Clinical Significance of Human Kallikrein7 Gene Expression in Colorectal Cancer
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The human kallikrein-related peptidases (KLK) are considered important prognostic biomarkers in cancer. The aim of the current study is to demonstrate gene expression of KLK7 in colorectal cancer (CRC) and to correlate the relative KLK7 expression level with clinicopathological factors of CRC.
KLK7 messenger RNA (mRNA) expression was examined in nine CRC cancer cell lines by real-time polymerase chain reaction. The expression levels of KLK7 mRNA in cancerous tissues (n = 136) and paired normal tissues (n = 136) of CRC patients were also examined.
Six of the nine cell lines expressed the KLK7 gene. KLK7 mRNA expression levels in cancer tissues were significantly higher than those in normal tissues. Multivariate analysis revealed that the KLK7 mRNA expression level in cancer was an independent prognostic factor, especially in liver metastasis.
We provide evidence suggesting that KLK7 mRNA expression is correlated with prognosis in CRC patients, especially in liver metastasis.
KeywordsNoncancerous Tissue Paired Normal Tissue KLK7 Expression Cancer Genome Anatomy Project KLK7 mRNA
The funding sources supporting our work are as follows: Grant sponsor: Japan Society for the Promotion of Science (JSPS). Grant-in-Aid for Scientific Research; Grant numbers: 17109013,18659384, 18390367, 18590333, 19591509, 19390336, 20390360, 20591547, 20790961, 20659209, 20790960; Grant sponsor: The Ministry of Education, Culture, Sports, Science, and Technology (MEXT) Grant-in-Aid for Scientific Research on Priority Areas; Grant number: 18015039; Grant sponsor: Third-Term Comprehensive 10-year Strategy for Cancer Control; Grant number: 16271201; Grant sponsors: CREST, Japan Science and Technology Agency (JST); NEDO (New Energy and Industrial Technology Development Organization) Technological Development for Chromosome Analysis; The Ministry of Education, Culture, Sports, Science, and Technology of Japan for Scientific Research on Priority Areas, Cancer Translational Research Project, Japan; Grant of Clinical Research Foundation (2008–2010).
- 2.Japan treatment guidelines for colorectal cancer. Japanese Society for Cancer of the Colon and Rectum, editor, Kanehara Co., Tokyo; 2009.Google Scholar
- 3.Miki C, Inoue Y, Hiro J, Ojima E, Araki T, Uchida K, Kusunoki M. Combined measurement of hepatocyte growth factor and carcinoembryonic antigen as a prognostic marker for patients with dukes a and B colorectal cancer: results of a five-year study. Dis Colon Rectum. 2006;49(11):1710–8.CrossRefPubMedGoogle Scholar
- 31.Yousef GM, Scorilas A, Magklara A, Soosaipillai A, Diamandis EP. The KLK7 (PRSS6) gene, encoding for the stratum corneum chymotryptic enzyme is a new member of the human kallikrein gene family - genomic characterization, mapping, tissue expression and hormonal regulation. Gene. 2000;254(1–2):119–28.CrossRefPubMedGoogle Scholar