The RFA regulatory sequence-binding protein in the promoter of prostate-specific antigen gene

  • Weiwen Chen
  • Jianye Zhang
  • Charles Y. F. Young
  • Lianying Zhang
  • Liucun Chen
  • Jian Zhao


To assure what sequence associated with the androgen regulation, a 15 bp region at the upstream of the ARE of prostate-specific antigen (PSA) promoter, termed RFA, was found indispensable for androgen receptor (AR)-mediated transactivation of PSA promoter. In transfection and CAT assays, some nucleotides substitution in RFA could significantly decrease the androgen inducibility for PSA promoter. The in vitro DNA binding assay demonstrated that RFA bound specifically with some non-receptor protein factors in prostate cell nucleus, but the mutant type of RFA lost this ability, so RFA might be a novel accessory cis-element. The RFA-binding proteins were isolated and purified by affinity chromatography using RFA probes. SDS-PAGE and preliminary protein identification showed these proteins possessed sequence high homology with multifunctional protein heterogeneous nuclear ribonucleoprotein A1, A2 (hnRNP A1, A2). RFA-binding proteins possibly cooperate with AR-mediated transactivation for PSA promoter as coactivator. The study results will facilitate further understanding the mechanism and tissue specificity of PSA promoter.


prostate-specific antigen promoter heterogeneous nuclear ribonucleoprotein A1 A2 


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Copyright information

© Science in China Press 2003

Authors and Affiliations

  • Weiwen Chen
    • 1
  • Jianye Zhang
    • 1
  • Charles Y. F. Young
    • 2
  • Lianying Zhang
    • 1
  • Liucun Chen
    • 1
  • Jian Zhao
    • 3
  1. 1.Institute of Biochemistry and Molecular Biology, School of MedicineShandong UniversityJinanChina
  2. 2.Department of Urology Research of Mayo Clinic/FoundationUSA
  3. 3.Department of Thoracic SurgeryQilu Hospital of Shandong UniversityJinanChina

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