Postoperative infection caused by Acinetobacter baumannii misdiagnosed as a free-living amoeba species in a humeral head hemiarthroplasty patient: a case report
Acinetobacter baumannii is ubiquitous, facultative intracellular, and opportunistic bacterial pathogen. Its unique abilities allow it to survive in a diverse range of environments, including health care settings, leading to nosocomial infections. And its exceptional ability to develop resistance to multiple antibiotics leaves few drug options for treatment. It has been recognized as a leading cause of nosocomial pneumonia and bacteremia over the world.
In this case, a 73-year-old woman presented with a Neer Group VI proximal humeral fracture. Six hours after a successfully performed hemiarthroplasty, she developed continuous fever. Clinical examination revealed that the vitals were regular. Laboratory and radiographic examinations revealed only elevated procalcitonin levels. Blood culture revealed no bacterial or fungal growth. Cooling treatment and empirical broad-spectrum antibiotic therapy showed no apparent effect.
We report a postoperative infection caused by Acinetobacter baumannii. The infectious pathogen was identified via molecular DNA sequencing and was initially misidentified as a free-living amoeba species upon microscopic examinations. The patient was mistreated with antiamebic combination therapy. Her symptoms persisted for over 4 months and were eventually followed by her death.
KeywordsAcinetobacter baumannii Free-living amoebae Hemiarthroplasty Case report
Compounded sulfamethoxazole and sulfadiazine
Fever of unknown origin
Intensive care unit
Minimum inhibitory concentration
Polymerase chain reaction
Please see Additional file 1 for translations of the abstract into the five official working laguages of the United Nations.
During the past few decades, Acinetobacter baumannii has received significant attention from scientific and medical communities [1, 2, 3, 4, 5]. Its unique abilities, such as intrinsically resistance to desiccation, allow it to survive in a diverse range of environments, including health care settings, leading to nosocomial infections and troublesome outbreaks . As a nosocomial pathogen which is most commonly involved in hospital infections, A. baumannii has an exceptional ability to develop resistance to multiple antibiotics, leaving few drug options for treatment [3, 6, 7, 8]. Inappropriate initial antimicrobials were strongly associated with increased mortality for A. baumannii infections . In various parts over the globe, it has been recognized as a leading cause of nosocomial pneumonia and bacteremia [10, 11, 12, 13].
Clinically, A. baumannii usually affects fragile, immunocompromised patients, especially those who suffer from burns, have trauma, or are in the intensive care units (ICUs) . Those infected are commonly associated with mechanical ventilation, intravenous and urinary catheterization, surgery, invasive procedures, and prolonged broad-spectrum antimicrobials [15, 16, 17, 18, 19, 20]. Clinical reports suggest that Acinetobacter can cause serious, life-threatening infections .
In this case report, we describe a rare case of infection caused by Acinetobacter baumannii misdiagnosed as a free-living amoeba (FLA) species and mistreated with an antiamebic combination therapy with metronidazole, fluconazole, compounded sulfamethoxazole and sulfadiazine (COSMZ), dihydroartemisinin and piperaquine phosphate combined (Duo-Cotecxin), and meropenem in a 73-year-old woman admitted to the Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
In this case, the patient stayed in hospital for 134 days. On the basis of the maximum body temperature and laboratory examinations, hospitalization was divided into three stages, namely, fever of unknown origin (FUO) stage (days 1–36), antiamebic treatment (AT) stage (days 37–85), and treatment failure (TF) stage (days 86–134) (Additional files 2 and 3).
Fever of unknown origin stage (FUO, days 1–36)
Antiamebic treatment stage (AT, days 37–85)
On day 37, after the diagnosis of FLA infection was made, the patient was immediately started on a combination therapy with metronidazole, fluconazole, compounded sulfamethoxazole and sulfadiazine (COSMZ), and meropenem [22, 23, 24, 25, 26]. Meropenem was used in case that the patient was infected with other pathogens in addition to FLA. On day 52, Duo-Cotecxin (Beijing Holley-Cotec Pharmaceuticals Co., Ltd., China) was added to the antiamebic therapy. Additionally, the medical team attempted to obtain miltefosine, which did not arrive in time. From day 61–85, remission of clinical manifestations of the patient was observed. During this period, her mental status remained stable, no schizophrenia related symptoms appeared, and the body temperature was lower than 38.0 °C most of the time (Fig. 2b). Laboratory results were normal. A decrease in FLA-like pathogen from 5.25 to 1.95 counts under high power field at magnification (× 100) field in blood samples was also observed by microscopy (Fig. 3b).
