Table 2 Antifungal activity of cold and hot acetone extracts of neem, madhuca, and simarouba oil-seed cake

From: The antimicrobial and antioxidant property, GC–MS analysis of non-edible oil-seed cakes of neem, madhuca, and simarouba

Oil-seed cake/extraction solvents/Standard antibiotics ZI (mm) in test fungal isolates1
  Extract
Conc (%)
Fo2 Cc2 Af3 Ca3
Neem seed-cake
Cold-acetone 50 0 0 3.0 ± 0.1 0
  100 8.0 ± 0.1 0 6.0 ± 0.1 0
  200 13.0 ± 0.1 0 13.0 ± 0.1 0
Hot-acetone 50 6.0 ± 0.15 4.0 ± 0.1 0 0
  100 8.0 ± 0.05 9.2 ± 0.1 4.0 ± 0.05 0
  200 11.2 ± 0.1 14.16 ± 0.08 11.2 ± 0.1 0
Madhuca seed- cake
Cold-acetone 50 6.0 ± 0.1 0 0 0
  100 11.0 ± 0.1 5.0 ± 0.08 0 2.0 ± 0.1
  200 17.03 ± 0.1 11.2 ± 0.1 7.0 ± 0.1 5.0 ± 0.1
Hot-acetone 50 4.0 ± 0.1 0 0 0
  100 9.0 ± 0.05 5.0 ± 0.1 0 0
  200 12.1 ± 0.1 14.2 ± 0.1 7.0 ± 0.1 0
Simarouba seed-cake
Cold-acetone 50 0 7.0 ± 0.08 0 0
  100 7.0 ± 0.1 10.0 ± 0.08 0 0
  200 14.0 ± 0.1 15.0 ± 0.1 0 0
Hot-acetone 50 9.0 ± 0.1 0 2.0 ± 0.1 0
  100 11.0 ± 0.05 4.0 ± 0.1 4.0 ± 0.1 0
  200 14.0 ± 0.1 9.0 ± 0.1 6.0 ± 0.1 0
Standards
Fluconazole 54 6.2 ± 0.1 5.2 ± 0.1 5.6 ± 0.1 6.6 ± 0.1
Bavistin 5 12.0 ± 0.1 11.0 ± 0.1 12.0 ± 0.1 0
DMSO 205 0 0 0 0
  1. 1Values are mean inhibition zone of three replicates (n = 3); crude extracts were dissolved in DMSO (200 mg 1 ml of DMSO). Each well received 20 µl (400 µg ml−1)
  2. 2Plant pathogenic fungal isolates: Fo – Fusarium oxysporum and Cc – Colletotrichum capsici
  3. 3Human pathogenic fungal isolates: Af- Aspergillus flavus and Ca – Candida albican
  4. 45 mg of standards dissolved in 1 ml of DMSO 520 µL of DMSO (negative control) added to each well