The AAPS Journal

, Volume 8, Issue 4, pp E743–E755 | Cite as

Metabolism of GTI-2040, a phosphorothioate oligonucleotide antisense, using ion-pair reversed phase high performance liquid chromatography (HPLC) coupled with electrospray ion-trap mass spectrometry

  • Xiaohui Wei
  • Guowei Dai
  • Zhongfa Liu
  • Hao Cheng
  • Zhiliang Xie
  • Guido Marcucci
  • Kenneth K. Chan
Article

Abstract

GTI-2040 is a 20-mer phosphorothioate oligonucleotide, which is complementary to the messenger ribonucleic acid (mRNA) of the R2 subunit of ribonucleotide reductase. This study characterized both the in vivo and in vitro metabolism of GTI-2040. A highly specific ion-pair reversed-phase electrospray ionization (IP-RP-ESI) liquid chromatographymass spectrometry (LC-MS) method was used for the identification of GTI-2040 and metabolites from a variety of biological samples including exonuclease enzyme solutions, plasma, urine, mouse liver/kidney homogenates, and human liver microsomes. Progressively chain-shortened metabolites trucated from the 3′ terminal of GTI-2040 were detected in all of the evaluated biological samples. GTI-2040 was found to be a good substrate for 3′ but not 5′ exonuclease. While the pattern of n-1 chain-shortened 3′-exonucleolytic degradation was similar in the mouse liver and kidney homogenates, the latter was found to contain a larger number of shortenmers, the kidneys appeared to possess higher enzymatic reactivity toward GTI-2040. Thus, metabolism of GTI-2040 was found to occur in a variety of biological samples, mainly mediated by the 3′ exonuclease.

Keywords

Metabolism phosphorothioate oligonucleotides GTI-2040 liquid chromatography/mass spectrometry 

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Copyright information

© American Association of Pharmaceutical Scientists 2006

Authors and Affiliations

  • Xiaohui Wei
    • 1
  • Guowei Dai
    • 2
  • Zhongfa Liu
    • 1
  • Hao Cheng
    • 1
  • Zhiliang Xie
    • 1
  • Guido Marcucci
    • 3
    • 4
    • 5
  • Kenneth K. Chan
    • 1
    • 3
  1. 1.Division of Pharmaceutics, College of PharmacyThe Ohio State UniversityColumbus
  2. 2.Pharmaceutical Research InstituteBristol-Myers-Squibb CoPrinceton
  3. 3.The Comprehensive Cancer CenterThe Ohio State UniversityColumbus
  4. 4.Division of Hematology-Oncology, College of MedicineThe Ohio State UniversityColumbus
  5. 5.Room 308 OSU CCCThe Ohio State UniversityColumbus

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