AAPS PharmSciTech

, Volume 11, Issue 3, pp 1243–1249 | Cite as

Formulation of Dacarbazine-loaded Cubosomes. Part III. Physicochemical Characterization

  • Di Bei
  • Tao Zhang
  • James B. Murowchick
  • Bi-Botti C. Youan
Research Article

Abstract

The purpose of this study was to investigate the physicochemical properties of dacarbazine-loaded cubosomes. The drug-loaded cubosome nanocarriers were prepared by a fragmentation method and then freeze dried. They were then characterized for size, morphology, thermal behavior, and crystallography using dynamic light scattering, transmission electron microscopy (TEM), differential scanning calorimetry (DSC), and powder X-ray diffraction (PXRD), respectively. The drug loading and encapsulation efficiency were determined by UV spectrophotometry. The results showed that the prepared dacarbazine-loaded cubosomes had mean diameters ranging from 86 to 106 nm. In addition to the TEM, the characteristic peaks from PXRD data suggested that the freeze-dried nanoformulations were indeed cubic in nature. DSC and PXRD analysis suggested the 0.06 or 0.28% w/w actual drug loaded inside cubosomes was in the amorphous or molecular state. These physicochemical characteristics would affect the nanoformulation shelf-life, efficacy, and safety.

Key words

cubosome dacarbazine thermal analysis X-ray diffraction 

Notes

ACKNOWLEDGMENTS

Monoolein (GMO) was kindly provided to us by Danisco Cultor (Grindsted, Denmark). We also appreciated the guidance of Dr. Elizabeth Kostoryz (Division of Pharmacology, University of Missouri–Kansas City) for the DLS experiment and the support of Randy Tindall (Electron Microscopy Center, University of Missouri–Columbia) for the electron microscopy. The author acknowledge the helpful and thorough proof reading of this manuscript by Margaret LoGiudice, R.D.H, M.S. (Johnson County Community College, Overland Park, KS)

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Copyright information

© American Association of Pharmaceutical Scientists 2010

Authors and Affiliations

  • Di Bei
    • 1
  • Tao Zhang
    • 1
  • James B. Murowchick
    • 2
  • Bi-Botti C. Youan
    • 1
  1. 1.Laboratory of Future Nanomedicines and Theoretical Chronopharmaceutics, Division of Pharmaceutical SciencesUniversity of Missouri-Kansas City School of PharmacyKansas CityUSA
  2. 2.Department of GeosciencesUniversity of MissouriKansas CityUSA

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