Chinese Science Bulletin

, 48:549 | Cite as

Somatic cell bovine cloning: Effect of donor cell and recipients

  • Dayuan Chen
  • Jinsong Li
  • Zhiming Han
  • Lei Lei
  • Zhonghua Liu
  • Zhaohui Kou
  • Shiyuan Ma
  • Qike Du
  • Qingyuan Sun


Adult somatic cell nuclear transfer was conducted by using cultured ear fibroblast cells obtained from a Holstein female cow (GN) and a Galoway herd bull (GLV). The percentages of reconstructed eggs developed into blastocysts were similar in GN (23.98%, 123 of 513) and in GLV groups (29.55%, 138 of 467). However, the rate of reconstructed female (GN) embryos developed into term was higher than that of male (GLV) (8.02% and 1.82%, respectively). Three kinds of cows, Luxi Yellow cows, Holstein heifers and Holstein cows with normal reproductive records were used as recipients. When the reconstructed embryos from GN were transferred, there was no difference in the pregnancy rate among three kinds of recipients, but the abortion rate of Luxi Yellow cows was significantly higher (85.71%) than in the other two groups (14.29% and 0%, respectively; P < 0.05). And the percentages of newborn calves in transferred embryos were significantly different between Luxi Yellow cows and Holstein breed (1.54%, 10.39% and 20.0%, respectively, P < 0.05). However, when reconstructed embryos from GLV were transferred, there was no difference among three kinds of recipients in the pregnancy rate, the abortion rate and the delivery rate.


