Extracellular vesicles derived from different sources of mesenchymal stem cells: therapeutic effects and translational potential

Abstract

Mesenchymal stem cells (MSCs) were known to have excellent properties in cell therapy. However, the risk of immune rejection associated with cell transplant therapy hampers its use. Extracellular vesicles secreted by MSCs derived from different sources that contain therapeutic molecules such as RNA and proteins, which is a novel strategy for cell-free therapy. Recently, researches show EVs from MSCs (MSC-EVs) of different sources have special functions and effects on different diseases. Here, we collected these researches and compared them to each other. In addition, their potential and possible application in clinical treatment are described.

Introduction

Mesenchymal stem cells(MSCs)are kinds of stem cells that play an important role in regeneration and restoration because of its multi-lineage differentiative capacity. They have the potential to differentiate to form connective tissues, skeletal muscle cells, and cells of the vascular system [1]. MSCs are developed from the mesodermal germ layer, which is one of the three layers in the inner mass cell [2]. There are a large number of MSCs in the parts of human bodies with high regeneration and differentiation capacity,such as bone marrow, embryo. Besides, the human umbilical cord and adipose tissue are also important origins of MSC for researches. The above four sources are the main sources of MSC researches and are also the main kinds we are going to introduce in this review. In addition to this, there are some rare sources of MSCs, including menstrual blood-derived MSCs [3, 4] and dental MSCs [5]. What they secreted have characteristics of MSCs and the potential for treatments of related diseases. Moreover, it has been discovered that MSCs also played an important role in immune regulation and might be a mediator of inflammation. The signals of inflammation activate MSCs to differentiate proinflammatory and anti-inflammatory phenotype and MSCs can also affect the polarization of monocytes and control pathogenic T cell responses [6, 7]. More recently, it was reported that MSCs transplanted into human bodies for treatments and received good effects. Thus, A new treatment called cell-therapy has emerged and developed [8]. Although MSCs transplantation seems to have a promising prospect for development, most clinical trials remained in phase I or II. And clinical trial failures of autologous and allogeneic MSC products have been frequent. There are even reports about the risk of tumorigenicity and cell death resulted from the transplantation of MSCs [9].

It has been shown that the therapeutic functions of MSCs mediated partly through paracrine effects. The various bioactive molecules that MSCs secreted can modulate immune, inhibit apoptosis and fibrosis, promote angiogenesis and the growth of stem and progenitor cells [10]. Some of them are mediated by extracellular vesicles (EVs), which were a series of vesicles secreted by MSCs (Fig. 1). EVs are cell-derived membranous structures that originate from the endosomal system or which are shed from the plasma membrane [11]. EVs are not a homogeneous system, including exosomes, shedding vesicles, apoptotic bodies, melanosomes, and prostasomes range from 10 to 1000 nm [12, 13]. Most researches mainly focused on exosomes and microvesicles (MVs). MVs are vesicles of which the particle size is > 200 nm, budding directly from the plasma membrane [14]. Exosomes have the smaller particle size that ranges from 50 nm to 200 nm, for they are formed through the invagination of the early endosome [15]. Unlike microvesicles, exosomes are cup-like vesicles with CD81, CD9, and Alix as their biomarkers, due to the different mechanisms of secretion [1, 16]. Exosomes also have some special biological characteristics and processes. Apart from those proteins that serve as biomarkers, exosomes also carry many functional cytokines and growth factors, regulatory RNAs and so on [15]. The release of exosomes is also a special process. It involves some proteins, such as the ESCRT [17]. The function of exosomes that attract much attention. In the beginning, exosomes were regarded as metabolic waste [18]. With more and more studies of exosomes, the functions of signal transmission and cell-to-cell communication were discovered [19]. On the one hand, they appear similar functions of MSCs and is expected to become the alternatives to cell therapies. On the other hand, it has been discovered that exosomes might influence the tumor microenvironment, but the mechanism remained unknown [20]. Thus, exosomes have the potential to treat cancers by changing the microenvironment of tumors.

