

Expression of lysine-rich protein gene and analysis of lysine content in transgenic wheat

  • Chaomin Meng
  • Xüqing Chen
  • Rongqi Liang
  • Fengping Yang
  • Liquan Zhang
  • Xiaodong Zhang
  • Tianyou Chen
  • S. S. M. Sun
Articles
  • 72 Downloads

Abstract

Expression vector pBPC102, which carries winged bean lysine-rich protein (wblrp) gene and dihydropicolinate synthase (DHDPS) gene, was transferred into hexaploid winter wheat cv. Jinghua No.1, Jing411, You899 and Yangnong15 expiants of immature inflorescence and immature embryos by particle bombardment. More than 100 transgenic plants were obtained under the selection of s-(2-aminoethyl)-L-cysteine (AEC). Confirmed transgenic plants of T0 and T1 generation by PCR and PCR-Southern blotting analyses showed successful integration of wblrp gene into wheat genome. Analysis of transgenic plant lines of T2 by Northern dot-blotting showed good expression of wblrp gene in offspring seed. The content of free lysine in leaves, contents of bound lysine and total proteins in seeds of T2 transgenic wheat lines were determined and analyzed. Among 34 tested transgenic lines, levels of free lysine content in leaves of 9 transgenic lines are 2–3times higher than un-transformed wild-type cultivars. Among 17 analyzed transgenic lines, bound lysine content of 4 transgenic lines is more than 10% higher than that of wild-type cultivars. Our research suggests that introducing wblrp gene into wheat is an effective way to improve its nutrition quality.

Keywords

wheat (Triticum aestivum L.) lysine-rich protein transgenic expression nutrition quality 

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Copyright information

© Science in China Press 2004

Authors and Affiliations

  • Chaomin Meng
    • 1
    • 2
  • Xüqing Chen
    • 1
  • Rongqi Liang
    • 1
  • Fengping Yang
    • 1
  • Liquan Zhang
    • 1
  • Xiaodong Zhang
    • 1
  • Tianyou Chen
    • 2
  • S. S. M. Sun
    • 3
  1. 1.Beijing Agro-Biotechnology Research CenterBeijingChina
  2. 2.College of AgronomyNorthwest Sci-Tech University of Agriculture and ForestryYanglingChina
  3. 3.Department of BiologyThe Chinese University of Hong KongHong KongChina

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