AAPS PharmSciTech

, 20:53 | Cite as

Antioxidant Potential and Angiotensin-Converting Enzyme (ACE) Inhibitory Activity of Orotic Acid-Loaded Gum Arabic Nanoparticles

  • Abdelkader Hassani
  • Siti Aslina HussainEmail author
  • Norhafizah Abdullah
  • Suryani Kamarudin
  • Rozita Rosli
Research Article Theme: Translational Multi-Disciplinary Approach for the Drug and Gene Delivery Systems
Part of the following topical collections:
  1. Theme: Translational Multi-Disciplinary Approach for the Drug and Gene Delivery Systems


Orotic acid (OA) nanoparticles were prepared using the freeze-drying method. The antihypertensive activity and antioxidant capacity of OA and orotic acid-loaded gum arabic nanoparticles (OAGANPs) were examined using the angiotensin-converting enzyme (ACE), 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide (NO), and β-carotene assays, as well as the quantification of total phenolic content (TPC). The DPPH and NO scavenging activities of OAGANPs were significantly higher than those of the OA solution. The β-carotene bleaching assay of OAGANPs showed a dose-dependent trend, while 500 μg/ml was significantly more effective than the other concentrations, which exerted 63.4% of the antioxidant activity. The in vitro antihypertensive assay revealed that the OAGANPs exhibited the most potent ACE inhibition activity, when compared to the OA solution. Hence, results revealed the potential of preparing the OA as a nanoparticle formulation in enhancing the antioxidant and antihypertensive properties compared to the OA solution.


antioxidant antihypertensive nanoparticles radical scavenging 



The authors would like to acknowledge the Universiti Putra Malaysia and Department of Chemical and Environmental Engineering for awarding the funds to conduct this research under project number GP-IPS/2016/9505500.

Compliance with Ethical Standards

Conflicts of Interest

The authors declare that there are no conflicts of interest.


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Copyright information

© American Association of Pharmaceutical Scientists 2019

Authors and Affiliations

  1. 1.Department of Chemical and Environmental Engineering, Faculty of EngineeringUniversiti Putra MalaysiaSerdang, UPMMalaysia
  2. 2.UPM-MAKNA Cancer Research Laboratory, Institute of BioscienceUniversiti Putra MalaysiaSerdang, UPMMalaysia

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