Fig. 1
figure1

MSCs derived from different sources and EVs secreted. MSCs are mainly extracted from bone marrow, human umbilical cord, embryonic cells, and adipose tissue. And they secrete EVs such as microvesicles and exosomes

In addition, EVs from different MSCs may show a unique tendency to the therapy of some diseases. And they appeared differently in the same diseases [4, 21]. There are several investigations showed the potential therapeutic effects in diseases of cardiac injury, kidney injury, and brain injury [12]. Recently, MSC-derived EVs as a cell-free therapeutic alternative have raised considerable interests. Compared to MSCs, they reduced the risk of cell transplantation and amplification. At the same time, they have the advantages of good stability. Not only that, but EVs can also be used as a vehicle to deliver bioactive factors. Moreover, EVs were proved to have the ability to cross the blood–brain barrier [22]. Considered these advantageous properties of the MSC-EVs, further studies need to be done.

In this review, we mainly compared the therapeutic effects of four different extracellular vesicles of mesenchymal stem cells (MSCs-EVs) and discussed their potential applications in diseases which include that they act as carriers of active biological molecules.

EVs from bone marrow mesenchymal stem cells (BM-MSCs)

The therapeutic effects of EVs from BM-MSCs

Extracellular vesicles derived from bone marrow mesenchymal stem cells is the most common one. And it is also a conventional way to obtain the MSCs-EVs, which is to harvest bone marrow from adult rats or humans [23, 24]. For extracellular vesicles from human bone marrow mesenchymal stem cells (hBM-MSCs), the most common diseases treated are cartilage defects or osteoarthritis which are related to bone diseases [25] [26]. The reason is that the bone marrow source has the bone targeting ability [27] and can also induce osteogenic differentiation similar to its parent cells [28, 29]. In a study of osteoarthritis, EVs from hBM-MSCs can promote extracorporeal cartilage regeneration because EVs stimulated chondrocytes to produce proteoglycans and type II collagen. Also, those EVs inhibit the adverse effects of inflammatory mediators on cartilage homeostasis. These anti-inflammatory effects were mediated by inhibiting TNF-alpha-mediated upregulation of COX2 and pro-inflammatory interleukins [26]. Since the bone marrow from which hMSC comes is connective tissue, the EVs they secreted were discovered to have a therapeutic effect on other connective tissue such as tendon. Topical administration of MSC-EVs promotes tendon healing by inhibiting the accumulation of inflammatory and apoptotic cells and increasing the proportion of tendon resident stem cells/progenitor cells [30].

EVs from hBM-MSCs have also shown good efficacy in the treatment of the renal injury. For chronic kidney injury caused by long-term exposure to heavy metals such as cadmium, Intravenous injection of high-purity EVs from hBM-MSCs repaired damage to renal proximal tubules, glomerular podocytes, bone deformation, and improved survival [31]. And in acute kidney injuries, EVs have a similar effect to parent cells in promoting kidney regeneration [32]. For diabetic nephropathy, EVs inhibit renal fibrosis and result in the downregulation of several fibrogenic genes in renal tissue [33].

In the aspect of anti-inflammation, EVs from hBM-MSCs works in several different ways. First, they downregulated the production of IL-23 and IL-22 and enhanced the anti-inflammatory phenotype of mature human regulatory macrophages (Mregs) [34]. Second, as mentioned in osteoarthritis treatment, they inhibited TNF-alpha-induced expression of COX2 and expression of pro-inflammatory interleukins [26].

Another application of EVs from hBM-MSCs is Graft-Versus-Host Disease Amelioration. Systemic infusion of hBM-MSCs EVs could prolong the survival time of aGVHD mice and reduce pathological damage of various GVHD-targeted organs. This was due to the immunosuppressive effect of hBM-MSCs derived extracellular vesicles. CD4 + and CD8 + T cells were inhibited in EV-treated GVHD mice. While inhibiting the functional differentiation of T cells from naive to effector type, EVs derived from BM-MSCs also retained the CD4 + CD25 + Foxp3 + regulatory T cell (Tregs) population [35]. EVs can significantly reduce the risk of cell transplantation while retaining the similar efficacy of BM-MSCs. In another study about the immunoregulatory effects of hBM-MSCs EVs, they induced the apoptosis of CD3 + cells and of the CD4 + subpopulation and increased the proliferation and the apoptosis of Tregs. In addition, EVs treatment increased the Treg/Teff ratio and the concentration of the immunosuppressive cytokine IL-10. These results suggest EVs have an immunomodulatory effect on T cells in vitro, but this is not related to IDO, which is an established mediator of MSC immunosuppressive effects [36].

