AAPS PharmSciTech

, Volume 11, Issue 4, pp 1675–1683 | Cite as

Porous Bioactive Glass Scaffolds for Local Drug Delivery in Osteomyelitis: Development and In Vitro Characterization

  • Chidambaram Soundrapandian
  • Someswar Datta
  • Biswanath Kundu
  • Debabrata Basu
  • Biswanath Sa
Research Article


A new bioactive glass-based scaffold was developed for local delivery of drugs in case of osteomyelitis. Bioactive glass having a new composition was prepared and converted into porous scaffold. The bioactivity of the resulting scaffold was examined by in vitro acellular method. The scaffolds were loaded with two different drugs, an antibacterial or antifungal drug. The effects of the size of the scaffold, drug concentration, and dissolution medium on drug release were studied. The scaffolds were further coated with a degradable natural polymer, chitosan, to further control the drug release. Both the glass and scaffold were bioactive. The scaffolds released both the drugs for 6 weeks, in vitro. The results indicated that the bigger the size and the higher the drug concentration, the better was the release profile. The scaffolds appeared to be suitable for local delivery of the drugs in cases of osteomyelitis.

Key words

bioceramics bone infection drug release organic–inorganic composite skeletal drug delivery 



The authors wish to express their sincere thanks to The Director, CG&CRI, India for the kind permission and support for the successful completion of the research work. All the departments and technical staffs involved in characterization are duly acknowledged.


