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Transcritption regulation of soybean ribulose-1,5-bisphosphate carboxylase small subunit gene by external factors

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Chinese Science Bulletin

Abstract

Ribulose-1,5-bisphosphate carboxylase small subunit gene (rbcS) is present with multi-gene family in plant genome. In Glycine max, the rbcS polypeptide (EC4.1.1.39) is encoded by a gene family containing 4–8 members. Three full-length rbcS cDNA clones were isolated and characterized from soybean seedlings, and both of their nucleotide and amino acid sequences showed high similarity. Differential accumulation of the rbcS mRNA was observed among roots, hypocotyls, cotyledons, epicotyls and leaves. The rbcS genes were up-regulated by various external factors such as salicylic acid (SA), salt stress and drought stress. The expression level of rbcS genes after being treated by 2.0 mmol/L SA and 0.4% NaCl, respectively, is 2.5–3.0-fold as high as that of control sample. Moreover, soybean rbcS mRNA was accumulated with diurnal variation but easily influenced by light and low temperature.

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciences, 100101, Beijing, China

    Chaoying He, Weiquan Wang, Yang Dongfang, Jinsong Zhang & Shouyi Chen

  2. National Center for Soybean Improvement, Soybean Research Institute, Nanjing Agricultural University, 210095, Nanjing, China

    Junyi Gai

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  1. Chaoying He
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  2. Weiquan Wang
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  3. Yang Dongfang
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  4. Jinsong Zhang
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  5. Junyi Gai
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Correspondence to Shouyi Chen.

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He, C., Wang, W., Dongfang, Y. et al. Transcritption regulation of soybean ribulose-1,5-bisphosphate carboxylase small subunit gene by external factors. Chin.Sci.Bull. 47, 38–43 (2002). https://doi.org/10.1360/02tb9007

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  • DOI: https://doi.org/10.1360/02tb9007

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