Compared to EVs from hMSC, mouse bone marrow derived MSC-EVs have similar functions in tissue regeneration [37], protective effects to neuron cells [38] and irradiated hematopoietic stem cells [39]. However, it has been discovered that EVs from human MSCs (hMSCs) are generally more effective than those from mouse MSCs (mMSCs) in a study of allergic airway inflammation in the mouse model. Moreover, blocking the release of EVs completely abrogated the effects of hMSCs but only partially inhibited the effects of mMSCs, suggesting different potential mechanisms of mMSC versus hMSC actions [40].

The therapeutic effects of exosomes from BM-MSCs

Exosomes are one type of extracellular vesicles, which size is smaller than others, for about 100 nm [17]. They have similar therapeutic effects to BM-MSCs and are expected to develop into a promising cell-free therapeutic strategy. In some studies, researchers investigated the potential mechanisms of human bone marrow mesenchymal stem cell derived exosomes (hBM-MSCs-Ex) in the treatment of liver fibrosis and found the therapeutic effect of exosomes on hepatic fibrosis was significantly greater than that of hBM-MSCs [41]. Moreover, recent studies have discovered that exosomes are the main mediators of MSC therapy in Autism Spectrum Disorder (ASD). In the previous study, transplanting human bone marrow mesenchymal stem cells (MSC) into the lateral ventricle of BTBR mice can improve their autistic phenotype for a long time. However, the nasal administration of MSC exosomes to BTBR mice can significantly improve autistic behavior that increased social interaction and reduced repetitive behaviors. This treatment can avoid the risk of rejection and infection associated with the transplant [42]. The exosomes, which are small size vesicles with strong permeability, are also very suitable for nasal administration. In addition, the role of hBM-MSCs-Ex in immunotherapy should not be ignored. Apart from inducing polarization of M2 macrophages, it also regulated the maturation, proliferation, and activation of lymphocytes. For example, the exosomes regulated the proliferation of T and B lymphocytes and affected the function of B lymphocytes by regulating the differential expression of related mRNAs [43].

More effects were investigated in mouse bone marrow MSCs derived exosomes. For inducing macrophage polarization, exosomes derived from mBM-MSCs have the same effect. In a study of wound healing, hBM-MSCs exosomes mainly regulated the polarization of macrophages by targeting pknox1 with mir-223 in exosomes [44]. However,in another study of heart attacks, LPS-primed mouse BMSC-derived exosomes had a good therapeutic effect on polarized M2 macrophages in vitro and reduce inflammation and myocardial cell apoptosis after infarction [45]. Similar to macrophage polarization, recent studies found that mouse BMSC-derived exosomes can regulate microglia polarization. The exosomes reduced inflammation and demyelination of the central nervous system in EAE rat models by increasing microglial cell M2 polarization [46]. In some studies of myocardial infarction, BMSC-derived exosomes showed therapeutic potential in improving myocardial function. GATA-4 is a cardiomyocyte-specific zinc finger transcription factor that regulates differentiation, growth, and survival. GATA-4-expressing BMSCs improve functional recovery than that observed for BMSCs alone when injected into the myocardium immediately after induction of ischemia. It was found that GATA-4-expressing mBM-MSCs exosomes can induce MSCs to differentiate into precursor cells of cardiomyocytes, reduce apoptosis of cardiomyocytes, and improve cardiac function after myocardial infarction [47]. Additionally, they significantly reduced the apoptosis rate of cardiac stem cells (CSCs) and the production of reactive oxygen species (ROS) after oxidative stress injury. And exosomes of hypoxic-cultured BM-MSCs were more potent than exosomes of normal cultured MSCs. Further researches showed that mir-214 is the main effector molecule in exosomes of BM-MSCs that protects CSCs from oxidative damage. BMSCs inhibited oxidative stress damage in CSCs by silencing the release of mir-214-containing exosomes by CaMKII [48]. Another study explains this phenomenon from another angle. Hypoxia culture enhanced the activity of mir-210 and nSMase2 in MSC and its secreted exosomes, which are important to the secretory pathway of exosomes [49].

