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AAPS PharmSciTech

, 20:51 | Cite as

Paclitaxel Encapsulation into Dual-Functionalized Multi-Walled Carbon Nanotubes

  • Vishakha Rathod
  • Rahul Tripathi
  • Parth Joshi
  • Prafulla K. Jha
  • Pratap Bahadur
  • Sanjay TiwariEmail author
Research Article

Abstract

This work reports the synthesis of multi-walled carbon nanotubes (CNTs) from xylene/ferrocene using catalytic chemical vapor deposition technique. Following characterization using transmission electron microscopy (TEM), energy-dispersive X-ray (EDX), and Raman spectroscopy, CNT surface was dual-functionalized using ethylenediamine and phenylboronic acid groups. Average diameter of CNTs was calculated to be 16.5 nm. EDX spectra confirmed the existence of carbonaceous deposits on the tube’s surface. Scattered electron diffraction and X-ray peak broadening calculations showed consistent inter-planer distance of the grown CNTs. Chemical functionalization, confirmed from FT-IR and Raman spectra, showed an enhanced dispersibility of CNTs in water. We describe the changes in the first- and second-order regions of the Raman spectra following the encapsulation of an anti-cancer drug, paclitaxel (PLX), into the free volume of functionalized CNTs. High PLX loading, achieved through its non-covalent π–π stacking within the CNT interior, is confirmed through the blue-shifted, softened G band in the Raman spectrum. While not addressed here, we will exploit this dual functionalization tactic to elaborate the relative role of attached moieties in the affinity interaction of CNTs with extra-cellular sialic acid, a biological target showing metastatic stage-dependent over-expression in colon cancer cells.

KEY WORDS

carbon nanotubes drug targeting aqueous dispersibility paclitaxel non-covalent interaction 

Abbreviations

CNTs

carbon nanotubes

CVD

chemical vapor deposition

PLX

paclitaxel

SA

sialic acid

TEM

transmission electron microscopy

EDX

energy-dispersive X-ray spectroscopy

SAED

ethylenediamine, selected-area electron diffraction

EDA

ethylenediamine

PBA

phenylboronic acid

Boc

di-tert-butyl dicarbonate

EDC

1-ethyl-3-(3-dimethylaminopropyl) carbodiimide

NHS

N-hydroxy succinimide

XRD

X-ray diffraction

HPLC

high-performance liquid chromatography

DLS

dynamic light scattering

RT

room temperature

Notes

Acknowledgments

The project was supported by grants received from the Science & Engineering Research Board (SERB), New Delhi (# ECR/2017/000903) and the B.U. Patel Research Promotion Scheme (# UTU-RPS/2017/88), India. The authors acknowledge the support of Professor N.P. Lalla (UGC-DAE CSR, Indore) for the TEM experiments.

Compliance with Ethical Standards

Conflict of Interest

Authors report no conflict of interest.

Supplementary material

12249_2018_1218_MOESM1_ESM.docx (1.4 mb)
ESM 1 (DOCX 1411 kb)

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Copyright information

© American Association of Pharmaceutical Scientists 2019

Authors and Affiliations

  1. 1.Maliba Pharmacy CollegeUka Tarsadia UniversitySuratIndia
  2. 2.Pharmaceutics DivisionPERD CentreAhmedabadIndia
  3. 3.Department of PhysicsUka Tarsadia UniversitySuratIndia
  4. 4.Department of Physics, Faculty of ScienceThe Maharaja Sayajirao University of BarodaVadodaraIndia
  5. 5.Department of ChemistryVeer Narmad South Gujarat UniversitySuratIndia

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