Science in China Series C: Life Sciences

, Volume 45, Issue 5, pp 546–552 | Cite as

Screening of BRD7 interacting proteins by yeast two-hybrid system

  • Ying Yu
  • Shiguo Zhu
  • Bicheng Zhang
  • Ming Zhou
  • Xiaoling Li
  • Guiyuan Li
Article
  • 34 Downloads

Abstract

BRD7 gene is low or undetectably expressed in nasopharyngeal carcinoma tissue (NPC) and can obviously suppress the growth of NPC cell line HNE1. In this study, the proteins that interacted with BRD7 coding protein were screened by yeast two-hybrid system. The complete reading frame of BRD7 gene was subcloned into pAS2 vector (BRD7-BD). Then the human embryo brain cDNA library was screened by BRD7-BD bait. Eleven positive clones were obtained from 4.8 × 106 transformed clones. By sequencing directly, 6 interacting proteins of BRD7 coding protein were isolated (Bromodomain-containing 3 protein (BRD3), Bromodomain-containing 2 protein (BRD2), I κB kinase-beta, KIAA1375 protein, interleukin 7 and adaptor-related protein complex 3 δ-1 subunit). These results suggest BRD7 protein might form heterogenous dimer or triplex polymer with BRD2 and/or BRD3 to participate in transcriptional regulation.

Keywords

nasopharyngeal carcinoma BRD7 two-hybrid system interaction 

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Copyright information

© Science in China Press 2002

Authors and Affiliations

  • Ying Yu
    • 1
  • Shiguo Zhu
    • 1
  • Bicheng Zhang
    • 1
  • Ming Zhou
    • 1
  • Xiaoling Li
    • 1
  • Guiyuan Li
    • 1
  1. 1.Cancer Research Institute, Xiangya School of MedicineCentral South UniversityChangshaChina

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