The transferring function of EVs from BM-MSCs

As vesicles secreted by cells, the contents of EVs have something in common with BM-MSCs, such as RNA. Some specific microRNAs secreted by BM-MSCs regulate cells function through EVs transportation. For example, EVs derived from BM-MSCs contains miR-146a which is a famous anti-inflammatory microRNA. Studies about Inflammatory bowel disease (IBD) showed that overexpressing miR-146a inhibited TNF receptor-associated factor 6 (TRAF6) and IL-1 receptor-associated kinase 1 (IRAK1) expression in TNBS-induced colitis of rats. And the administration of EVs containing miR-146a down-regulated the increased phosphorylation levels of NF-κB p65 and IκBα, so that suppressed inflammation factors such as tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6) and Interleukin-1β [50].

For exosomes, a type of smaller and more homogeneous extracellular vesicles, studies on microRNAs associated with them have been more extensive. At present, many microRNAs contained in exosomes have been revealed to associate with organ repair and other functions. It has been reported that low levels of miR-224-3p in BM-MSCs derived exosomes promote endothelial cell proliferation, migration, invasion, and angiogenesis by targeting focal adhesion kinase family interacting proteins (FIP200) [51]. In a study of renal ischemia/reperfusion injury, researchers found that miR-199a-3p enriched in hBMSC-Exos and down-regulate Sema3A expression, thereby activating the AKT and ERK pathways when delivering to renal cells by exosomes [52]. Mir -199a-5p was another microRNA that has been showed to protect renal ischemia/reperfusion injury. It is transferred to renal tubular epithelial cells (nrk-52e) in a time-dependent manner and significantly inhibits I/r-induced er stress by targeting immunoglobulin (BIP) [53]. Besides, other microRNAs that BMSCs-Exo contained related to other functions have also been studied. For example, the release of exosomes from BM-MSCs in elderly mice can be absorbed by fat cells, muscle cells and liver cells, resulting in insulin resistance in vivo and in vitro, which was related to miR-29b-3p. Down-regulation of it significantly improved insulin resistance in older mice [54].

In addition to the transport of endogenous RNA, BM-MSCs EVs can also act as carriers for the transport of other small molecules. Because of its own therapeutic effect on some diseases or targeting ability or greater affinity to specific cells, it may be suitable for EVs to transport therapeutic components. As one type of EVs, microvesicles were found to be endogenous carriers for delivering paclitaxel to pancreatic cancer cells. Microvesicles capsuled Paclitaxel proved to have strong anti-proliferation activity on pancreatic cancer cells [55]. However, as for exosomes from BM-MSCs, they also serve as good carriers. Due to the ability to transfer active molecules (cytokines, growth factors, RNA), it was reported that they were used for the delivery of doxorubicin to breast cancer cells. Researchers demonstrated that with the chimeric LAMP2b-DARP in protein on the surface of exosomes, DOX-exo showed more efficient binding to HER2-positive TUBO cells. The designed exosomes increased the accumulation of DOX in the tumor site and reduce the growth rate of breast cancer cells [56].

Furthermore, some researchers have combined exosomes with other drug delivery systems so that improve the therapeutic effect of EVs. Small extracellular vesicles (sEVs) derived from BM-MSC are widely used in the treatment of myocardial infarction (MI). Researchers designed an alginate brine gel with sEVs (sEVs-gel) in it to increase its retention in the heart in order to improve treatment. Experiments proved that it reduced the apoptosis of cardiomyocytes and promoted the polarization of macrophages. Moreover, the sEVs-gel group had significantly better measurements of cardiac function and infarct area than the sEVs group [57].

To sum up, EVs from BM-MSCs have a wide application in many diseases and have been studied extensively. They showed the potential to be modified or engineered to suit clinical proposes. And they can be also administered in a variety of ways. However, the mechanisms by which it works needs further research.

EVs from human umbilical cord mesenchymal stem cells (huc-MSCs)

