Effect of dexamethasone on binding activity of transcription factors nuclear factor-κB and activator protein-1 in SW982 human synovial sarcoma cells
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Previously, we showed that dexamethasone (DEX) inhibited the expression of inflammatory cytokines, matrix metalloproteinase-1, and cyclooxygenase-2 messenger ribonucleic acid in SW982 cells. In this study, the effect of DEX on the transcription factors nuclear factor-κB (NF-κB) and activator protein-1 (AP-1) was examined in SW982 cells by electrophoretic mobility shift assay (EMSA). Both NF-κB and AP-1 deoxyribonucleic acid binding activities were detectable in SW982 cells by EMSA, and they were induced by interleukin-1β treatment. DEX inhibited NF-κB binding activity at 10 μM as well as at 100 μM, although the inhibition was only partial. However, DEX had little effect on AP-1 activity. These results suggest that DEX reduces the expression of inflammatory cytokines and other proteins in SW982 cells by inhibiting NF-κB.
Key wordscell line anti-inflammatory agent transcription DNA binding assay
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