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Chinese Science Bulletin

, Volume 47, Issue 4, pp 307–309 | Cite as

Crystal structure of thermostable catechol 2,3-dioxygenase determined by multiwavelength anomalous dispersion method

  • Tao Jiang
  • Chaoneng Ji
  • Xiaoyu Sheng
  • Mingqin Chen
  • Yi Xie
  • Weimin Gong
  • Yumin Mao
Notes
  • 43 Downloads

Abstract

The selenomethionyl derivative of the thermostable catechol 2,3-dioxygenase (SeMet-TC23O) is expressed, purified and crystallized. By using multiwave length anomalous dispersion (MAD) phasing techniques, the crystal structure of TC23O at 0.3 nm resolutions is determined. TC23O is a homotetramer. Each monomer is composed of N-terminal and C-terminal domains (residues 1∼153 and 153∼319, respectively). The two domains are proximately symmetric by a non-crystallographic axis. Each domain contains two characteristic motifs which are found in almost all of extradial dioxygenases.

Keywords

multiwavelength anomalous dispersion (MAD) X-ray diffraction thermostable catechol 2,3-dioxygenase crystal structure synchrotron light source 

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Copyright information

© Science in China Press 2002

Authors and Affiliations

  1. 1.State Key Laboratory of Genetic Engineering, Institute of Genetics, School of life SciencesFudan UniversityShanghaiChina
  2. 2.Structural Biology Laboratory, School of life SciencesUniversity of Science and Technology of ChinaHefeiChina

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