Chinese Science Bulletin

, Volume 47, Issue 3, pp 199–201 | Cite as

Tobacco curly shoot virus isolated in Yunnan is a distinct species of Begomovirus

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Abstract

Virus isolate Y1 was obtained from tobacco showing curly shoot symptoms in Baoshan, Yunnan Province. Whitefly transmission test and virion morphology observation showed that it is a begomovirus. In reactions with 14 monoclonal antibodies raised against begomoviruses, Y1 was readily differentiated from begomoviruses reported in China, Pakistan and India. The complete nucleotide sequence of DNA-A was determined, it contains 2746 nucleotides, with two ORFs in virion-sense DNA and four ORFs in complementary-sense DNA. Comparisons with total DNA-A, intergenic region and deduced amino acid sequences of individual ORFs showed that Yl is a distinct Begomovirus species, for which the name Tobacco curly shoot virus (TCSV) is proposed. The total DNA-A of TCSV is most closely related to that of Tomato leaf curl virus from India (85% sequence identity). In contrast, the deduced coat protein of TCSV is most like that of Cotton leaf curl virus 72b isolate from Pakistan (98% amino acid sequence identity).

Keywords

Tobacco curly shoot virus Begomovirus distinct species recombination 

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Copyright information

© Science in China Press 2002

Authors and Affiliations

  • Yan Xie
    • 1
  • Xueping Zhou
    • 1
  • Zhongkai Zhang
    • 2
  • Yijun Qi
    • 1
  1. 1.Institute of BiotechnologyZhejiang UniversityHangzhouChina
  2. 2.Yunnan Biotechnology Research InstituteYunnan Academy of Agricultural SciencesKunmingChina

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