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Annals of Surgical Oncology

, Volume 21, Issue 7, pp 2361–2368 | Cite as

High Serum Levels of Vascular Endothelial Growth Factor-A and Transforming Growth Factor-β1 Before Neoadjuvant Chemoradiotherapy Predict Poor Outcomes in Patients with Esophageal Squamous Cell Carcinoma Receiving Combined Modality Therapy

  • Jason Chia-Hsien Cheng
  • Madeline S. Graber
  • Feng-Ming Hsu
  • Chiao-Ling Tsai
  • Leon Castaneda
  • Jang-Ming Lee
  • Daniel T. Chang
  • Albert C. Koong
Thoracic Oncology

Abstract

Background and Purpose

This study was aimed at using proximity ligation assay (PLA) followed by enzyme-linked immunosorbent assay (ELISA) to identify serum biomarkers that predict treatment response and survival for patients with esophageal squamous cell carcinoma (ESCC) undergoing neoadjuvant concurrent chemoradiotherapy (CCRT) followed by esophagectomy.

Methods

Seventy-nine patients with ESCC receiving CCRT of taxane-based/5-fluorouracil-based chemotherapy and 40 Gy followed by surgery were enrolled. Serum samples were collected before and <1 month after CCRT. Fifteen biomarkers were analyzed using PLA. Biomarkers significantly correlating with pathological response/survival were verified by ELISA. Associations of the serum level of biomarkers and clinical factors with pathological response, disease-free survival (DFS), and overall survival (OS) were evaluated by analysis of variance and log-rank tests.

Results

Thirty patients had complete response (38 %), 37 had microscopic residual disease (47 %), and 12 had macroscopic residual disease (15 %). With a median follow-up of 52.8 months, the median DFS was 43 months. Among the 15 biomarkers screened by PLA, vascular endothelial growth factor (VEGF)-A and transforming growth factor (TGF)-β1 were significantly associated with pathological response and/or DFS. These biomarkers were further analyzed by ELISA to confirm initial biomarker findings by PLA. After ELISA of these two markers, only VEGF-A levels were significantly correlated with pathological response. On multivariate analysis, patients with combined high pre-CCRT VEGF-A and TGF-β1 levels (greater than or equal to the median), independent of pathological response, had significantly worse DFS (11 months vs. median not reached; p = 0.007) and OS (16 vs. 46 months; p = 0.07).

Conclusions

Pre-CCRT serum VEGF-A and TGF-β1 levels may be used to predict pathological response and survivals for ESCC patients receiving combined-modality therapy.

Keywords

Vascular Endothelial Growth Factor Overall Survival Esophageal Squamous Cell Carcinoma Pathological Response Esophageal Squamous Cell Carcinoma Patient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Disclosures

Jason Chia-Hsien Cheng, Madeline S. Graber, Feng-Ming Hsu, Chiao-Ling Tsai, Leon Castaneda, Jang-Ming Lee, Daniel T. Chang, and Albert C. Koong declare no conflicts of interest or financial disclosures. This work was supported by National Science Council, Taiwan, ROC.

Supplementary material

10434_2014_3611_MOESM1_ESM.doc (50 kb)
Supplementary material 1 (DOC 50 kb)

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Copyright information

© Society of Surgical Oncology 2014

Authors and Affiliations

  • Jason Chia-Hsien Cheng
    • 1
    • 2
    • 4
  • Madeline S. Graber
    • 1
  • Feng-Ming Hsu
    • 2
  • Chiao-Ling Tsai
    • 2
  • Leon Castaneda
    • 1
  • Jang-Ming Lee
    • 3
  • Daniel T. Chang
    • 1
  • Albert C. Koong
    • 1
  1. 1.Department of Radiation OncologyStanford UniversityStanfordUSA
  2. 2.Division of Radiation Oncology, Department of OncologyNational Taiwan University HospitalTaipeiTaiwan
  3. 3.Department of SurgeryNational Taiwan University HospitalTaipeiTaiwan
  4. 4.Graduate Institute of OncologyNational Taiwan University College of MedicineTaipeiTaiwan

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