Application of inter-simple sequence repeats to insect cell lines: Identification at the clonal andtissue-specific level

Articles Cell and Tissue Models

Summary

Inter-simple sequence repeat (ISSR) primers designed to anneal to microsatellites were used to obtain deoxyribonucleic acid (DNA) fingerprint profiles to distinguish among 16 established insect cell lines derived from an assortment of lepidopteran, dipteran, and coleopteran species. Three different levels of cell line comparison were made: (1) between parents and their clones, (2) among cell lines derived from different tissues from the same species, and (3) among cell lines derived from different insect species. Of the 16 repeat oligonucleotide primers used in this study, nine primers generated several unique markers to distinguish between parental cell lines and their clones. Four of the 16 primers also generated DNA profiles with a number of unique bands, enabling the distinction among cell lines derived from specific tissues from the same species. In addition, ISSR-generated DNA profiles provided the greatest number of unique markers to distinguish easily among insect cell lines derived from different species.

Key words

inter-simple sequence repeats ISSR insect cell line DNA fingerprint 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abbot, P. Individual and population variation in invertebrates reyealed by inter-simple sequence repeats (ISSRs). J. Insect Sci. 1(8):58–60 www.insectscience.org/1.8; 2001.Google Scholar
  2. Hink, W. F. Established insect cell line from the cabbage looper, Trichoplusiani. Nature (Lond.) 226:466–467; 1970.CrossRefGoogle Scholar
  3. Jaccard, P. Nouvelles recheches sur la distribution florale. Bull. Soc. Vaudoise Sci. Nat. 44:223–270; 1908.Google Scholar
  4. Kariuki, C. W.; McIntosh, A. H.; Goodman, C. L. In vitro host range studies with a new baculovirus isolate from the diamondback moth Plutella xylostella (L.) (Plutellidae: Lepidoptera). In Vitro Cell. Dev. Biol. 36:271–276; 2000.CrossRefGoogle Scholar
  5. Kawai, Y.; Matsuhashi, J. An insect cell line discrimination method by RAPD-PCR. In Vitro Cell. Dev. Biol. 33:512–515; 1997.Google Scholar
  6. Kumar, L. S.; Sawant, A. S.; Gupta, V. S.; Ranjekar, P. K. Comparative analysis of genetic diversity among Indian populations of Scirpophaga incertulas by ISSR-PCR and RAPD-PCR. Biochem. Genet. 39:297–309; 2001.PubMedCrossRefGoogle Scholar
  7. Lenz, C. J.; McIntosh, A. H.; Mazzacano, C.; Munderloh, U. Replication of Heliothis zea nuclear polyhedrosis virus in cloned cell lines. J. Invertebr. Pathol. 57:227–233; 1991.PubMedCrossRefGoogle Scholar
  8. Long, S. L.; McIntosh, A. H.; Grasela, J. J.; Goodman, C. L. The establishment of a Colorado potato beetle (Coleoptera: Chrysomelidae) pupal cell line. Appl. Entomol. Zool. 37(3):447–450; 2002.CrossRefGoogle Scholar
  9. McIntosh, A. H.; Grasela, J. J.; Matteri, R. Identification of insect cell lines by DNA amplification fingerprinting (DAF). Insect Mol. Biol. 5:187–195; 1996.PubMedGoogle Scholar
  10. McIntosh, A. H.; Ignoffo, C. M. Characterization of five cell lines established from species of Heliothis. Appl. Entomol. Zool. 18:262–269; 1983.Google Scholar
  11. McIntosh, A. H.; Ignoffo, C. M.; Quhou, C.; Pappas, M. Establishment of a cell line from Heliothis armigera (Hbn) (Lepidopteran: Noctuidae). In Vitro 19:589–590; 1983.CrossRefGoogle Scholar
  12. Pringle, F. M.; Johnson, K. N.; Goodman, C. L.; McIntosh, A. H.; Bell, L. A. Providence virus: a new member of the Tetraveridae that infects cultured insect cells. Virology 306:359–370; 2003.PubMedCrossRefGoogle Scholar
  13. Reddy, K. D.; Nagarju, J.; Abraham, E. G. Genetic characterization of the silkworm Bombyx mori by simple sequence repeat (SSR)-anchored PCR. Heredity 83:681–687; 1999.PubMedCrossRefGoogle Scholar
  14. Schneider, I. Cell lines from late embryonic stages of Drosophila melanogaster. J. Embryol. Exp. Morphol. 27:353–365; 1972.PubMedGoogle Scholar
  15. Singh, K. R. P. Cell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.). Curr. Sci. 36:506–508; 1967.Google Scholar
  16. Vaughn, J. L.; Goodwin, R. G.; Tompkins, G. J.; McCawley, P. The establishment of two insect cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro 13:213–217; 1977.PubMedCrossRefGoogle Scholar
  17. Wolfe, A. D.; Liston, A. Contributions of PCR-based methods to plant systematics and evolutionary biology. In: Soltis, D. E.; Soltis, P. S.; Doyle, J. J., ed. Molecular systematics of plants II: DNA sequencing, New York: Kluwer, 1998:43–86.Google Scholar
  18. Zietkiewicz, E.; Fafalski, A.; Labuda, D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20:176–183; 1994.PubMedCrossRefGoogle Scholar

Copyright information

© Society for In Vitro Biology 2003

Authors and Affiliations

  1. 1.Biological Control of Insects Research Laboratory, Agricultural Research ServiceU.S. Department of AgricultureColumbia

Personalised recommendations