bovine somatic cell nuclear transfer reconstructed embryo clone 


  1. 1.
    Gurdon, J. B., Laskey, R. A., Reeves, O. R., The developmental capacity of nuclei transplanted from keratinized skin cells of adult frogs, J. Embryol. Exp. Morph., 1975, 34: 93–112.PubMedGoogle Scholar
  2. 2.
    Campbell, K. H. S., McWhir, J., Ritchie, W. A. et al., Sheep cloned by nuclear transfer from a cultured cell line, Nature, 1996, 380: 64–66.PubMedCrossRefGoogle Scholar
  3. 3.
    Wilmut, I., Schnieke, A. E., McWhir, J. et al., Viable offspring derived from fetal and adult mammalian cells, Nature, 1997, 853: 810–813.CrossRefGoogle Scholar
  4. 4.
    Wakayama, T., Perry, A. C., Zuccotti, M. et al., Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei, Nature, 1998, 394: 369–374.PubMedCrossRefGoogle Scholar
  5. 5.
    Wang, Y. G., Zhou, X. G., Liu, J. et al., Cloned goats (Capra hircus) from foetal fibroblast cell lines, Chinese Science Bulletin, 1999, 45: 34–38.CrossRefGoogle Scholar
  6. 6.
    Cibelli, J. B., Stice, S. L., Golucke, P. J. et al., Cloned transgenic calves produced from nonquiescent fetal fibroblasts, Science, 1998, 280: 1256–1258.PubMedCrossRefGoogle Scholar
  7. 7.
    Polejaeva, I. A., Chen, S. H., Vaught, T. D. et al., Cloned pigs produced by nuclear transfer from adult somatic cells, Nature, 2000, 407: 505–509.CrossRefGoogle Scholar
  8. 8.
    Shin, T., Kraemer, D., Pryor, J. et al., A cat cloned by nuclear transplantation, Nature, 2002, 415: 859.PubMedCrossRefGoogle Scholar
  9. 9.
    Chesné, P., Adenot, P. G., Viglietta, C. et al., Cloned rabbits produced by nuclear transfer from adult somatic cells, Nat. Biotechnol., 2002, 20: 366–369.PubMedCrossRefGoogle Scholar
  10. 10.
    Frehney, R. I., Culture of animal cells, 3rd. New York: Wiley-Liss Inc, 1994, 127–147.Google Scholar
  11. 11.
    Chen, D. Y., Sun, Q. Y., Liu, J. L. et al., The giant panda (Ailuropoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg, Science in China, Series C, 1999, 42: 346–353.PubMedGoogle Scholar
  12. 12.
    Wells, D. N., Misica, P. M., Tervit, H. R. et al., Adult somatic cell nuclear transfer is used to preserve the last surviving cow of the Enderby Island cattle breed, Reprod. Fertil. Dev., 1998a, 10: 369–378.PubMedCrossRefGoogle Scholar
  13. 13.
    Kato, Y., Tani, T., Sotomaru, Y. et al., Eight calves cloned from somatic cells of a single adult, Science, 1998, 282: 2095–2098.PubMedCrossRefGoogle Scholar
  14. 14.
    Kubota, C., Yamakuchi, H., Todoroki, T. et al., Six cloned calves produced from adult fibroblast cells after long-term culture, Proc. Natl. Acad. Sci. USA, 2000, 97: 990–995.PubMedCrossRefGoogle Scholar
  15. 15.
    An Xiaorong, Gou Kemian, Zhu Shien et al. Cloned calves produced by nuclear transfer from cultured cumulus cells, Science in China, Series C, 2002, 45(2): 201–210.Google Scholar
  16. 16.
    Wells, D. N., Misica, P. M., McMillan, W. H. et al., Production of cloned bovine fetuses following nuclear transfer with cells from a fetal fibroblast cell line, Theriogenology, 1998b, 49: 330 (abs).CrossRefGoogle Scholar
  17. 17.
    Lanza, R. P., Cibelli, J. B., Blackwell, C. et al., Extension of cell life-span and telomere length in animals cloned from senescent somatic cells, Science, 2000a, 288: 665–669.PubMedCrossRefGoogle Scholar
  18. 18.
    Kato, Y., Tani, T., Tsunoda, Y., Cloning of calves from various somatic cell types of male and female adult, newborn and fetal cows, J. Reprod. Fertil., 2000, 120: 231–237.PubMedCrossRefGoogle Scholar
  19. 19.
    Hill, J. R., Roussel, A. J., Cibelli, J. B. et al., Clinical and pathologic features of cloned transgenic calves and fetuses (13 case studies), Theriogenolgoy, 1999, 51: 1451–1465.CrossRefGoogle Scholar
  20. 20.
    Hill, J. R., Burghard, R. C., Jones, K. et al., Evidence for placental abnormality as a major cause of mortality in first-trimester somatic cell cloned bovine fetuses, Biol. Reprod. 2000, 63: 1787–1794.PubMedCrossRefGoogle Scholar
  21. 21.
    Sousa, P. A. D., King, T., Harkness, L. et al., Evaluation of gestational deficiencies in cloned sheep fetuses and placentae, Biol. Reprod., 2001, 65: 23–30.PubMedCrossRefGoogle Scholar
  22. 22.
    Amano, T., Kato, Y., Tsunoda, Y., Full-term development of enucleated mouse oocytes fused with embryonic stem cells form different cell lines, Reproduction, 2001, 121: 729–733.PubMedCrossRefGoogle Scholar
  23. 23.
    Keefer, C. L., Baldassare, H., Keyston, R. et al., Generation of dwarf goat (Capra hircus) clones following nuclear transfer with transfected and nontransfected fetal fibroblasts and in vitro-matured oocytes. Biol. Reprod., 2001, 64: 849–856.PubMedCrossRefGoogle Scholar
  24. 24.
    Keefer, C. L., Keyston, R., Lazaris, A. et al., Production of cloned goats after nuclear transfer using adult somatic cells, Biol. Reprod., 2002, 66: 199–203.PubMedCrossRefGoogle Scholar
  25. 25.
    GUO Jitong, AN Zhixing, LI Yu et al. Cloned goats (Capra hircus) from adult ear cells, Science in China, Series C, 2002, 45(3): 260–267.Google Scholar
  26. 26.
    Betthauser, J., Forsberg, E., Augenstein, M. et al., Production of cloned pigs from in vitro systems, Nat. Biotechnol., 2000, 18: 1055–1059.PubMedCrossRefGoogle Scholar
  27. 27.
    Olsen, T. A. The genetic basis for piebald patterns in cattle, J. Hered., 1981, 72: 113–116.Google Scholar

Copyright information

© Science in China Press 2003

Authors and Affiliations

  • Dayuan Chen
    • 1
  • Jinsong Li
    • 1
    • 3
  • Zhiming Han
    • 1
  • Lei Lei
    • 1
  • Zhonghua Liu
    • 1
  • Zhaohui Kou
    • 1
  • Shiyuan Ma
    • 2
  • Qike Du
    • 2
  • Qingyuan Sun
    • 1
  1. 1.State Key Laboratory of Reproductive Biology, Institute of ZoologyChinese Academy of SciencesBeijingChina
  2. 2.The Center for Animal Embryo EngineeringZhongda GroupCaoxianChina
  3. 3.College of Animal Science and Veterinary MedicineYangzhou UniversityYangzhouChina

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