Dentifrice Based on Fluoride–Hydrotalcite Compounds: Characterization and Release Capacity Evaluation by Novel In Vitro Methods


Anti-caries activity of fluoride ions is due to the protection against demineralization and the enhancement of remineralization of tooth enamel. Dentifrices available on the market contain sodium fluoride, sodium monofluorophosphate, stannous fluoride, and amine fluoride as source of these ions. A new compound working both as fluoride ion source and as abrasive was projected. Hybrids based on F ions intercalated between the lamellae of hydrotalcite-like compounds (HTlc-F), namely MgAl-HTlc-F and ZnAl-HTlc-F, were prepared and characterized. Then, three different percentages (2, 3, and 4%) of both HTlc-F compounds were assayed. After the rheological characterization, the dentifrices containing 3 and 4% of MgAl-HTlc-F and ZnAl-HTlc-F, respectively, resulted to be the most suitable ones. Two novel in vitro methods, “rotary toothbrush method” and “manual brushing method,” were developed and used in order to study the F ions release from the prepared dentifrices. The obtained results showed that the dentifrice containing ZnAl-HTlc-F (4%) was the most effective in releasing fluoride ions. The “rotary toothbrush method” resulted to be the most suitable as the simulation of the brushing movements is standardizable and reproducible.

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The authors are very grateful to Mr. Marco Marani for technical assistance.

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Correspondence to Luana Perioli.

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Electronic Supplementary Material


Figure 1SI. Thermogravimetric curves obtained from A) MgAl-HTlc-F and B) ZnAl-HTlc-F (PNG 561 kb)


Figure 2SI. XRPD patterns of dentifrices F (containing ZnAl-HTlc-F 4%, red line) and H (containing MgAl-HTlc-F 3% blue line) (PNG 87 kb)

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High resolution image (TIF 77 kb)

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Pagano, C., Perioli, L., Marmottini, F. et al. Dentifrice Based on Fluoride–Hydrotalcite Compounds: Characterization and Release Capacity Evaluation by Novel In Vitro Methods. AAPS PharmSciTech 20, 248 (2019).

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  • hydrotalcite
  • HTlc-F
  • abrasive
  • dentifrice
  • rheology
  • in vitro release method