Estimation of Anti-inflammatory and Analgesic Effects of Topical NANOCEN (Nanoliposomal Arthrocen) on Mice


The purpose of this study was to evaluate the effect of topical application of nanoliposomal avocado/soybean unsaponifiables (NANOCEN) on inflammation inhibition and pain relief in mice. NANOCEN was prepared by the injection method and characterized for vesicle size, charge, entrapment efficiency, in vitro release, and 1-month vesicle stability. The analysis of ASU formulation showed that liposomes had an average size of around 146 nm with a surface charge of − 43 mV. SEM and TEM imaging confirmed the spherical shape of the nanovesicles in ASU formulation. Moreover, ASU nanoliposomes had a high entrapment efficiency (96%) and exhibited significantly (p < 0.0001) sustained release of the drug in vitro model. The topical NANOCEN (ASU 2%) showed robust anti-inflammatory (p < 0.01) and analgesic effect (p < 0.01) superior to ibuprofen 5%. The histopathology of the inflamed tissues confirmed that the topical ASU formulation potentially (p < 0.001) inhibited infiltration of inflammatory cells. Our findings suggest that the topical formulation of NANOCEN may have local applications for pain relief in medicine.

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This research has been supported by Tehran University of Medical Sciences and Health Services grant (no. 96-04-158-37121).

Author information




Conceptualization: Ramin Goudarzi

Data curation: Alireza Partoazar, Sima Amini

Formal analysis: Alireza Partoazar

Funding acquisition: Tehran University of Medical Sciences

Methodology: Alireza Partoazar, Sima Amini

Project administration: Ramin Goudarzi

Resources: Ramin Goudarzi, Alireza Partoazar

Supervision: Alireza Partoazar

Validation: Ahmad Reza Dehpour

Writing—original draft: Ramin Goudarzi, Sima Amini

Corresponding author

Correspondence to Alireza Partoazar.

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Conflict of Interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article. Dr. Ramin Goudarzi owns equity in Pharmin USA which manufactures and distributes Arthrocen.

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Goudarzi, R., Amini, S., Dehpour, A.R. et al. Estimation of Anti-inflammatory and Analgesic Effects of Topical NANOCEN (Nanoliposomal Arthrocen) on Mice. AAPS PharmSciTech 20, 233 (2019).

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  • Inflammation
  • Nanoliposome
  • Osteoarthritis
  • Pain
  • Treatment
  • Arthrocen
  • Avocado soy unsaponifiables