AAPS PharmSciTech

, Volume 19, Issue 4, pp 1894–1907 | Cite as

Chitosan Nanoparticles of Gamma-Oryzanol: Formulation, Optimization, and In vivo Evaluation of Anti-hyperlipidemic Activity

  • Tejal Rawal
  • Neha Mishra
  • Abhishek Jha
  • Apurva Bhatt
  • Rajeev K. Tyagi
  • Shital Panchal
  • Shital Butani
Research Article


The elevated blood levels of cholesterol and low-density lipoproteins result in hyperlipidemia. The available expensive prophylactic treatments are kindred with severe side effects. Therefore, we fabricated the polymeric nanoparticles of gamma-oryzanol to achieving the improved efficacy of drug. The nanoparticles were prepared by ionic gelation method and optimized using 23 full factorial design taking drug/polymer ratio (X1), polymer/cross linking agent ratio (X2), and stirring speed (X3) as independent variables. The average particle size, percentage entrapment efficiency, and in vitro drug release at 2, 12, and 24 h were selected as response parameters. The factorial batches were statistically analyzed and optimized. The optimized nanoparticles were characterized with respect to particle size (141 nm) and zeta potential (+ 6.45 mV). Results obtained with the prepared and characterized formulation showed 83% mucoadhesion towards the intestinal mucosa. The in vitro findings were complemented well by in vivo anti-hyperlipidemic activity of developed formulation carried out in Swiss albino mouse model. The in vivo studies showed improved atherogenic index, malondialdehyde, and superoxide dismutase levels in poloxamer-407-induced hyperlipidemic animals when treated with oryzanol and gamma-oryzanol nanoformulation. Based on our findings, we believe that chitosan-mediated delivery of gamma-oryzanol nanoparticles might prove better in terms of anti-hyperlipidemic therapeutics.


gamma-oryzanol chitosan nanoparticles poloxamer-407 atorvastatin 



The authors would like to thank Institute of Pharmacy, Nirma University, Ahmedabad for providing us all the facilities to carry out our research work.

Compliance with Ethical Standards

The animal protocol was approved by the Institutional Animal Ethics Committee (Protocol no. IP/PCEU/MPH/14-1/010). All animals were treated in accordance with the guidelines of the committee for CPCSEA.

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© American Association of Pharmaceutical Scientists 2018

Authors and Affiliations

  • Tejal Rawal
    • 1
  • Neha Mishra
    • 1
  • Abhishek Jha
    • 1
  • Apurva Bhatt
    • 1
  • Rajeev K. Tyagi
    • 2
    • 3
  • Shital Panchal
    • 1
  • Shital Butani
    • 1
  1. 1.Department of Pharmaceutical Technology, Institute of PharmacyNirma UniversityAhmedabadIndia
  2. 2.Institute of ScienceNirma UniversityAhmedabadIndia
  3. 3.Department of Periodontics, College of Dental MedicineGeorgia Regents UniversityAugustaUSA

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