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Annals of Surgical Oncology

, Volume 19, Issue 5, pp 1595–1601 | Cite as

Lymphatic Vessels and High Endothelial Venules are Increased in the Sentinel Lymph Nodes of Patients with Oral Squamous Cell Carcinoma Before the Arrival of Tumor Cells

  • Man Ki Chung
  • In-Gu Do
  • Eunwook Jung
  • Young-Ik Son
  • Han-Sin Jeong
  • Chung-Hwan Baek
Head and Neck Oncology

Abstract

Purpose

To investigate the change of vasculature in the sentinel lymph node (SLN) of patients with oral squamous cell carcinoma.

Methods

Immunohistochemical staining of SLNs in 58 patients was performed with two monoclonal antibodies (MAb): anti-D2-40 MAb for lymphatic endothelial cells, and anti-MECA-79 MAb for high endothelial venules (HEV). Twelve metastatically involved (m+) SLNs, 120 uninvolved (m) SLNs, and 35 non-SLNs (control) were available for analyses. Vessel densities were measured by computer-assisted analyses in the entire region of SLN. Correlations were assessed between vessel density and clinicopathologic variables, including vascular endothelial growth factor C of primary tumor.

Results

Lymphatic vessel density (LVD) in SLNs was higher than that in control LNs [2361.8 μm2/high-power field (HPF) (624.3–4758.5) vs. 1621.9 μm2/HPF (465.3–3453.5), P = 0.005]. LVD of m SLNs [2662.4 μm2/HPF (624.3–4758.5)] and m± SLNs [4946.6 μm2/HPF (2009.3–8698.8)] were both statistically significantly higher compared to control. HEV densities in m SLNs [14029.7 μm2/HPF (10465.7–17927.1)] as well as m± SLNs [18258.5 μm2/HPF (8408.9–27706.0)] were also significantly higher than those in control [10350.5 μm2/HPF (7807.8–12541.1)]. By multivariate analysis, the degree of vascular endothelial growth factor C expression of primary tumor showed significant correlation with LVD of SLNs (odds ratio 9.46, 95% confidence interval 1.73–51.5, P = 0.009), which was not the case in HEV.

Conclusions

Lymphatic vessels and HEVs were increased in SLNs, regardless of metastatic status of SLNs. Vascular endothelial growth factor C expression of primary tumor may contribute to the premetastatic change within SLNs of oral squamous cell carcinoma.

Keywords

Vascular Endothelial Growth Factor Sentinel Lymph Node Sentinel Lymph Node Biopsy Oral Squamous Cell Carcinoma Lymphatic Vessel 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

This study was supported by Samsung Medical Center Clinical Research Development Program grant, #CRS 108-05-2.

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Copyright information

© Society of Surgical Oncology 2011

Authors and Affiliations

  • Man Ki Chung
    • 1
  • In-Gu Do
    • 2
  • Eunwook Jung
    • 1
  • Young-Ik Son
    • 1
  • Han-Sin Jeong
    • 1
  • Chung-Hwan Baek
    • 1
  1. 1.Department of Otorhinolaryngology-Head and Neck SurgerySungkyunkwan University School of Medicine, Samsung Medical CenterSeoulRepublic of Korea
  2. 2.Department of PathologySungkyunkwan University School of Medicine, Samsung Medical CenterSeoulRepublic of Korea

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