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Microbial dehalogenation of trichlorophenol by a bacterial consortium: characterization and mechanism

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Abstract

Chlorinated phenolic compounds are a class of toxic and refractory organic pollutants. The pollution caused by chlorophenols poses serious ecological and environmental problems. A stable bacterial consortium capable of reductively dechlorinating trichlorophenol was isolated using chlorophenol as the sole source of carbon and energy. The physiological characteristics of the mixed cultures were studied and the results show that the consortium could use pyruvate as the carbon and energy source. The fermentation of pyruvate, sulfate reduction and dechlohnation process proceeded strictly in succession within this consortium. The effect of specific inhibitors on the dechlorinating activity of the consortium was investigated, and the results indicate that sulfate and molybdate (1 mmol/L) have a strong inhibitive influence on the dechlohnation activity. Fluorescence in situ hybridization (FISH) technique was applied to analyzing the composition of the consortium and the results reveal that one major subpopulation within the consortium was phylogenetically affiliated to gamma and delta subclass of Proteobacteria.

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Authors and Affiliations

  1. Laboratory of Environmental Technology, Institute of Nuclear Energy Technology, Tsinghua University, 100084, Beijing, China

    Wang Jianlong

  2. State Key Joint Laboratory of Environment Simulation and Pollution Control, Tsinghua University, 100084, Beijing, China

    Wang Jianlong

  3. Institut fuer Technische Umweltschutz, Technische Universitaet Berlin, Sekr KF 7, Strasse des 17, Juni 135, D-16023, Berlin, Germany

    Werner Hegemann

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  1. Wang Jianlong
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  2. Werner Hegemann
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Correspondence to Wang Jianlong.

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Jianlong, W., Hegemann, W. Microbial dehalogenation of trichlorophenol by a bacterial consortium: characterization and mechanism. Sc. China Ser. B-Chem. 46, 207–215 (2003). https://doi.org/10.1360/03yb9029

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  • DOI: https://doi.org/10.1360/03yb9029

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