Chinese Science Bulletin

, 47:1277 | Cite as

Nuclear transplantation in different strains of zebrafish

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Abstract

Single later blastula nuclei from AB strain of zebrafish (Danio rerio) were transplanted into enucleated unfertilized eggs of Long fin strain. Of 1119 cloning embryos, 14 reconstructed embryos developed into fry. DNA fingerprinting systems of the cloned fish were similar to those of the nuclear donor fish, but were distinctly different from those of the nuclear recipient fish. It confirmed that the genetic material originated from nuclear donor cell other than from nuclear recipient egg. The research suggested that the basic technique for nuclear transplantation performed with different strains of zebrafish has made a breakthrough. It should be helpful for the study of some important developmental problems such as gene function, the regulation ogene expression during animal development, the developmental potential of a nucleus and the interactions between the donor nucleus and the recipient cytoplasm, etc.

Keywords

Zebrafish different strains nuclear transplantation DNA fingerprint 

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Copyright information

© Science in China Press 2002

Authors and Affiliations

  • Wei Hu
    • 1
  • Yaping Wang
    • 1
  • Shangping Chen
    • 1
  • Zuoyan Zhu
    • 1
  1. 1.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of HydrobiologyChinese Academy of SciencesWuhanChina

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