Abstract
Huge variation of drug transporter abundance was seen in the literature, making PBPK prediction difficult when transporters play a major role. Among multiple factors such as membrane fraction, digestion, and peptide selection that contributed to such variation, peptide selection is the least discussed. Herein, a strategy was established by using a small amount of purified protein standard to select a peptide with near 100% digestion efficiency for quantitation of a transporter protein MDR1. The impact of native membrane protein’s tertiary structure on the digestion efficiency of surrogate peptides of MDR1 was investigated. Peptides in more solvent accessible regions are found to be digested much more efficiently than those in large stretches of helical structures. The concentration of peptide EALDESIPPVSFWR(EAL) in the most solvent accessible linker region of MDR1 was found closest to the true protein concentration. When using EAL for MDR1 quantitation, the abundance is over 10 times higher than previously reported, indicating the importance of peptide selection for transporter quantitation. In addition, this study also proposes a screening strategy to select peptides appropriate for relative quantitation for in vitro-in vivo extrapolation in the absence of any protein standard.
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06 June 2018
Liling Liu was not noted as the co-first author in the original article. Buyun Chen and Liling Liu contributed equally to the article.
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Acknowledgements
The authors thank Dr. Kebing Yu and Dr. Donald Kirkpatrick for sharing their experience of digestion bias in shot-gun proteomics.
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Chen, B., Liu, L., Ho, H. et al. Strategies of Drug Transporter Quantitation by LC-MS: Importance of Peptide Selection and Digestion Efficiency. AAPS J 19, 1469–1478 (2017). https://doi.org/10.1208/s12248-017-0106-4
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DOI: https://doi.org/10.1208/s12248-017-0106-4