Penaeid (Penaeus japonicus) lymphoid cells replicate by cell division in vitro

  • Gang-Hua Lang
  • Nakao Nomura
  • Bi-Zhao Wang
  • Masatoshi Matsumura
Reports

Summary

Penaeid cell culture has gained much attention as a potential model to facilitate researches on the characterization of the virus and to develop more sophisticated and improved diagnostic procedures for use in the aquaculture industry. However, to date, cell division processes of cultured penaeid cells have not been found, which is suggested as one of the reasons that block the establishment of the continuous penaeid cell lines. We reported here the cell division processes of cultured lymphoid cells of Penaeus japonicus. The culture medium used was based on M199 and was modified by supplementing saline components. Cultures were incubated at 25°C, and 5% CO2 was supplemented. In primary cultured lymphoid cells, dividing cells in different shapes were found. Cell division processes of 12 dividing lymphoid cells were tracked. After cell division, their daughter cells turned into fibroblast-like or epithelioid cells. These results proved that the culture conditions used were suitable for lymphoid cells of P. japonicus to proliferate in vitro and that cultured lymphoid cells still had the ability to carry out cell division. These findings would give light to the establishment of continuous penaeid cell lines and would also provide us with the knowledge of cell division processes of the penaeid.

Key words

penaeid cell culture fibroblast epithelioid mitosis cell cycle 

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Copyright information

© Society for In Vitro Biology 2002

Authors and Affiliations

  • Gang-Hua Lang
    • 1
    • 3
  • Nakao Nomura
    • 2
  • Bi-Zhao Wang
    • 1
  • Masatoshi Matsumura
    • 1
  1. 1.Institute of Applied BiochemistryUniversity of TsukubaTsukuba, IbarakiJapan
  2. 2.The Science and Technology Promotion Foundation of IbarakiTsukuba, IbarakiJapan
  3. 3.College of Marine Life SciencesOcean University of QingdaoQingdaoPeople’s Republic of China

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