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AAPS PharmSciTech

, 20:48 | Cite as

Bacterial Ghosts Carrying 5-Fluorouracil: A Novel Biological Carrier for Targeting Colorectal Cancer

  • Abdullah M. E. Youssof
  • Fars K. Alanazi
  • Mounir M. Salem-Bekhit
  • Faiyaz Shakeel
  • Nazrul HaqEmail author
Research Article
  • 14 Downloads

Abstract

Bacterial ghosts (BGs) are non-deformed bacterial cell envelopes that possess undamaged external configurations for precise attachment to different cells of the human body. The Escherichia coli BGs were successfully produced using a modified sponge-like reduced protocol and characterized by SEM. Four different concentrations of 5-fluorouracil (5-FU) were used to study the impact on the “ghosts” cell wall. 5-FU was then loaded into the BGs and the loading capacity (LC %) and entrapment efficiency (EE %) were determined and were found to be 38.3 ± 0.8 and 76.6 ± 0.8, respectively. The in vitro release studies were conducted in dialysis bags over a time period of 16 days and the accumulative 5-FU released (%) was calculated. Overall, 69.2% of the ghost-associated 5-FU was released from the BGs and release from the E. coli ghosts is governed by non-Fickian diffusion. The Caco-2 cell line was used to investigate the cytotoxicity of 5-FU-loaded BGs.

KEY WORDS

targeting 5-fluorouracil bacterial ghost colorectal cancer cytotoxicity 

Notes

Acknowledgements

This project was financially supported by King Saud University, Vice Deanship of Research Chairs, Kayyali Chair for Pharmaceutical industry, through the Grant No. MS-2018.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© American Association of Pharmaceutical Scientists 2019

Authors and Affiliations

  • Abdullah M. E. Youssof
    • 1
  • Fars K. Alanazi
    • 1
  • Mounir M. Salem-Bekhit
    • 1
    • 2
  • Faiyaz Shakeel
    • 1
  • Nazrul Haq
    • 1
    Email author
  1. 1.Kayyali Chair for Pharmaceutical Industry, Department of Pharmaceutics, College of PharmacyKing Saud UniversityRiyadhSaudi Arabia
  2. 2.Department of Microbiology and Immunology, Faculty of PharmacyAl-Azhar UniversityCairoEgypt

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