Treatment failure stage (TF, days 86–134)
Although a remission of clinical manifestations of the patient was observed for approximately 25 days (days 61 to 85), the patient’s condition deteriorated from day 86. Her body temperature was higher than 38.0 °C most of the time (Fig. 2b), and the FLA-like pathogen count in the blood samples as determined by microscopy increased again to 3.85 (Fig. 3b). Although frequently received blood transfusions (10 times comprised of 1 international unit of red cell suspension liquid and 100 ml of blood plasma, or 2 international unit of red cell suspension liquid and 200 ml of blood plasma each time), the patient became severely anemic. Her hospital course was complicated by refractory hypernatremia and hyperchloremia (sodium–153 mmol/L, chloride–114 mmol/L), leading to multiple blisters on the left leg (Fig. 1d). FLA-like pathogens were also detected in the blister fluid samples. She finally died of multiple organ failure and intractable fever on the 134th day of hospitalization.
Prior to observing in the microscope, blood, CSF and blood samples were processed with the physiological saline method of direct smears.
In the blood samples, many cells looked like “straw hats” (Fig. 3a). The cells were discoid in shape; the cytoplasmic humps were surrounded with flattened hyaline margins, some of which were fan-shaped. The surfaces of the cells were warty. The dimensions of these cells ranged from 10.2–25.4 × 11.8–26.5 μm with average dimensions of 17.8 × 19.3 μm. Smaller cells were easily observed. Owing to the granular mass on the surface, the nucleus could be not clearly seen. Locomotion was not visible in most microscopic fields. But sometimes cells displayed short blunt or fila-pseudopodia and slow deformation (Additional file 4).
Additional file 4: Video. Short video clip of FLA-like pathogen in blood sample under microscopic vision. Locomotion was not visible in most microscopic fields. But sometimes cells displayed short blunt or fila-pseudopodia and slow deformation. (MP4 18 599 kb)
In CSF samples, we found different cells that appeared irregular and had vacuoles (Fig. 4a-c) and finger pseudopodia (Fig. 4c). Some had circular shells that looked transparent, but no structures could be observed (Fig. 4d). Occasionally, black spheres were observed (Fig. 4e).
In urine samples, single cells displayed different morphological forms; some were spherical (Fig. 4f-h, j, l, n), whereas others had huge vacuoles (Fig. 4k) and/or possessed fan-shaped pseudopodia (Fig. 4m). Some “cells” clustered together.
After the antiamebic treatment, when the body temperature of the patient came down, the discoid-like unicellular body could not be detected in blood samples (Fig. 3c g-n). Most forms were oval; the cell bodies were approximately 13.0–16.1 μm long and 8.8–11.7 μm wide with average dimensions of 15.0 × 10.1 μm. Locomotive cells displayed short filopodia or conical non-branching pseudopodia arising from apical and/or lateral parts of the cell (Fig. 3c k-n). Furthermore, some small cells showing small, refractive outlines with single long (over 10 μm) filopodia (Fig. 3c g, h) and large irregularly shaped cells (more than 10 μm) with short (3–5 μm) filopodia (Fig. 3c i, j) could be seen occasionally.
The morphological characteristics of the pathogen resemble species of FLA, Vannella sp. , and another type of scale-bearing amoeba: Cochliopodium sp. , and with no similarity with known pathogenic amoeba such as Acanthamoeba sp., Balamuthia mandrillaris, Entamoeba histolytica, Naegleria fowleri, Sappinia diploidea, etc. .
