Abstract
Background
A recent study reported that long non-coding RNA activated by TGF-β (lncRNA-ATB) induced epithelial–mesenchymal transition (EMT) through the transforming growth factor-β (TGF-β)/miR-200s/ZEB axis in hepatocellular carcinoma. Herein, we focused on the clinical significance of lncRNA-ATB in gastric cancer (GC) patients.
Materials and Methods
Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to examine expression of lncRNA-ATB, miR-200b, and miR-200c in GC tissues (n = 183). Patients were divided into high and low lncRNA-ATB expression groups using a cutoff of lncRNA-ATB/GAPDH ≥0.60 or <0.60 to determine the clinicopathological significance of lncRNA-ATB in GC. Moreover, we evaluated the expression of TGF-β, lncRNA-ATB, miR-200s, and ZEB1 in GC cell lines by qRT-PCR. GC cell lines were treated by recombinant TGF-β1 or TGF-β receptor inhibitor to examine morphologic changes and genetic alterations, such as lncRNA-ATB, miR-200s, and ZEB1 levels, with respect to the EMT phenotype.
Results
The high lncRNA-ATB group experienced a lower overall survival rate compared with the low lncRNA-ATB group, and multivariate analysis indicated that lncRNA-ATB was an independent prognostic factor (hazard ratio 3.50; 95 % CI 1.73–7.44; p = 0.0004). miR-200c levels were lower and ZEB1 levels were higher in the high lncRNA-ATB group than in the low lncRNA-ATB group. Treatment with TGF-β in GC cell lines resulted in morphological EMT changes, upregulation of lncRNA-ATB and ZEB1, and downregulation of miR-200c and CDH1. SB431542 reduced lncRNA-ATB expression.
Conclusion
LncRNA-ATB plays an important role in EMT to promote invasion and metastasis through the TGF-β/miR-200s/ZEB axis, resulting in a poor prognosis in GC. LncRNA-ATB is a novel biomarker of lncRNA, indicative of a poor prognosis in GC patients.
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References
Nagini S. Carcinoma of the stomach: a review of epidemiology, pathogenesis, molecular genetics and chemoprevention. World J Gastrointest Oncol. 2012;4:156–69.
Thiery JP. Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer. 2002;2:442–54.
Thiery JP. Epithelial-mesenchymal transitions in development and pathologies. Curr Opin Cell Biol. 2003;15:740–6.
Gregory PA, Bert AG, Paterson EL, et al. The MiR-200 family and MiR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1. Nat Cell Biol. 2008;10:593–601.
Wellner U, Schubert J, Burk UC, et al. The EMT-activator ZEB1 promotes tumorigenicity by repressing stemness-inhibiting microRNAs. Nat Cell Biol. 2009;11:1487–95.
Yu J, Ohuchida K, Mizumoto K, et al. MicroRNA, Hsa-miR-200c, is an independent prognostic factor in pancreatic cancer and its upregulation inhibits pancreatic cancer invasion but increases cell proliferation. Mol Cancer. 2010;9:169.
Hur K, Toiyama Y, Takahashi M, et al. MicroRNA-200c modulates epithelial-to-mesenchymal transition (EMT) in human colorectal cancer metastasis. Gut. 2013;62:1315–26.
Liu XG, Zhu WY, Huang YY, et al. High expression of serum MiR-21 and tumor MiR-200c associated with poor prognosis in patients with lung cancer. Med Oncol. 2012;29:618–26.
Cheng H, Zhang L, Cogdell DE, et al. Circulating plasma MiR-141 is a novel biomarker for metastatic colon cancer and predicts poor prognosis. PLoS One. 2011;6:e17745.
Kurashige J, Kamohara H, Watanabe M, et al. MicroRNA-200b regulates cell proliferation, invasion, and migration by directly targeting ZEB2 in gastric carcinoma. Ann Surg Oncol. 2012;19 Suppl 3:S656–64.
