Abstract
Purpose
The aim of this study is to test the hypothesis that feeding trans fatty acids (TFA) (5%) along with fructose exacerbates obesity and non-alcoholic fatty liver disease (NAFLD) in rats.
Methods
Male Wistar rats were randomized into four groups, i.e., standard diet, 5% TFA + standard diet, fructose + standard diet, and TFA + fructose + standard diet. All the diets were provided for 16 weeks. The body weight, body mass index, calorie intake, adiposity index, and liver index were determined. Serum glucose, insulin, lipid profile, and liver enzymes were estimated. Liver lipids, markers of oxidative stress, inflammation, and collagen were estimated in the liver. The histopathological evaluation of the adipose tissue and liver were carried out.
Results
TFA + standard diet caused an increase in body weight while TFA + fructose + standard diet caused significant body weight gain, adiposity index, and hypertrophy of adipocytes. TFA + fructose + standard diet caused insulin resistance and dyslipidemia in the rats. Rats in the TFA + standard diet group showed marked hepatic steatosis and an elevation in alanine aminotransferase, while those in the TFA + fructose + standard diet group showed oxidative stress, inflammation, and fibrosis in the liver.
Conclusion
Feeding of TFA at a concentration of 5% along with the standard diet resulted in an increase in the body weight and hepatic steatosis, but the addition of fructose to 5% TFA and standard diet resulted in obesity and non-alcoholic steatohepatitis. Thus, the reduction in TFA content of foods must be accompanied by a significant decrease in the fructose intake in order to protect against obesity and NAFLD.
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Abbreviations
- AC/TC:
-
ratio-abdominal circumference to thoracic circumference ratio
- ALP:
-
alkaline phosphatase
- ALT:
-
alanine aminotransferase
- AST:
-
aspartate aminotransferase
- BMI:
-
body mass index
- GGT:
-
gamma-glutamyl transferase
- GSH:
-
reduced glutathione
- HDL-C:
-
high density lipoprotein cholesterol
- HOMA-IR:
-
homeostasis model assessment-insulin resistance
- LDL-C:
-
low density lipoprotein cholesterol
- MDA:
-
malondialdehyde
- NAFLD:
-
non-alcoholic fatty liver disease
- NASH:
-
nonalcoholic steatohepatitis
- PHVO:
-
partially hydrogenated vegetable oil
- SOD:
-
superoxide dismutase
- TFA:
-
trans fatty acids
- TG:
-
triglycerides
- VLDL-C:
-
very low density lipoprotein cholesterol
- WHO:
-
World Health Organization
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Acknowledgements
The authors are grateful to Ms. Deepali Ganachari and Ms. Jaya Verma, research fellows at Dept. of Clinical Pharmacology, T.N. Medical College and BYL Nair Hospital for their help during animal experimentation, and Dr. Sanjay Pawar, veterinary pathologist for carrying out the histopathological evaluation of the liver and adipose tissue.
Funding
The University Grants Commission (BSR Fellowship), New Delhi, India, letter no. F.25–1/2014–15 (BSR)/No. F.5–63/2007 (BSR) dated 16 Feb 2015 is awarded to Ms. Sarayu Pai.
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Contributions
SAP carried out the experiments and wrote the first draft of the manuscript. RPM helped in designing the study and analyzing the data. ARJ was involved in designing the study and supervision of the conduct of the work. All the authors read and approved the final manuscript.
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Ethics approval
The protocol was approved by the Institutional Animal Ethics Committee of T N Medical College and BYL Nair Charitable Hospital, Mumbai, India (protocol no. IAEC/2016/8 dated 29 February 2016).
Conflict of interest
The authors declare that they have no conflict of interests.
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Pai, S.A., Munshi, R.P. & Juvekar, A.R. Partially hydrogenated vegetable oil containing 5% trans fats when combined with fructose exacerbates obesity and non-alcoholic fatty liver disease in rats. Nutrire 45, 5 (2020). https://doi.org/10.1186/s41110-019-0105-6
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DOI: https://doi.org/10.1186/s41110-019-0105-6