Science in China Series B: Chemistry

, Volume 47, Issue 2, pp 166–172 | Cite as

Applications of homemade kit in mutation detection of genes

  • Chunxia Zhao
  • Guowang Xu
  • Xianzhe Shi
  • Jianmei Ma
  • Yan Zhang
  • Shen Lü
  • Qing Yang
Article
  • 16 Downloads

Abstract

Several methods of mutation detection, such as single-strand conformation polymorphism (SSCP), tandem SSCP/heteroduplex analysis and SNaPshot analysis were developed using homemade kit on AB1 310 genetic analyzer, and were successfully applied to mutation detection of 31 colorectal tumor samples. The sieving capability of homemade kit and commercial kit were compared, results demonstrate that homemade kit has higher resolution and shorter analysis time. In clinical tumor samples, 26% K-ras (exon 1) and 24% p53 (exons 7–8) were found to have mutations, and all mutations were single point variations. A majority of mutations occurred in one gene, only 1 tumor contained alterations in the two genes, which indicates that development of colorectal cancer lies on alternate pathways, and may correlate with different gene mutations

Keywords

single nucleotide polymorphism (SNP) single-strand conformation polymorphism (SSCP) heteroduplex analysis (HA) SNaPshot linear polyacrylamide (LPA) polydimethylacrylamide (PDMA) 

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Copyright information

© Science in China Press 2004

Authors and Affiliations

  • Chunxia Zhao
    • 1
  • Guowang Xu
    • 1
  • Xianzhe Shi
    • 1
  • Jianmei Ma
    • 2
  • Yan Zhang
    • 2
  • Shen Lü
    • 1
    • 2
  • Qing Yang
    • 1
  1. 1.Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalianChina
  2. 2.Cell Laboratory, Second Affiliated HospitalDalian Medical UniversityDalianChina

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