Tobacco curly shoot virus isolated in Yunnan is a distinct species of Begomovirus
- 57 Downloads
Virus isolate Y1 was obtained from tobacco showing curly shoot symptoms in Baoshan, Yunnan Province. Whitefly transmission test and virion morphology observation showed that it is a begomovirus. In reactions with 14 monoclonal antibodies raised against begomoviruses, Y1 was readily differentiated from begomoviruses reported in China, Pakistan and India. The complete nucleotide sequence of DNA-A was determined, it contains 2746 nucleotides, with two ORFs in virion-sense DNA and four ORFs in complementary-sense DNA. Comparisons with total DNA-A, intergenic region and deduced amino acid sequences of individual ORFs showed that Yl is a distinct Begomovirus species, for which the name Tobacco curly shoot virus (TCSV) is proposed. The total DNA-A of TCSV is most closely related to that of Tomato leaf curl virus from India (85% sequence identity). In contrast, the deduced coat protein of TCSV is most like that of Cotton leaf curl virus 72b isolate from Pakistan (98% amino acid sequence identity).
KeywordsTobacco curly shoot virus Begomovirus distinct species recombination
Unable to display preview. Download preview PDF.
- 2.Zhou, X. P., Liu, Y. L., Robinson, D. J. et al., Four DNA-A variants among Pakistani isolates of cotton leaf curl virus and their affinities to DNA-A of geminivirus isolates from okra, Journal of General Virology, 1998, 79: 915.Google Scholar
- 5.Zhou, X. P., Liu, Y. L., Calvert, L. et al., Evidence that DNA-A of a geminivirus associated with severe cassava mosaic disease in Uganda has arisen by interspecific recombination, Journal of General Virology, 1997, 78: 2101.Google Scholar
- 10.Hong, Y. G., Cai, J. H., Wang, X. F. et al., Chinese squash leaf curl virus: A new whitefly-transmitted geminivirus, Science in China, Ser. B, 1995, 38(2): 179.Google Scholar
- 11.Hong, Y. G., Cai, J. H., Wang, X. F. et al., Preliminary study on molecular evolution of tobacco leaf curl geminivirus, Chinese Science Bulletin, 1994, 39(6): 520.Google Scholar
- 14.van Regenmortel, M. H. V., Fauquet, C. M., Bishop, D. H. L. et al., Virus Taxonomy: Seventh Report of the International Committee on Taxonomy of Virus, London: Academic Press, 2000.Google Scholar