The AAPS Journal

, 11:465 | Cite as

Development of an In Vivo Ovine Dry Powder Inhalation Model for the Evaluation of Conventional and Controlled Release Microparticles

  • Rania O. Salama
  • Leigh Ladd
  • Hak-Kim Chan
  • Daniela Traini
  • Paul M. Young
Brief/Technical Note

Key words

controlled release dry powder inhalation in vivo ovine model pharmacokinetics 



This work has been supported by the Clive and Vera Ramaciotti Foundations establishment grant. The authors would like to thank Prof. Andrew McLachlan for his advice and guidance in interpreting the PK data. We would also like to thank the animal house’s manager and staff at Royal North Shore Hospital for their help and assistance during the experiments.

The scholarship for RS from the Egyptian government is acknowledged.


  1. 1.
    Murakami K, Bjertnaes LJ, Schmalstieg FC, McGuire R, Cox RA, Hawkins HK, et al. A novel animal model of sepsis after acute lung injury in sheep. Crit Care Med. 2002;30:2083–90.PubMedCrossRefGoogle Scholar
  2. 2.
    Abraham WM. Modeling of asthma, COPD and cystic fibrosis in sheep. Pulm Pharmacol Ther. 2008;21:743–54.PubMedCrossRefGoogle Scholar
  3. 3.
    Begin R, Rola-Pleszczynski M, Sirois P, Masse S, Nadeau D, Bureau MA. Sequential analysis of the bronchoalveolar milieu in conscious sheep. J Appl Physiol. 1981;50:665–71.PubMedGoogle Scholar
  4. 4.
    Bischof RJ, Snibson K, Shaw R, Meeusen ENT. Induction of allergic inflammation in the lungs of sensitized sheep after local challenge with house dust mite. Clin Exp Allergy. 2003;33:367–75.PubMedCrossRefGoogle Scholar
  5. 5.
    Salama R, Hoe S, Chan H-K, Traini D, Young PM. Preparation and characterisation of controlled release co-spray dried drug-polymer microparticles for inhalation 1: influence of polymer concentration on physical and in vitro characteristics. Eur J Pharm Biopharm. 2008;69:486–95.PubMedCrossRefGoogle Scholar
  6. 6.
    Salama RO, Traini D, Chan H-K, Young PM. Preparation and characterisation of controlled release co-spray dried drug-polymer microparticles for inhalation 2: Evaluation of in vitro release profiling methodologies for controlled release respiratory aerosols. Eur J Pharm Biopharm. 2008;70:145–52.PubMedCrossRefGoogle Scholar
  7. 7.
    Tang P, Fletcher DF, Chan HK, Raper JA. Simple and cost-effective powder disperser for aerosol particle size measurement. Powder Technol. 2008;187:27–36.CrossRefGoogle Scholar
  8. 8.
    Kato Y, Muraki K, Fujitaka M, Sakura N, Ueda K. Plasma concentrations of disodium cromoglycate after various inhalation methods in healthy subjects. Br J Clin Pharmacol. 1999;48:154–7.PubMedCrossRefGoogle Scholar
  9. 9.
    Ozoux ML, Girault J, Malgouyat JM, Pasquier O. Determination of sodium cromoglycate in human plasma by liquid chromatography–mass spectrometry in the turbo ion spray mode. J Chromatogr B Biomed Sci Appl. 2001;765:179–85.CrossRefGoogle Scholar
  10. 10.
    Moss GF, Jones KM, Ritchie JT, Cox JSG. Plasma levels and urinary excretion of disodium cromoglycate after inhalation by human volunteers. Toxicol Appl Pharmacol. 1971;20:147–56.PubMedCrossRefGoogle Scholar
  11. 11.
    Higuchi T. J Soc Cosmet Chem. 1960;11:85–97.Google Scholar
  12. 12.
    Higuchi T. Rate of release of medicaments from ointment bases containing drugs in suspension. J Pharm Sci. 1961;50:874–5.PubMedCrossRefGoogle Scholar
  13. 13.
    Yamamoto A, Yamada K, Muramatsu H, Nishinaka A, Okumura S, Okada N, et al. Control of pulmonary absorption of water-soluble compounds by various viscous vehicles. Int J Pharm. 2004;282:141–9.PubMedCrossRefGoogle Scholar
  14. 14.
    Moss GF, Ritchie JT. Absorption and clearance of disodium cromoglycate from lung in rat, rabbit, and monkey. Toxicol Appl Pharmacol. 1970;17:699–707.PubMedCrossRefGoogle Scholar
  15. 15.
    Martindale: the complete drug reference. London: The Pharmaceutical Press; 2008.Google Scholar

Copyright information

© American Association of Pharmaceutical Scientists 2009

Authors and Affiliations

  • Rania O. Salama
    • 1
  • Leigh Ladd
    • 2
  • Hak-Kim Chan
    • 1
  • Daniela Traini
    • 1
  • Paul M. Young
    • 1
  1. 1.Advanced Drug Delivery Group, Faculty of PharmacyUniversity of SydneySydneyAustralia
  2. 2.School of Animal and Veterinary SciencesCharles Stuart UniversityWagga WaggaAustralia

Personalised recommendations