Annals of Surgical Oncology

, Volume 10, Issue 3, pp 297–304 | Cite as

Quantitative Analysis of Heparanase Gene Expression in Esophageal Squamous Cell Carcinoma

  • Masahide Ikeguchi
  • Kenji Fukuda
  • Ken-ichi Yamaguchi
  • Akira Kondo
  • Shun-ichi Tsujitani
  • Nobuaki Kaibara
Original Articles


Background: Heparan sulfate proteoglycans, the main components of the extracellular matrix, are recognized as important components of signal transduction and play an important role in tumor progression. Heparanase (hep) degrades heparan sulfate proteoglycans, but the clinical importance of hep is unclear. In this study, we investigated the clinicopathologic importance of hep messenger RNA (mRNA) expression in esophageal squamous cell carcinoma (ESCC).

Methods: Fresh tumors and noncancerous epithelia were obtained from 57 ESCC patients after esophagectomy. Expression levels of hep and glyceraldehyde-3-phosphate dehydrogenase mRNA were quantitatively analyzed by real-time reverse transcriptase-polymerase chain reaction. Apoptotic cancer cells and microvessel density were evaluated immunohistochemically.

Results: The relative hep mRNA expression level (hep:glyceraldehyde-3-phosphate dehydrogenase ratio) in ESCC was lower than in noncancerous tissue (P < .001). Tumor hep expression decreased according to tumor progression and correlated with the occurrence of apoptotic cancer cells, but not with tumor microvessel density. Moreover, low hep expression correlated with poor patient survival.

Conclusions: Reduced hep mRNA expression might result in abnormal cell growth and correlate with ESCC progression.

Key Words

Apoptosis Esophageal squamous cell carcinoma Heparan sulfate proteoglycans Heparanase Real-time reverse transcriptase-polymerase chain reaction 


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Copyright information

© The Society of Surgical Oncology, Inc. 2003

Authors and Affiliations

  • Masahide Ikeguchi
    • 1
    • 3
  • Kenji Fukuda
    • 2
  • Ken-ichi Yamaguchi
    • 2
  • Akira Kondo
    • 2
  • Shun-ichi Tsujitani
    • 2
  • Nobuaki Kaibara
    • 2
  1. 1.Division of Operating RoomFaculty of Medicine, Tottori UniversityYonagoJapan
  2. 2.Division of Surgical OncologyFaculty of Medicine, Tottori UniversityYonagoJapan
  3. 3.Division of Operating RoomFaculty of Medicine, Tottori UniversityJapan

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