An Evaluation of Curcumin-Encapsulated Chitosan Nanoparticles for Transdermal Delivery
- 24 Downloads
Curcumin-loaded chitosan nanoparticles were synthesised and evaluated in vitro for enhanced transdermal delivery. Zetasizer® characterisation of three different formulations of curcumin nanoparticles (Cu-NPs) showed the size ranged from 167.3 ± 3.8 nm to 251.5 ± 5.8 nm, the polydispersity index (PDI) values were between 0.26 and 0.46 and the zeta potential values were positive (+ 18.1 to + 20.2 mV). Scanning electron microscopy (SEM) images supported this size data and confirmed the spherical shape of the nanoparticles. All the formulations showed excellent entrapment efficiency above 80%. FTIR results demonstrate the interaction between chitosan and sodium tripolyphosphate (TPP) and confirm the presence of curcumin in the nanoparticle. Differential scanning calorimetry (DSC) studies of Cu-NPs indicate the presence of curcumin in a disordered crystalline or amorphous state, suggesting the interaction between the drug and the polymer. Drug release studies showed an improved drug release at pH 5.0 than in pH 7.4 and followed a zero order kinetics. The in vitro permeation studies through Strat-M® membrane demonstrated an enhanced permeation of Cu-NPs compared to aqueous curcumin solution (p ˂ 0.05) having a flux of 0.54 ± 0.03 μg cm−2 h−1 and 0.44 ± 0.03 μg cm−2 h−1 corresponding to formulations 5:1 and 3:1, respectively. The cytotoxicity assay on human keratinocyte (HaCat) cells showed enhanced percentage cell viability of Cu-NPs compared to curcumin solution. Cu-NPs developed in this study exhibit superior drug release and enhanced transdermal permeation of curcumin and superior percentage cell viability. Further ex vivo and in vivo evaluations will be conducted to support these findings.
KEY WORDStransdermal curcumin nanoparticles in vitro drug release permeation
The authors would like to acknowledge the Faculty of Science at the University of Nottingham Malaysia (UNM) for the financial support of this project.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 19.Ravikumar R, Ganesh M, Senthil V, Ramesh YV, Jakki SL, Choi EY. Tetrahydro curcumin loaded PCL-PEG electrospun transdermal nanofiber patch: preparation, characterization, and in vitro diffusion evaluations. J Drug Deliv Sci Technol. 2018;44:342–8. https://doi.org/10.1016/j.jddst.2018.01.016.CrossRefGoogle Scholar
- 20.Ravikumar R, Ganesh M, Ubaidulla U, Young Choi E, Tae Jang H. Preparation, characterization, and in vitro diffusion study of nonwoven electrospun nanofiber of curcumin-loaded cellulose acetate phthalate polymer. Saudi Pharm J. 2017;25:921–6. https://doi.org/10.1016/j.jsps.2017.02.004.CrossRefPubMedPubMedCentralGoogle Scholar
- 43.Rajesh S, Sujith S. Permeation of flurbiprofen polymeric films through human cadaver skin. Int J Pharm Tech Res. 2013;5:177–82.Google Scholar
- 66.Katas H, Hussain Z, Ling TC. Chitosan nanoparticles as a percutaneous drug delivery system for hydrocortisone. J Nanomater. 2012;2012:45.Google Scholar
- 71.Ratanajiajaroen P, Watthanaphanit A, Tamura H, Tokura S, Rujiravanit R. Release characteristic and stability of curcumin incorporated in β-chitin non-woven fibrous sheet using Tween 20 as an emulsifier. Eur Polym J. 2012;48:512–23. https://doi.org/10.1016/j.eurpolymj.2011.11.020.CrossRefGoogle Scholar
- 72.Doaa Nabih M, Sanjay RM, Lijia W, Abd-Elgawad Helmy A-E, Osama Abd-Elazeem S, Marwa Salah E-D, et al. Water-soluble complex of curcumin with cyclodextrins: enhanced physical properties for ocular drug delivery. Curr Drug Deliv. 2017;14:875–86. https://doi.org/10.2174/1567201813666160808111209.74.CrossRefGoogle Scholar