The AAPS Journal

, Volume 16, Issue 4, pp 872–874 | Cite as

The Gender of Cell Lines Matters When Screening for Novel Anti-Cancer Drugs

  • Larissa M. Nunes
  • Elisa Robles-Escajeda
  • Yahaira Santiago-Vazquez
  • Nora M. Ortega
  • Carolina Lema
  • Almendra Muro
  • Gladys Almodovar
  • Umashankar Das
  • Swagatika Das
  • Johnatan R. Dimmock
  • Renato J. Aguilera
  • Armando Varela-Ramirez
Brief/Technical Note

Abstract

Current reports indicated that the gender origin of cells is important in all facets of experimental biology. To explore this matter using an anticancer high throughput screening platform, seven male- and seven female-derived human cell lines, six from cancer patients in each group, were exposed to 81 novel cytotoxins. In this screen, the findings revealed that 79 out of 81 of the compounds consistently inflicted higher levels of toxicity towards male derived cells, emphasizing that there is indeed a gender-related difference in cell sensitivity to these anti-neoplastic agents. This gender-related drug sensitivity and toxicity explored at the molecular and cellular level emerged from a drug discovery enterprise.

KEY WORDS

cancer cytotoxicity drug discovery high throughput screening 

Notes

Acknowledgments

Funding for this work was provided by the NIGMS SCORE grant 1SC3GM103713-01 to R.J.A., as well as a CIHR-RPP Saskatchewan grant to J.R.D and U.D. We thank the Cytometry, Screening and Imaging Core Facility at the University of Texas at El Paso (UTEP) which was supported by a RCMI program grant 8G12MD007592 to the BBRC-UTEP from the NIMHD-NIH. E.R-E, Y.S-V and N.M.O. were supported by NIGMS RISE training grant R25 GM069621-10. N.M.O. was also supported by MARC U*STAR program grant 2T34GM008048.

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Copyright information

© American Association of Pharmaceutical Scientists 2014

Authors and Affiliations

  • Larissa M. Nunes
    • 1
  • Elisa Robles-Escajeda
    • 1
  • Yahaira Santiago-Vazquez
    • 1
  • Nora M. Ortega
    • 1
  • Carolina Lema
    • 1
  • Almendra Muro
    • 1
  • Gladys Almodovar
    • 1
  • Umashankar Das
    • 2
  • Swagatika Das
    • 2
  • Johnatan R. Dimmock
    • 2
  • Renato J. Aguilera
    • 1
  • Armando Varela-Ramirez
    • 1
  1. 1.Cytometry, Screening and Imaging Core Facility & Border Biomedical Research Center & Department of Biological SciencesUniversity of Texas at El PasoEl PasoUSA
  2. 2.Drug Discovery and Development Research Group, College of Pharmacy & NutritionUniversity of SaskatchewanSaskatoonCanada

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