Advertisement

Science in China Series B: Chemistry

, Volume 47, Issue 4, pp 267–275 | Cite as

A thin-layer spectroelectrochemical study of 3,3′,5,5′-tetramethylbenzidine at SnO2:F film optically transparent electrode

  • Jiao Kui 
  • Yang Tao 
  • Niu Shuyan 
Article
  • 81 Downloads

Abstract

The electrooxidation of 3,3′, 5,5′-tetramethylbenzidine (TMB) is dependent on the pH value of Britton-Robinson (B-R) buffer solution. In this work, the electrooxidation behavior of TMB was investigated with a SnO2:F film optically transparent thin-layer spectroelectrochemical cell. TMB underwent one two-electron electrooxidation process in the pH range from 2.0 to < 4.0, and two successive one-electron electrooxidation processes in the pH range from 4.0 to < 7.0 in the B-R buffer solution. At pH 6.5, the electrooxidative product of TMB generated a subsequent chemical reaction to yield an azo compound. Several spectroelectrochemical techniques, such as thin-layer cyclic voltammetry, thin-layer cyclic voltabsorptometry, thin-layer potential-controlled electrolysis absorptometry, thin-layer single-potential-step chronoabsorptometry, thin-layer double-potential-step chronoabsorptometry, thin-layer single-potential-step open-circuit relaxation chronoabsorptometry, were applied to this investigation. The formal potential E0′ and the electron transfer number corresponding to the electrooxidation of TMB in B-R buffer solution, and the reaction rate constant of the subsequent chemical reaction were determined.

Keywords

3,3′,5,5′-tetramethylbenzidine tin oxide film optically transparent electrode thin-layer spectroelectro-chemistry electrooxidation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Wang Tong, Sun Wei, Jiao Kui, Studies on the voltammetric enzyme-linked immunoassay system based on 3,3′,5,5′-tetrame-thylbenzidine (TMB) as substrate, Chinese Journal of Analytical Chemistry (in Chinese), 2002, 30(11): 1298–1302.Google Scholar
  2. 2.
    He Yanan, Chen Hongyuan, 3,3′,5,5′-Tetramethylbenzidine (TMB)-H2O2-HRP voltammetric immunoassay for the determination of IgE in human serum, Chem. J. Chinese Universities (in Chinese), 1997, 18(8): 1306–1308.Google Scholar
  3. 3.
    Jiao Kui, Zhang Shusheng, Sun Wei, Authorization Certificate of Science and Technology of Shandong Province, 1999, No: 197.Google Scholar
  4. 4.
    Jiao Kui, Lu Gang, Yang Tao et al., Electrochemical and spectroelectrochemical study of m-phenylenediamine, Chem. J. Chinese Universities (in Chinese), 2003, 24(6): 1005–1008.Google Scholar
  5. 5.
    Mohr, P., Harke, T., Kuhn, W. et al., Mechanism studies of enzymatically formed tolidine blue and determination of peroxidatic activities, Biomed. Biochim. Acta, 1983, 42(6): 663–672.Google Scholar
  6. 6.
    Matrka, M., Pipalova, J., Semiquinone formation during the oxidation of benzidine, o-tolidine, and o-dianisidine, Chem. Prum., 1971, 21(1): 14–18.Google Scholar
  7. 7.
    Shen Baoen, Wang Guiliang, Thin-layer spectroelectrochemical investigation on indigo carmine, Acta Physico-Chimica Sinica (in Chinese), 1990, 6(1): 71–76.Google Scholar
  8. 8.
    Jiao Kui, Zhang Shusheng, Wei Lu et al., Investigation of voltammetric enzyme-linked immunoassay system based on new system of OT- H2O2-HRP, Acta Chimica Sinica (in Chinese), 1997, 55: 1121–1129.Google Scholar
  9. 9.
    Wang Huawei, Shen Baoen, Peng Tuzhi et al., The mechanism of electrooxidation of o-tolidine, J. of Hangzhou Universities (in Chinese), 1994, 21(2): 198–203.Google Scholar
  10. 10.
    Cheng, G, Yang, Y, Dong, S., Spectroelectrochemical study of biliverdin produced by the electrooxidation of bilirubin. Bioelectrochem. Bioenerg., 1991, 26: 35–39.CrossRefGoogle Scholar
  11. 11.
    Dong Shaojun, Song Shihua, Cheng Guangjin, Optically thin-layer spectroelectrochemical investigation on murexide electroreduction progress, Acta Physico-Chimica Sinica (in Chinese), 1987, 3(4): 368–373.Google Scholar

Copyright information

© Science in China Press 2004

Authors and Affiliations

  1. 1.College of Chemistry and Molecular EngineeringQingdao University of Science and TechnologyQingdaoChina
  2. 2.College of Chemistry and Chemical EngineeringOcean University of ChinaQingdaoChina

Personalised recommendations