Penaeid (Penaeus japonicus) lymphoid cells replicate by cell division in vitro

  • Gang-Hua Lang
  • Nakao Nomura
  • Bi-Zhao Wang
  • Masatoshi Matsumura


Penaeid cell culture has gained much attention as a potential model to facilitate researches on the characterization of the virus and to develop more sophisticated and improved diagnostic procedures for use in the aquaculture industry. However, to date, cell division processes of cultured penaeid cells have not been found, which is suggested as one of the reasons that block the establishment of the continuous penaeid cell lines. We reported here the cell division processes of cultured lymphoid cells of Penaeus japonicus. The culture medium used was based on M199 and was modified by supplementing saline components. Cultures were incubated at 25°C, and 5% CO2 was supplemented. In primary cultured lymphoid cells, dividing cells in different shapes were found. Cell division processes of 12 dividing lymphoid cells were tracked. After cell division, their daughter cells turned into fibroblast-like or epithelioid cells. These results proved that the culture conditions used were suitable for lymphoid cells of P. japonicus to proliferate in vitro and that cultured lymphoid cells still had the ability to carry out cell division. These findings would give light to the establishment of continuous penaeid cell lines and would also provide us with the knowledge of cell division processes of the penaeid.

Key words

penaeid cell culture fibroblast epithelioid mitosis cell cycle 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Braasch, D. A.; Ellender, R. D.; Middlebrooks B. L. Cell cycle components and their potential impact on the development of continuous in vitro penaeid cell replication. Meth. Cell. Sci. 21:255–261; 1999.CrossRefGoogle Scholar
  2. Chen, S. N.; Chi, S. C.; Kou, G. H.; Liao, I. C. Cell culture from tissues of grass prawn, Penaeus monodon. Fish Pathol. 21:161–166; 1986.Google Scholar
  3. Fraser, C. A.; Hall, M. R. Studies on primary cell cultures derived from ovarian tissue of Penaeus monodon. Methods Cell Sci. 21:213–218; 1999.PubMedCrossRefGoogle Scholar
  4. Hu, K. Studies on a cell culture from the hepatopancreas of the oriental shrimp, Penaeus shrimp, Penaeus orientalis Kishinouye. Asian Fish. Sci. 2:299–307; 1990.Google Scholar
  5. Itami, T.; Aoki, Y.; Hayashi, K.; Yu, Y.; Takahashoi, Y. In vitro maintenance of cells of lymphopoid organ in kuruma shrimp Penaeus japonicus. Nippon Suisan Gakkaishi 55:2205; 1989.Google Scholar
  6. Lang, G.-H.; Wang, Y.; Wang, M.; Chen, X.-G. The long-term primary culture of Penaeus chinesis lymphoid tissue and a primary study of its chemical transformation [in Chinese, with English abstract]. J. Ocean Univ. Qingdao 31(3):411–414; 2001.Google Scholar
  7. Luedeman, R. A.; Lightner, D. V. Development of an in vitro primary cell culture system from the penaeid shrimp, Penaeus stylirostris and P. vannamei. Aquaculture 101:205–211; 1992.CrossRefGoogle Scholar
  8. Mark, S. T.; Crane, J. Mutagenesis and cell transformation in cell culture. Methods Cell Sci. 21:243–253; 1999.Google Scholar
  9. Nadala, E. C. B.; Lu, Y.; Loh, P. C. Primary culture of lymphoid, nerve and ovary cells from Penaeus stylirostris and Penaeus vannamei. In Vitro Cell Dev. Biol. 29A:620–622; 1993.Google Scholar
  10. Najafabadi, A. K.; Ellender, R. D.; Middlebrooks, B. L. Analysis of shrimp hemolymph and ionic modification of a penaeus cell culture formulation. J. Aquat. Anim. Health 4:143–148; 1992.CrossRefGoogle Scholar
  11. Rosenthal, J.; Diamant, A.: In vitro primary cell cultures from Penaeus semisulcatus. In: Perkins, F. O.; Cheng, T. C., ed. Pathology in marine science, New York: Academic Press; 1990:7–13.Google Scholar
  12. Shimizu, C.; Shike, H.; Klempel, K. R.; Burns, J. K. Hemolymph analysis and evaluation of newly formulated medium for culture of shrimp cells (Penaeus stylirostris) In Vitro Cell Dev. Biol. 37A (6):322–329; 2001.Google Scholar
  13. Tapay, L. M.; Lu, Y.; Brock, J. A.; Nadala, E. C. B.; Loh, P. C. Transformation of primary cultures of shrimp (Penaeus stylirostris) lymphoid (oka) organ with simian virus-40 (T) antigen. Soc. Exp. Bio. Med. 209:73–78; 1995.Google Scholar
  14. Tong, S. L.; Miao, H. Z. Attempt to initiate cell cultures from Penaeus chinensis tissues. Aquaculture 147:151–157; 1996.CrossRefGoogle Scholar
  15. Toullec, J. Y. Crustacean primary cell culture: a technical approach. Methods Cell Sci. 21:193–198 1999.PubMedCrossRefGoogle Scholar
  16. Whiteley, J. M.; Taylor, E. W. The effects of seasonal variations in temperature of extracellular acid-base status in a wild population of the crayfish Austrapotamobius pallipes. J. Exp. Biol. 181:295–311; 1993.Google Scholar

Copyright information

© Society for In Vitro Biology 2002

Authors and Affiliations

  • Gang-Hua Lang
    • 1
    • 3
  • Nakao Nomura
    • 2
  • Bi-Zhao Wang
    • 1
  • Masatoshi Matsumura
    • 1
  1. 1.Institute of Applied BiochemistryUniversity of TsukubaTsukuba, IbarakiJapan
  2. 2.The Science and Technology Promotion Foundation of IbarakiTsukuba, IbarakiJapan
  3. 3.College of Marine Life SciencesOcean University of QingdaoQingdaoPeople’s Republic of China

Personalised recommendations