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AAPS PharmSciTech

, Volume 19, Issue 8, pp 3734–3741 | Cite as

Repositioning of Anti-parasitic Drugs in Cyclodextrin Inclusion Complexes for Treatment of Triple-Negative Breast Cancer

  • Josefina Priotti
  • María Virginia Baglioni
  • Agustina García
  • María José Rico
  • Darío Leonardi
  • María Celina Lamas
  • Mauricio Menacho Márquez
Research Article
  • 71 Downloads

Abstract

Drug repositioning refers to the identification of new therapeutic indications for drugs already approved. Albendazole and ricobendazole have been used as anti-parasitic drugs for many years; their therapeutic action is based on the inhibition of microtubule formation. Therefore, the study of their properties as antitumor compounds and the design of an appropriate formulation for cancer therapy is an interesting issue to investigate. The selected compounds are poorly soluble in water, and consequently, they have low and erratic bioavailability. In order to improve their biopharmaceutics properties, several formulations employing cyclodextrin inclusion complexes were developed. To carefully evaluate the in vitro and in vivo antitumor activity of these drugs and their complexes, several studies were performed on a breast cancer cell line (4T1) and BALB/c mice. In vitro studies showed that albendazole presented improved antitumor activity compared with ricobendazole. Furthermore, albendazole:citrate-β-cyclodextrin complex decreased significantly 4T1 cell growth both in in vitro and in vivo experiments. Thus, new formulations for anti-parasitic drugs could help to reposition them for new therapeutic indications, offering safer and more effective treatments by using a well-known drug.

KEY WORDS

albendazole repositioning breast cancer cell line cyclodextrin 

Notes

Acknowledgements

J.P. and M.V.B. are grateful to CONICET for Doctoral Fellowships. The authors also thank Ferromet S.A. (agent of Roquette in Argentina) for their donation of β-CD.

Funding Information

This study received financial support from the Universidad Nacional de Rosario and CONICET (Project N° PIP 112-201001-00194) and “Instituto Nacional del Cáncer.”

Compliance with Ethical Standards

All experiments were performed in accordance with the Canadian Council on Animal Care guidelines.

Animal Studies

All institutional and national guidelines for the care and use of laboratory animals were followed. This study was authorized by the Ethics Committee for Animal Use (registration number 1659/2016).

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Copyright information

© American Association of Pharmaceutical Scientists 2018

Authors and Affiliations

  • Josefina Priotti
    • 1
  • María Virginia Baglioni
    • 2
  • Agustina García
    • 3
  • María José Rico
    • 2
  • Darío Leonardi
    • 1
    • 3
  • María Celina Lamas
    • 1
    • 3
  • Mauricio Menacho Márquez
    • 2
    • 4
  1. 1.IQUIR-CONICETRosarioArgentina
  2. 2.Instituto de Genética Experimental, Facultad de Ciencias MédicasUNRRosarioArgentina
  3. 3.Departamento de Farmacia, Facultad de Ciencias Bioquímicas y FarmacéuticasUNRRosarioArgentina
  4. 4.Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario (IIDEFAR, UNR-CONICET)UNRRosarioArgentina

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