Annals of Surgical Oncology

, Volume 16, Issue 6, pp 1578–1586 | Cite as

Expression of P-aPKC-ι, E-Cadherin, and β-Catenin Related to Invasion and Metastasis in Hepatocellular Carcinoma

  • Guang-Sheng Du
  • Jian-Ming Wang
  • Jin-Xi Lu
  • Qiang Li
  • Chao-Qun Ma
  • Ji-Tao Du
  • Sheng-Quan Zou
Hepatobiliary and Pancreatic Tumors



Atypical protein kinase C iota (aPKC-ι) and its associated intracellular molecules, E-cadherin and β-catenin, are important for cell polarization in tumorigenesis and progression. Expression of aPKC-ι, P-aPKC-ι (activated aPKC-ι), E-cadherin, and β-catenin in hepatocellular carcinoma (HCC) was measured, and correlation with clinicopathological characteristics of HCC was analyzed.


Paraffin-embedded tumor tissue was obtained from patients with HCC after resection without preoperative radiotherapy or chemotherapy. Gene expression was detected by polymerase chain reaction (PCR), and protein expression was detected by immunohistochemistry and Western blot analysis. Expressions of aPKC-ι, P-aPKC-ι, E-cadherin, and β-catenin were analyzed with relation to the clinicopathological data.


The gene and protein expression of aPKC-ι are obviously higher in HCC tissues than that in peritumoral tissues and normal tissues by semiquantitative PCR and immunohistochemistry methods. Accumulation of aPKC-ι in HCC cytoplasm and nucleolus inhibited the later formation of belt-like adherens junctions (AJs) and/or tight junctions (TJs) in cell–cell contact. E-cadherin was reduced and accumulation of cytoplasm β-catenin was increased in HCC. The expression of aPKC-ι was closely related to pathological differentiation, tumor size, invasion, and metastasis of HCC.


Accumulation of cytoplasm aPKC-ι may reflect pathological differentiation, invasion, and metastasis potential of HCC. In this regard, our study on HCC revealed the potential usefulness of aPKC-ι, E-cadherin, and β-catenin as a prognostic marker, closely related to pathological differentiation, invasion, metastasis, and prognosis of HCC.


Hepatocellular Carcinoma Ethylene Diamine Tetraacetic Acid Ethylene Diamine Tetraacetic Acid Pathological Differentiation Peritumoral Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by grant no. 30672040 to Professor Jianming Wang, M.D. from the National Natural Science Foundation of China.


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Copyright information

© Society of Surgical Oncology 2009

Authors and Affiliations

  • Guang-Sheng Du
    • 1
  • Jian-Ming Wang
    • 1
  • Jin-Xi Lu
    • 1
  • Qiang Li
    • 1
  • Chao-Qun Ma
    • 1
  • Ji-Tao Du
    • 1
  • Sheng-Quan Zou
    • 1
  1. 1.Department of General Surgery, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan, HubeiChina

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