Bacterial Ghosts Carrying 5-Fluorouracil: A Novel Biological Carrier for Targeting Colorectal Cancer
- 109 Downloads
Bacterial ghosts (BGs) are non-deformed bacterial cell envelopes that possess undamaged external configurations for precise attachment to different cells of the human body. The Escherichia coli BGs were successfully produced using a modified sponge-like reduced protocol and characterized by SEM. Four different concentrations of 5-fluorouracil (5-FU) were used to study the impact on the “ghosts” cell wall. 5-FU was then loaded into the BGs and the loading capacity (LC %) and entrapment efficiency (EE %) were determined and were found to be 38.3 ± 0.8 and 76.6 ± 0.8, respectively. The in vitro release studies were conducted in dialysis bags over a time period of 16 days and the accumulative 5-FU released (%) was calculated. Overall, 69.2% of the ghost-associated 5-FU was released from the BGs and release from the E. coli ghosts is governed by non-Fickian diffusion. The Caco-2 cell line was used to investigate the cytotoxicity of 5-FU-loaded BGs.
KEY WORDStargeting 5-fluorouracil bacterial ghost colorectal cancer cytotoxicity
This project was financially supported by King Saud University, Vice Deanship of Research Chairs, Kayyali Chair for Pharmaceutical industry, through the Grant No. MS-2018.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 2.Joel GH, Lee EL, Alfred G. The pharmacological basis of therapeutics. International edition, 10th edition, Mc Grow Hill 2001, 971.Google Scholar
- 9.Sekar V. A rapid screening procedure for the identification of recombinant bacterial clones. BioTechniques. 1987;5(1):11–13.Google Scholar
- 22.Rahul KS, Kadam V, Shendarkar G, Jadhav S, Bharkad V. Sustained release drug delivery system: review. Ind J Res Pharm Biotechnol. 2011;3:246–51.Google Scholar
- 31.Stein E, Inic-Kanada A, Belij S, Montanaro J, Bintner N, Schlacher S, et al. In vitro and in vivo uptake study of Escherichia coli Nissle 1917 bacterial ghosts: cell-based delivery system to target ocular surface diseases Escherichia coli Nissle 1917 bacterial ghosts. Invest Ophthalmol Vis Sci. 2013;54:6326–33.CrossRefGoogle Scholar