The therapeutic effects of EVs from huc-MSCs

The human umbilical cord is an excellent source of MSCs, as the human umbilical cord can be largely expanded and has clinical potential in the treatment of some diseases including nerve, cardiovascular, liver, kidney and skin wounds. However, there is growing evidence that EVs from mesenchymal stem cells may contribute to these effects and are considered a potential alternative to stem-based therapies. By exploring the effect and mechanism of hepatic ischemia–reperfusion injury (IRI), it has been revealed that human umbilical cord MSCs-EVs (huc-MSCs-EVs) can reduce the infiltration of neutrophils and reduce the oxidative stress of liver tissue in vivo, so as to protect the liver cell apoptosis induced by IRI to some extent. Further studies showed that the mechanism that EVs reduces oxidative stress is that the enzyme manganese superoxide dismutase (MnSOD) which has anti-apoptosis and anti-oxidation ability is encapsulated in the huc-MSCs-EVs [58]. In addition, huc-MSCs-EVs are proved to promote functional recovery and nerve regeneration in a study of peripheral nerve injury. It promotes the recovery of motor function and the regeneration of axon and reduced gastrocnemius atrophy [59]. Moreover, huc-MSCs-EVs ameliorated bone loss in senile osteoporotic mice. Results showed that the EVs can promote osteogenic differentiation of BMSCs and inhibit osteoclast formation of RAW264.7 cells. Meanwhile, miR-3960 mediates the osteogenesis of BMSCs by huc-MSCs-EVs [60]. At present, it has been reported that EVs ameliorates induced intrauterine adhesions with the combination of estrogen. Whether EVs were administrated alone or in combination with estrogen showed a significant reduction in inflammation and fibrosis. And the combination of EVs and estrogen showed the best therapeutic effect. Therefore, the synergistic effect of huc-MSCs-EVs and estrogen can provide a new strategy for the future clinical combination of drugs [61].

Another type of MSCs is from a peculiar structure in the umbilical cord named Wharton’s Jelly. It is a kind of gelatinous matrix that surrounds and protects the umbilical cord blood vessels [62]. At present, there have been many pieces of research about the plantation of Wharton’s Jelly MSCs(WJMSCs). However, researches on EVs from WJMSCs were fewer. Studies have discovered that Wharton-Jelly MSCs derived EVs had therapeutic effects in renal ischemia/reperfusion injury, which effect is like Wharton-Jelly MSCs [63, 64]. The study reported EVs released from WJMSCs (WJMSC-EVs) can suppress oxidation by enhancing Nrf2/ARE activation [63]. The microvesicles (hWJMSC-MVs) can enhance the proliferation and alleviate the apoptosis of renal cells. Further research showed that they also reduced the macrophages and inhibit inflammation by down-regulating CX3CL1 [64]. Another research also showed this immune function in experimental bronchopulmonary dysplasia [65]. Besides, they have neuron protective effects in perinatal brain injury through anti-inflammation [66, 67]. Therefore, it can be seen that WJMSC-EVs has wide application foreground and potential in the field.

In several studies comparing umbilical cord and bone marrow derived mesenchymal stem cell EVs, these two types of EVs were nearly identical in their physical properties and cargo content, especially for transcripts involved in immunomodulation and proliferation. The obvious difference is that Huc-MSCs produce a higher rate of EVs [68]. However, whether the ratio of components of these two types of extracellular vesicles is the same and the effect on their function remains to be further explored.

The therapeutic effects of exosomes from huc-MSCs

Exosomes derived from Huc-MSCs (HuC-MSC- Ex) have some therapeutic potentials similar to hBM-MSCs-Ex in neural restoration [68], heart repair [69], protection of liver and kidney [70, 71]. Their therapeutic effect on these diseases is related to the function of promoting angiogenesis and reducing apoptosis. For example, Li et al. reported that exosomes derived from UC-MSC (UC-MSC-Ex) can reduce hepatic inflammation and collagen deposition in liver fibrosis induced by carbon tetrachloride (CCl4) [72]. Jiang et al. and Yan et al. further discovered that UC-MSC-Ex has the function of decreasing oxidative stress and apoptosis in CCl4-Induced Liver Injury [73, 74]. At present, researchers have developed functional peptide hydrogel coated exosomes that derived from Huc-MSCs to promote heart repair. The peptide PA-GHRPS can protect H9C2 cells from H2O2-induced oxidative stress. And PGN hydrogel mixed with it and another peptide can effectively encapsulate exosomes, ensuring the stability and continuous release of exosomes. The results showed that the functional exosomes reduced inflammation, fibrosis and apoptosis, promoted angiogenesis and improved myocardial function [75]. HuC-MSC- Ex have a unique application for perinatal diseases and neonates. A study of perinatal brain injury showed that intranasally administered exosomes played a neuroprotective role in the injury. They induced a significant reduction in specific neuron cell death and promoted damaged normal myelin formation [76]. Another study showed that they can reduce nerve inflammation, as they block the degradation and proliferation of the NFκB inhibitor IκBα in response to the stimulation of phosphorylated protein kinase family of molecules LPS [66]. In a study of preterm newborn infants, researchers found that Huc-MSCs-Ex from term newborn infants had an aerobic respiration capacity independent of the entire mitochondria. And there were functional differences between term exosomes and preterm exosomes that preterm exosomes are unable to synthesize ATP. This indicated that premature babies are less able to repair damaged tissue and more able to cope with hypoxia [77]. Additionally, HuC-MSC- Ex had therapeutic effects on ovarian granulosa cell (OGC) apoptosis induced by cisplatin chemotherapy. The exosomes were successfully uptake by OGC and increased the number of living cells. Further evidence suggested that Bcl-2 and caspase-3 expressions are up-regulated, and the expressions of Bax, cleaved caspase-3 and cleaved PARP are down-regulated, indicating the protective effect of exosomes on cells [78].

