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Annals of Surgical Oncology

, Volume 20, Supplement 3, pp 406–414 | Cite as

miR-196a Overexpression and miR-196a2 Gene Polymorphism Are Prognostic Predictors of Oral Carcinomas

  • Chung-Ji Liu
  • Meng-Miao Tsai
  • Hsi-Feng Tu
  • Man-Tin Lui
  • Hui-Wen Cheng
  • Shu-Chun Lin
Translational Research and Biomarkers

Abstract

Background

Oral squamous cell carcinoma (OSCC) is prevalent worldwide, and survival in OSCC has not improved significantly in the last few decades. MicroRNAs have an important regulatory role in oral carcinogenesis. This study investigated the prognostic implications of miR-196 expression and the rs11614913 genotype of the miR-196a2 gene in OSCC.

Methods

The clinicopathologic implications of miR-196 in OSCC were investigated using expression assays and genotyping, and the functional role of miR-196 in OSCC pathogenesis was investigated using exogenous expression and knockdown.

Results

miR-196 was up-regulated in OSCC tissue relative to control mucosa. High expression of miR-196a, but not miR-196b, was associated with tumor recurrence, nodal metastasis, and mortality. Plasma miR-196a levels could be used to distinguish patients from controls with a separating power of 0.75. Multivariate analysis showed that both high miR-196a expression and TT genotype were independent predictors for poor survival in OSCC. The risk of mortality was greatest for patients with high miR-196a level and positive node status. Expression of miR-196 enhanced oncogenesis of OSCC cells, while inhibition of miR-196 expression attenuated such effects.

Conclusions

High miR-196a expression in tumor tissue and the presence of the TT variant of miR-196a2 gene indicate worse survival in OSCC.

Keywords

Oral Squamous Cell Carcinoma Oral Squamous Cell Carcinoma Oral Squamous Cell Carcinoma Cell Oral Squamous Cell Carcinoma Patient Fadu Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

Supported in part by grants NSC-99-2628-B-195-002-MY3, NSC-99-2628-B-010-013-MY3 and NSC100-2811-B-010-050 (Postdoc Training Grant for M.M.T.) from the National Science Council, Taiwan.

Supplementary material

10434_2012_2618_MOESM1_ESM.doc (1.3 mb)
Supplementary material 1 (DOC 1307 kb)

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Copyright information

© Society of Surgical Oncology 2012

Authors and Affiliations

  • Chung-Ji Liu
    • 1
    • 2
    • 3
    • 5
  • Meng-Miao Tsai
    • 5
  • Hsi-Feng Tu
    • 5
  • Man-Tin Lui
    • 4
    • 5
  • Hui-Wen Cheng
    • 3
  • Shu-Chun Lin
    • 4
    • 5
  1. 1.Department of Oral and Maxillofacial SurgeryMacKay Memorial HospitalTaipeiTaiwan, ROC
  2. 2.MacKay Medical CollegeTaipeiTaiwan, ROC
  3. 3.Department of Medical ResearchMacKay Memorial HospitalTaipeiTaiwan, ROC
  4. 4.Department of StomatologyTaipei Veterans General HospitalTaipeiTaiwan, ROC
  5. 5.Institute of Oral Biology, School of DentistryNational Yang-Ming UniversityTaipeiTaiwan, ROC

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