Burk U, Schubert J, Wellner U, et al. A reciprocal repression between ZEB1 and members of the MiR-200 family promotes EMT and invasion in cancer cells. EMBO Rep. 2008;9:582–9.
Prensner JR, Chinnaiyan AM. The emergence of LncRNAs in cancer biology. Cancer Discov. 2011;1:391–407.
Guttman M, Rinn JL. Modular regulatory principles of large non-coding RNAs. Nature. 2012;482:339–46.
Tsai MC, Manor O, Wan Y, et al. Long noncoding RNA as modular scaffold of histone modification complexes. Science. 2010;329:689–93.
Gupta RA, Shah N, Wang KC, et al. Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature. 2010;464:1071–6.
Matouk IJ, DeGroot N, Mezan S, et al. The H19 non-coding rna is essential for human tumor growth. PLoS One. 2007;2:e845.
Liu XH, Sun M, Nie FQ, et al. Lnc RNA HOTAIR functions as a competing endogenous RNA to regulate HER2 expression by sponging MiR-331-3p in gastric cancer. Mol Cancer. 2014;13:92.
Shao Y, Chen H, Jiang X, et al. Low expression of LncRNA-HmlincRNA717 in human gastric cancer and its clinical significances. Tumour Biol. 2014;35(10):9591–5.
Wang Y, Zhang D, Wu K, Zhao Q, Nie Y, Fan D. Long non-coding RNA MRUL promotes ABCB1 expression in multidrug-resistant gastric cancer cell sublines. Mol Cell Biol. 2014;34:3182–93.
Yuan JH, Yang F, Wang F, et al. A long noncoding RNA activated by TGF-beta promotes the invasion-metastasis cascade in hepatocellular carcinoma. Cancer Cell. 2014;25:666–81.
Kurokawa Y, Matsuura N, Kawabata R, et al. Prognostic impact of major receptor tyrosine kinase expression in gastric cancer. Ann Surg Oncol. 2014;21 Suppl 4:S584–90.
Hashiguchi Y, Nishida N, Mimori K, et al. Down-regulation of MiR-125a-3p in human gastric cancer and its clinicopathological significance. Int J Oncol. 2012;40:1477–82.
Tan Z, Jiang H, Wu Y, et al. MiR-185 is an independent prognosis factor and suppresses tumor metastasis in gastric cancer. Mol Cell Biochem. 2014;386:223–31.
Gregory PA, Bracken CP, Smith E, et al. An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition. Mol Biol Cell. 2011;22:1686–98.
Truong HH, Xiong J, Ghotra VP, et al. Beta1 integrin inhibition elicits a prometastatic switch through the TGFbeta-miR-200-ZEB network in E-cadherin-positive triple-negative breast cancer. Sci Signal. 2014;7:ra15.
Lamouille S, Xu J, Derynck R. Molecular mechanisms of epithelial-mesenchymal transition. Nat Rev Mol Cell Biol. 2014;15:178–96.
Acknowledgment
The authors would like to thank Dr. Kohei Miyazono, Department of Molecular Pathology, Graduate School of Medicine, the University of Tokyo, for his technical advice; and Ms. Kazumi Oda, Michiko Kasagi, and Satoko Kono, Kyshu University Beppu Hospital, for their technical support.
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10434_2015_4554_MOESM1_ESM.tif
Supplementary material 1 The subgroup analysis of the prognostic significance of the lncRNA-ATB among clinical stages. 22 cases with higher expression of lncRNA-ATB was significantly worse prognosis than 18 cases of lower expression of lncRNA-ATB (p=0.018).(TIFF 80 kb)
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Saito, T., Kurashige, J., Nambara, S. et al. A Long Non-coding RNA Activated by Transforming Growth Factor-β is an Independent Prognostic Marker of Gastric Cancer. Ann Surg Oncol 22 (Suppl 3), 915–922 (2015). https://doi.org/10.1245/s10434-015-4554-8
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DOI: https://doi.org/10.1245/s10434-015-4554-8