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Effects of rare earth compounds on growth and apoptosis of leukemic cell lines

  • Yucheng Dai
  • Jian Li
  • Jie Li
  • Li Yu
  • Ge Dai
  • Aiguo Hu
  • Liya Yuan
  • Zhu Wen
Reports

Summary

To explore a new agent for inhibiting leukemic cells, we investigated the effects of rare earth compounds (lanthanum chloride and cerium chloride) on the growth and apoptosis of HL-60 and NB4 cells. The growth of HL-60 and NB4 cells was tested by 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide (MTT) colorimetric assay. The apoptosis was measured by light microscopy, flow cytometry, and terminal deoxynucleotidyl transferase—mediated deoxyuridine triphosphate (dUTP) nick-end labeling (TUNEL) method. The effect of LaCl3 on normal bone marrow hematopoietic progenitor cells was evaluated by colony-forming unit-granulocyte-macrophage (CFU-GM) assay. Under our experimental conditions, MTT assay showed that 48-h treatment with 1, 2, and 3 mM LaCl3 or 48- and 72-h treatments with 1 mM LaCl3 could significantly inhibit the growth of HL-60 cells. Treatment with 2 and 4 mM CeCl3 for 72 h could significantly inhibit the growth of NB4 cells. Apoptosis could be detected on treatment with 2 mM LaCl3 for 24 h in HL-60 cells by light microscopic morphology examination, flow cytometric analysis, and TUNEL method. Apoptosis could be also detected on treatment with 2 mM CeCl3 for 72 h in NB4 cells. Treatment with 1 mM LaCl3 could arrest the transitions from GO/G1 to S phase. The granulocyte-macrophage colony formation of normal bone marrow cells was not significantly inhibited at lower concentrations of LaCl3 (0.5 to 2 mM). Our results indicate that at certain concentrations, the rare earth compounds may inhibit the growth of leukemic cells, induce them to apoptosis, and have no significant inhibitory effects on normal bone marrow hematopoietic progenitor cells (CFU-GM). The mechanism needs to be further investigated.

Key words

lanthanum chloride cerium chloride HL-60 cells NB4 cells apoptosis 

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References

  1. Anghileri, L. J. On the antitumor activity of gallium and lanthanides. Arzneimittelforschung 25:793–795; 1975.PubMedGoogle Scholar
  2. Hall, J.; Husken, D.; Haner, R. Towards artificial ribonucleases: the sequence-specific cleavage of RNA in a duplex. Nucleic Acids Res. 24:3522–3526; 1996.PubMedCrossRefGoogle Scholar
  3. Liu, L. S.; Wang, P.; Wan, S. Z.; Zheng, R. L.; Zhang, L. D.; Chen, S. Y.; Wang, C. H.; Hou, Z. J. Effects of complex of 3,6-di-(dimethylamino)-dibenzopyriodonium with praseodymium dicitrate on the syntheses of DNA, RNA, protein, nucleoprotein, and ATP of leukemia L 7712 cells in mice. Acta Pharmacol. Sinica 10:188–191; 1989.Google Scholar
  4. Sato, T.; Hashizume, M.; Hotta, Y.; Okahata, Y. Morphology and proliferation of melanoma cells in the presence of lanthanoid and Al3+ ions. Biometals 11:107–112; 1998.PubMedCrossRefGoogle Scholar
  5. Xiao, B.; Ji, Y.; Cui, M. Effects of lanthanum and cerium on malignant proliferation and expression of tumor-related gene. Chin. J. Prev. Med. 31;228–230; 1997.Google Scholar

Copyright information

© Society for In Vitro Biology 2002

Authors and Affiliations

  • Yucheng Dai
    • 1
    • 2
  • Jian Li
    • 1
    • 2
  • Jie Li
    • 1
    • 2
  • Li Yu
    • 1
    • 2
  • Ge Dai
    • 1
    • 2
  • Aiguo Hu
    • 1
    • 2
  • Liya Yuan
    • 1
    • 2
  • Zhu Wen
    • 1
    • 2
  1. 1.Department of Hematology Research LaboratorySecond Affiliated Hospital of Jiangxi Medical CollegeNanchangP.R. China
  2. 2.Jiangxi Institute of Medical SciencesNanchangP.R. China

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