To confirm the morphological findings and further discover the cause of the infection, uncultured blood serum samples were sent for molecular identification. The blood serum samples were taken on day 44 and preserved in liquid nitrogen. Total genomic DNA was extracted from the samples and polymerase chain reaction (PCR) was performed using prokaryotic universal primers and eukaryotic universal primers (Additional file 5). Primer sequences were 16 s-27F 5′-AGAGTTTGATCATGGCTCAG-3′, 16 s-1390R 5′-ACGGGCGGTGTCTACAA-3′, 18S-F 5′-ACCTGGTTGATCCTGCCAGT-3′, 18S-R 5′-CTTGTTACGACTTTTACTTCC-3′, 18S-1080F 5′-GGGRAACTTACCAGGTCC-3′ and 18S-1578R 5′-GTGATRWGRTTTACTTRT-3′. The obtained sequencing data (1300 bp) using prokaryotic primers were compared with all published sequences in GenBank using BLASTn at National Center for Biotechnology Information (http://blast.ncbi.nlm.nih.gov/) and submitted to the GenBank database (Accession No. MG581460). Results showed that the sequence had a maximum of 80% identities with multiple Acinetobacter baumannii strains (Additional file 6). Comparison of the obtained sequence with Acinetobacter baumannii strain KAB05, complete genome (Accession No. CP017650.1) was shown in Additional file 7. Another sequencing data (1800 bp) were obtained using eukaryotic universal primers pair 18S-F/18S-R and had a maximum of 99% identities with Human Genomic sequences. No sequencing data were obtained using universal primers pair 18S-1080F/18S-1578R.
We report a case of postoperative infection in a humeral head hemiarthroplasty patient caused by Acinetobacter baumannii, which was identified via molecular DNA sequencing. The pathogen was initially misidentified as a FLA species.
A. baumannii is recognized as an opportunist pathogen which causes infections in fragile patients [30, 31]. Infections caused by A. baumannii are usually associated with defects in anatomical host defenses and alteration of normal host flora by exposure to broad-spectrum antibiotics , affecting mainly severely ill patients in the ICUs, and patients who have trauma or suffer from burns [3, 32]. The most common mode of transmission is via the hands of health care workers [33, 34]. The most common clinical manifestations of A. baumannii are nosocomial pneumonia and bacteremia [16, 35, 36, 37, 38, 39, 40].
The exact time point of infection could not be established by now, but was speculated to be after the surgery. The route of infection in our patient is not clear. After scrutinizing the whole hospital course, we summarize the reasons for misdiagnosis and mistreatment into three points. First, clinical manifestations of this patient after infection were atypical. Common clinical manifestations of A. baumannii include nosocomial pneumonia, bacteremia, wound infections and osteomyelitis, urinary tract infections, endocarditis, and meningitis [16, 35, 36, 37, 38, 39, 40]. The patient only developed continuous fever. The operated upper limb healed uneventfully, and none of the common manifestations of A. baumannii above were developed. Second, blood culture was performed several times and revealed no bacterial growth. Two antibiotic agents used in FUO stage, namely, meropenem and cefoperazone/sulbactam, had antimicrobial activity against A. baumannii  and were among current treatment options to A. baumannii infections [32, 42, 43]. Negative results of blood culture may due to the use of meropenem and cefoperazone/sulbactam, along with other antimicrobial treatments. The last and most important, the pathogens observed microscopically were FLA-like, rather than A. baumannii. Its microscopic characteristics resemble Vannella sp. , and another type of scale-bearing amoeba: Cochliopodium sp. . Diagnosis of FLA infection was mainly based on the microscopic findings. In addition, after the initiation of antiamebic therapy, a clinical remission was observed, making us further believe that the causative pathogen was a FLA species.
The sequences obtained using prokaryotic universal primers were compared with all published sequences in GenBank. According to the results from molecular DNA sequencing, the final diagnosis is A. baumannii infection. However, this alone cannot explain the discovery of many FLA-like pathogens microscopically.
Two hypotheses were established for this, though neither can be verified by now. One hypothesis is that the patient was infected with two pathogens, FLA and A. baumannii. The FLA could be a Vannella species based on its morphology and may serve as host for A. baumannii. Some of the Vannella species are known to harbor bacterial pathogens in previous studies [44, 45]. The empirical broad-spectrum antibiotic therapy destroyed most of the FLA later, leaving A. baumannii in the predominant position. Another hypothesis is the strange FLA-like pathogens were actually the blood cells or parts of them, which were invaded by A. baumannii and serve as hosts for them. Bacteria living within eukaryotic cells are called intracellular bacteria. Obligate intracellular bacteria enter into cells and use host cell resources for their replication , while facultative intracellular bacteria can multiply both inside and outside host cells . Intracellular bacteria generally reside directly in the host cytoplasm or in host-derived vacuoles , some can even invade eukaryotic nucleus . They frequently hijack the host endocytic and secretory pathway, and structure the host a specialized cell for their replication . Many intracellular bacteria are well-known pathogens, including Legionella pneumophila , Francissella tularensis , and Mycobacterium tuberculosis . A. baumannii is also facultative intracellular bacteria , and had been reported to adhere and invade into human pulmonary cells, causing respiratory infections and pneumonia [55, 56, 57].