  1. 1.
    Soundrapandian C, Datta S, Sa B. Drug-eluting implants for osteomyelitis. Crit Rev Ther Drug Carrier Syst. 2007;24:493–545.PubMedGoogle Scholar
  2. 2.
    Soundrapandian C, Sa B, Datta S. Organic–inorganic composites for bone drug delivery. AAPS PharmSciTech. 2009;10:1158–71.CrossRefPubMedGoogle Scholar
  3. 3.
    Nandi SK, Mukherjee P, Roy S, Kundu B, De DK, Basu D. Local antibiotic delivery systems for the treatment of osteomyelitis—a review. Mat Sci Eng C. 2009;29:2478–85.CrossRefGoogle Scholar
  4. 4.
    Ouedraogo M, Semde R, Some IT, Traore-Ouedraogo R, Guissou IP, Henschel V, et al. Monoolein–water liquid crystalline gels of gentamicin as bioresorbable implants for the local treatment of chronic osteomyelitis: in vitro characterization. Drug Dev Ind Pharm. 2008;34:753–60.CrossRefPubMedGoogle Scholar
  5. 5.
    Kundu B, Soundrapandian C, Nandi SK, Mukherjee P, Dandapat N, Roy S, et al. Development of new localized drug delivery system based on ceftriaxone–sulbactam composite drug impregnated porous hydroxyapatite: a systematic approach for in vitro and in vivo animal trial. Pharm Res. 2010;27:1659–76.CrossRefPubMedGoogle Scholar
  6. 6.
    Balamurugan A, Balossier G, Kannan S, Michel J, Rebelo AH, Ferreira JM. Development and in vitro characterization of sol–gel derived CaO–P2O5–SiO2–ZnO bioglass. Acta Biomater. 2007;3:255–62.CrossRefPubMedGoogle Scholar
  7. 7.
    Balamurugan A, Balossier G, Laurent-Maquin D, Pina S, Rebelo AH, Faure J, et al. An in vitro biological and anti-bacterial study on a sol–gel derived silver-incorporated bioglass system. Dent Mater. 2008;24:1343–51.CrossRefPubMedGoogle Scholar
  8. 8.
    Zhu Y, Kaskel S. Comparison of the in vitro bioactivity and drug release property of mesoporous bioactive glasses (MBGs) and bioactive glasses (BGs) scaffolds. Micropor Mesopor Mat. 2009;118:176–82.CrossRefGoogle Scholar
  9. 9.
    Czarnobaj K. Preparation and characterization of silica xerogels as carriers for drugs. Drug Deliv. 2008;15:485–92.CrossRefPubMedGoogle Scholar
  10. 10.
    Agathopoulos S, Tulyaganov DU, Ventura JMG, Kannan S, Saranti A, Karakassides MA, et al. Structural analysis and devitrification of glasses based on the CaO–MgO–SiO2 system with B2O3, Na2O, CaF2 and P2O5 additives. J Non-Cryst Solids. 2006;352:322–8.CrossRefGoogle Scholar
  11. 11.
    Bang H-G, Kim S-J, Park S-Y. Biocompatibility and the physical properties of bio-glass ceramics in the Na2O–CaO–SiO2–P2O5 system with CaF2 and MgF2 additives. J Ceram Process Res. 2008;9:588–90.Google Scholar
  12. 12.
    Radev L, Hristov V, Samuneva B, Ivanova D. Organic/inorganic bioactive materials. Part II: in vitro bioactivity of collagen-calcium phosphate silicate/wollastonite hybrids. Cent Eur J Chem. 2009;7:711–20.CrossRefGoogle Scholar
  13. 13.
    Nandi SK, Kundu B, Mukherjee P, Mandal TK, Datta S, De DK, et al. In vitro and in vivo release of cefuroxime axetil from bioactive glass as an implantable delivery system in experimental osteomyelitis. Ceram Int. 2009;35:3207–16.CrossRefGoogle Scholar
  14. 14.
    Kontonasaki E, Zorba T, Papadopoulou L, Pavlidou E, Chatzistavrou X, Paraskevopoulos K, et al. Hydroxy carbonate apatite formation on particulate bioglass in vitro as a function of time. Cryst Res Technol. 2002;37:1165–71.CrossRefGoogle Scholar
  15. 15.
    Hing KA. Bioceramic bone graft substitutes: influence of porosity and chemistry. Int J Appl Ceram Technol. 2005;2:184–99.CrossRefGoogle Scholar
  16. 16.
    Chai F, Hornez JC, Blanchemain N, Neut C, Descamps M, Hildebrand HF. Antibacterial activation of hydroxyapatite (HA) with controlled porosity by different antibiotics. Biomol Eng. 2007;24:510–4.CrossRefPubMedGoogle Scholar
  17. 17.
    Seeley Z, Bandyopadhyay A, Bose S. Tricalcium phosphate based resorbable ceramics: influence of NaF and CaO addition. Mat Sci Eng C. 2008;28:11–7.CrossRefGoogle Scholar
  18. 18.
    Orefice R, West J, LaTorre G, Hench L, Brennan A. Effect of long-term in vitro testing on the properties of bioactive glass–polysulfone composites. Biomacromolecules. 2010;11:657–65.CrossRefPubMedGoogle Scholar
  19. 19.
    Balamurugan A, Balossier G, Michel J, Kannan S, Benhayoune H, Rebelo AH, et al. Sol gel derived SiO(2)–CaO–MgO–P(2)O(5) bioglass system—preparation and in vitro characterization. J Biomed Mater Res B Appl Biomater. 2007;83:546–53.PubMedGoogle Scholar
  20. 20.
    NCCLS. Performance standards for antimicrobial susceptibility testing. Wayne: NCCLS; 2002.Google Scholar
  21. 21.
    Xia W, Chang J. Well-ordered mesoporous bioactive glasses (MBG): a promising bioactive drug delivery system. J Control Release. 2006;110:522–30.CrossRefPubMedGoogle Scholar
  22. 22.
    Noble L, Gray AI, Sadiq L, Uchegbu IF. A non-covalently cross-linked chitosan based hydrogel. Int J Pharm. 1999;192:173–82.CrossRefPubMedGoogle Scholar
  23. 23.
    Rossi S, Marciello M, Sandri G, Bonferoni MC, Ferrari F, Caramella C. Chitosan ascorbate: a chitosan salt with improved penetration enhancement properties. Pharm Dev Tech. 2008;13:513–21.CrossRefGoogle Scholar
  24. 24.
    Ubaidulla U, Khar RK, Ahmad FJ, Tripathi P. Optimization of chitosan succinate and chitosan phthalate microspheres for oral delivery of insulin using response surface methodology. Pharm Dev Tech. 2009;14:96–105.CrossRefGoogle Scholar
  25. 25.
    Kong M, Chen XG, Liu CS, Liu CG, Meng XH, Yule J. Antibacterial mechanism of chitosan microspheres in a solid dispersing system against E. coli. Colloids Surf B: Biointerfaces. 2008;65:197–202.CrossRefGoogle Scholar
  26. 26.
    Park Y, Kim MH, Park SC, Cheong H, Jang MK, Nah JW, et al. Investigation of the antifungal activity and mechanism of action of LMWS-chitosan. J Microbiol Biotechnol. 2008;18:1729–34.PubMedGoogle Scholar
  27. 27.
    Baldrick P. The safety of chitosan as a pharmaceutical excipient. Regul Toxicol Pharmacol. 2010;56:290–9.CrossRefPubMedGoogle Scholar
  28. 28.
    El-Kamel AH, Baddour MM. Gatifloxacin biodegradable implant for treatment of experimental osteomyelitis: in vitro and in vivo evaluation. Drug Deliv. 2007;14:349–56.CrossRefPubMedGoogle Scholar
  29. 29.
    Melville A, Rodríguez-Lorenzo L, Forsythe J. Effects of calcination temperature on the drug delivery behaviour of ibuprofen from hydroxyapatite powders. J Mater Sci Mater Med. 2008;19:1187–95.CrossRefPubMedGoogle Scholar

Copyright information

© American Association of Pharmaceutical Scientists 2010

Authors and Affiliations

  • Chidambaram Soundrapandian
    • 1
    • 2
  • Someswar Datta
    • 1
  • Biswanath Kundu
    • 1
  • Debabrata Basu
    • 1
  • Biswanath Sa
    • 2
  1. 1.Bioceramic and Coating DivisionCentral Glass & Ceramic Research InstituteKolkataIndia
  2. 2.Department of Pharmaceutical TechnologyJadavpur UniversityKolkataIndia

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