In vitro host range studies with a new baculovirus isolate from the diamondback moth Plutella xylostella (L.) (Plutellidae: Lepidoptera)
The in vitro host range of a newly isolated baculovirus from the diamondback moth Plutella xylostella was tested against six lepidopteran cell lines. Two baculoviruses with host ranges from the alfalfa looper Autographa californica (A. californica multiple nucleopolyhedrovirus, AcMNPV) and the celery looper Anagrapha falcifera (AfMNPV) were also included in this study for comparative purposes. PxMNPV replicated in all six cell lines and produced occlusion bodies, with HV-AMI and TN-CLI cells producing the highest viral titers and greatest number of occlusion bodies. There was no significant replication of AcMNPV and AfMNPV in the HZ-FB33 cell line and thus no production of occlusion bodies. The restriction endonuclease profiles of the three baculoviruses showed similarities but could be readily distinguished from each other. Either HV-AM1 or TN-CL1 would be suitable cell lines for the in vitro production of PxMNPV.
Key wordslepidopteran cell lines PxMNPV
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- Agathos, S.N. Large scale insect cell production. In: Maramorosch, K.; McIntosh, A., ed. Insect cell biotechnology, Boca Raton, FL. CRC Press; 1994.Google Scholar
- Belisle, B. W.; Walls, E. L.; Celeri, C. Knoch, C.; Singer, V.; Tang, K. From shake flask to industrial fermentor: challenges of large scale culture. In Vitro Cell. Dev. Biol. 28A (48A); 1992.Google Scholar
- Hostetter, D. L.; Puttler, B. A new broad host spectrum nuclear polyhedrosis virus isolated from a celery looper, Anagrapha falcifera (Kirby) (Lepidoptera Noctuidae). Environ. Entomol. 20:1480–1488; 1991.Google Scholar
- McIntosh, A. H.; Andrews, P. A.; Ignoffo, C. M. Establishment of two continuous cell lines of Heliothis virescens (F.) (Lepidoptera: Noctuidae). In Vitro 17(18):649–650; 1981.Google Scholar
- Weiss, S. A.; Godwin, G. P.; Whitford, W. G.; Dougherty, E. M.; Rohrman, G. F. Viral pesticides: production in serum-free insect cell culture. In Vitro Cell. Dev. Biol. 28A(87A); 1992.Google Scholar
- Weiss, S. A.; Vaughn, J. L. Cell culture methods for large-scale propagation of baculoviruses. In: Granados, R. R.; Federici, B. A., ed. The biology of baculoviruses. Vol. II. Boca Raton, FL: CRC Press; 1986;63.Google Scholar