AAPS PharmSciTech

, 20:218 | Cite as

Enhancement of Oral Bioavailability and Anti-hyperuricemic Activity of Isoliquiritigenin via Self-Microemulsifying Drug Delivery System

  • Kangyi Zhang
  • Qilong Wang
  • Qiuxuan Yang
  • Qiuyu Wei
  • Na Man
  • Michael Adu-Frimpong
  • Elmurat Toreniyazov
  • Hao Ji
  • Jiangnan YuEmail author
  • Ximing XuEmail author
Research Article Theme: Lipid-Based Drug Delivery Strategies for Oral Drug Delivery
Part of the following topical collections:
  1. Theme: Lipid-Based Drug Delivery Strategies for Oral Drug Delivery


The aim of this study was to develop a self-microemulsifying drug delivery system (SMEDDS) for enhancement of the oral bioavailability of isoliquiritigenin (ISL) as well as evaluate its in vivo anti-hyperuricemic effect in rats. The ISL-loaded self-microemulsifying drug delivery system (ISL-SMEDDS) was comprised of ethyl oleate (EO, oil phase), Tween 80 (surfactant), and PEG 400 (co-surfactant). The ISL-SMEDDS exhibited an acceptable narrow size distribution (44.78 ± 0.35 nm), negative zeta potential (− 10.67 ± 0.86 mV), and high encapsulation efficiency (98.17 ± 0.24%). The in vitro release study indicated that the release rates of the formulation were obviously higher in different release media (HCl, pH 1.2; PBS, pH 6.8; double-distilled water, pH 7.0) compared with the ISL solution. The oral bioavailability of the ISL-SMEDDS was enhanced by 4.71 times in comparison with the free ISL solution. More importantly, ISL-SMEDDS significantly reduced uric acid level by inhibiting xanthine oxidase (XOD) activity in the model rats. Collectively, the prepared ISL-SMEDDS proved to be potential carriers for enhancing the solubility and oral bioavailability of ISL, as well as ameliorating its anti-hyperuricemic effect.

Key Words

Isoliquiritigenin ISL-SMEDDS bioavailability in vitro release anti-hyperuricemic 



The authors thank the Jiangsu University Ethics Committee for their kind guidance in the animal experiments.


This work was supported by the National Natural Science Foundation of China (81503025, 81473172, and 81773695), China Postdoctoral Science Foundation (2017M621658 and 2017M621659), and Special Funds for 331 and 333 projects (BRA2013198).

Compliance with Ethical Standards

The protocol for the experiment was in compliance with Jiangsu University’s Ethics Committee and guidelines spelt out for animal study by National Institute for Care and Use of Laboratory Animals (UJS-IACUC-2019032202).

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© American Association of Pharmaceutical Scientists 2019

Authors and Affiliations

  • Kangyi Zhang
    • 1
  • Qilong Wang
    • 1
  • Qiuxuan Yang
    • 1
  • Qiuyu Wei
    • 1
  • Na Man
    • 1
  • Michael Adu-Frimpong
    • 1
  • Elmurat Toreniyazov
    • 2
    • 3
  • Hao Ji
    • 2
    • 4
  • Jiangnan Yu
    • 1
    • 2
    • 5
    Email author
  • Ximing Xu
    • 1
    • 2
    • 5
    Email author
  1. 1.Department of Pharmaceutics, School of Pharmacy, Center for Nano Drug/Gene Delivery and Tissue EngineeringJiangsu UniversityZhenjiangPeople’s Republic of China
  2. 2.Jiangsu Provincial Research Center for Medicinal Function Development of New Food ResourcesZhenjiangPeople’s Republic of China
  3. 3.Tashkent State Agricultural University (Nukus Branch)NukusUzbekistan
  4. 4.Jiangsu Tian Sheng Pharmaceutical Co., LtdZhenjiangPeople’s Republic of China
  5. 5.Key Laboratory of Drug Delivery & Tissue RegenerationZhenjiangPeople’s Republic of China

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