AAPS PharmSciTech

, Volume 11, Issue 3, pp 1376–1382 | Cite as

Polyethylene Glycol on Stability of Chitosan Microparticulate Carrier for Protein

  • Manee Luangtana-anan
  • Sontaya Limmatvapirat
  • Jurairat Nunthanid
  • Rapeepun Chalongsuk
  • Keiji Yamamoto
Research Article


Stability enhancement of protein-loaded chitosan microparticles under storage was investigated. Chitosan glutamate at 35 kDa and bovine serum albumin as model protein drug were used in this study. The chitosan microparticles were prepared by ionotropic gelation, and polyethylene glycol 200 (PEG 200) was applied after the formation of the particles. All chitosan microparticles were kept at 25°C for 28 days. A comparison was made between those preparations with PEG 200 and without PEG 200. The changes in the physicochemical properties of the microparticles such as size, zeta potential, pH, and percent loading capacity were investigated after 0, 3, 7, 14, and 28 days of storage. It was found that the stability decreased upon storage and the aggregation of microparticles could be observed for both preparations. The reduction in the zeta potential and the increase in the pH, size, and loading capacity were observed when they were kept at a longer period. The significant change of those preparations without PEG 200 was evident after 7 days of storage whereas those with PEG 200 underwent smaller changes with enhanced stability after 28 days of storage. Therefore, this investigation gave valuable information on the stability enhancement of the microparticles. Hence, enhanced stability of chitosan glutamate microparticles for the delivery of protein could be achieved by the application of PEG 200.

Key words

chitosan microparticles polyethylene glycol 200 protein stability 



The author wishes to thank the Japan Society for the Promotion of Science (JSPS) for their financial support and the research and development institute, the University of Silpakorn, Thailand.


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Copyright information

© American Association of Pharmaceutical Scientists 2010

Authors and Affiliations

  • Manee Luangtana-anan
    • 1
    • 2
  • Sontaya Limmatvapirat
    • 1
    • 2
  • Jurairat Nunthanid
    • 1
    • 2
  • Rapeepun Chalongsuk
    • 3
  • Keiji Yamamoto
    • 4
  1. 1.Department of Pharmaceutical Technology, Faculty of PharmacySilpakorn UniversityNakhon PathomThailand
  2. 2.Pharmaceutical Biopolymer Group (PBiG), Faculty of PharmacySilpakorn UniversityNakhon PathomThailand
  3. 3.Department of Community Pharmacy, Faculty of PharmacySilpakorn UniversityNakorn PathomThailand
  4. 4.Department of Pharmaceutical Technology, Graduate School of Pharmaceutical SciencesChiba UniversityChibaJapan

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