A. baumannii infections are commonly difficult to treat as the causative strains often have broad antimicrobial resistance. These A. baumannii strains are classified as multidrug-resistant (MDR), extensively drug-resistant (XDR) and pandrug-resistant (PDR) based on their antimicrobial susceptibility profile. MDR strains are resistant to three or more, XDR strains are resistant to all but one or two, and PDR are resistant to all classes of potentially effective antimicrobial agents .
Current treatment options to A. baumannii infections include carbapenems, sulbactam, tigecycline, fluoroquinolones, aminoglycosides, colistin and rifampin [32, 42, 43]. Carbapenems have been regarded as the treatment of choice for severe A. baumannii infections [59, 60]. Strains respond briskly to carbapenems if they retain susceptibility to this antimicrobial class [15, 32]. However, increasing carbapenem-resistant A. baumannii strains are reported worldwide. Sulbactam is the most active of the β-lactamase inhibitors and has intrinsic antimicrobial activity against A. baumannii . Sulbactam has shown promising results against A. baumannii strains with various susceptibility profiles [61, 62, 63, 64], but its antimicrobial activity has declined substantially recently [65, 66]. Another option to treat A. baumannii infections is tigecycline. Tigecycline is a broad-spectrum antibiotic with bacteriostatic activity against A. baumannii [67, 68] and is commonly reserved for salvage therapy . If β-lactams cannot be used, fluoroquinolones and aminoglycosides can be considered as potential treatment options . For XDR A. baumannii, polymyxins are often the last treatment option. Unfortunately, polymyxins suffer from high rates of nephrotoxicity and neurotoxicity and possess no therapeutic window . Recent in vitro models suggest that rifampicin maintains a high level of activity against A. baumannii, even in carbapenem-resistant strains [42, 70]. However, rifampicin should not be used in monotherapy as it induces the resistance of A. baumannii to itself .
In the present case, although meropenem and cefoperazone/sulbactam, which were among treatment options to A. baumannii infections [32, 42, 43], were used in this case. However, these antibiotics were not used according to the treatment guidelines for A. baumannii and may not reach the minimum inhibitory concentration (MIC). In addition, the A. baumannii strains in this case report may be carbapenem-resistant, MDR, or even XDR, often leading to very high mortality.
This report presents a case of A. baumannii identified by molecular DNA sequencing, which was initially misidentified as a FLA species. Medical workers should be aware that patients associated with mechanical ventilation, surgery and invasive procedures, and prolonged broad-spectrum antimicrobials, especially those who suffer from burns, have trauma, or are in the ICUs, are among high risk-population of A. baumannii infection. If these patients develop FUO, A. baumannii infection should be taken into consideration. Clinically, due to the use of empirical antibiotics, blood culture results may be negative, and pathogen morphology under microscopic examination is sometimes atypical. In that case, the causative pathogen should be identified by molecular DNA sequencing if possible.
The authors are grateful to the patient’s relative for full consent to the publication of this case report, and would like to thank the National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, for providing Duo-Cotecxin in the treatment of the patient.
This work was supported by grants from National Natural Science Foundation of China (31271015), and Key Basic Research Project of Science and Technology of Shanghai (13JC1403900).
Availability of data and materials
The datasets generated and/or analyzed during the current study are not publicly available since the medical records and data are the patient’s privacy, but are available from the corresponding author on reasonable request under the consent from close relatives of the patient.
Jiaxin Tang performed the data, made the picture and wrote the manuscript. HZ and LC carried out the parasite detection and species identification. ZQ supervised the development of the manuscript. CY, ZQ, TT, Jian Tang, YS, ML and KD participated in the patient’s treatment. All authors read and approved the final manuscript.
Ethics approval and consent to participate
Consent for publication
Written informed consent was obtained from the relative of the patient for publication of this Case Study and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
The authors declare that they have no competing interests.
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