In addition, some studies have pretreated exosomes such as blue light to investigate the effect on them. Under blue light exposure, HuC-MSC- Ex can promote angiogenesis in vivo and enhance its ability to promote angiogenesis. It was confirmed that the exosomes treated by blue light stimulated the activation of endothelial cells by upregulating the two miRNAs of mir-135b-5p and mir-499a-3p [79].

Compared to other sources of MSCs, exosomes from huc-MSCs have advantages in gynecological and infant diseases, but the reason remains unknown. Additionally, some external stimulation of exosomes may also promote its therapeutic effect.

The contents in huc-MSCs-Ex related to the effect

It is well known that exosomes contain small molecules that regulate cell function, including RNA and proteins. microRNAs and proteins in exosomes derived from Huc-MSCs have been further studied in many pieces of research. For example, it was found that miR-146a which was an anti-inflammatory miRNA, was strongly upregulated in TNF-α-stimulated Huc-MSCs and was enriched in exosomes. The exosome containing mir-146a was successfully uptake by fibroblasts. And it inhibited fibroblast activation and associated inflammatory responses, which may result in inhibiting urethral stricture [80]. Another research reported that the mir-146a enriched exosomes had a good effect on sepsis. The mir-146a was transferred to macrophages by huc-MSCs-Ex, resulted in M2 polarization. The increased survival in septic mice further demonstrated the efficacy of this exosome. Different from the former, the way to obtain such exosomes was pretreated Huc-MSCs with pro-inflammatory cytokines interleukin-1β (IL-1β) [81]. Moreover, in a study on treating burns, Mir-181c in huc-MSCs-Ex plays a key role in regulating inflammation. They suppressed the increase of levels of tumor necrosis factor cytokines α(TNF-α) and interleukin-1β (IL-1β) and upregulated levels of iL-10. And the over-expression of mir-181c huc-MSCs exosomes can more effectively inhibit the TLR4 signaling pathway and reduce the inflammatory response in burned rats [82]. In addition to promoting wound healing, the research has been done on whether mesenchymal stem cells promote scar formation. The results showed that Huc-MSCs reduced scarring and myofibroblast accumulation in mouse models with skin defects by huc-MSCs-Ex. High throughput RNA sequence and functional analysis revealed that specific microRNAs (Mir-21, -23a, -125b, -145) in exosomes play a key role in inhibiting myofibroblast aggregation which related to factor-β2/SMAD2 pathway [83]. Furthermore, huc-MSCs-Ex contained special protein involved in the protective effect. In previous studies, huc-MSCs-Ex were observed to have antioxidant and anti-apoptotic effects and were rescued from liver failure. A recent study revealed that glutathione peroxidase 1 (GPX1) contained in huc-MSCs-Ex, which detoxifies CCl and HO, can reduce oxidative stress and apoptosis. Therefore, huc-MSCs-ex promotes the recovery of liver oxidative injury by delivering GPX1 [74].

EVs from adipose tissue-derived mesenchymal stem cells (AD-MSCs)

The therapeutic effects of EVs from AD-MSCs

As for adipose tissue-derived mesenchymal stem cells, it is made from adipose tissue extracted from healthy adult donors or animals [55]. The main part of extracting is the donor’s abdomen and buttocks. Then special culture medium was used for filtrating MSCs [56].

EVs from human adipose tissue-derived mesenchymal stem cells (AD-MSCs) was shown to be effective in a variety of disease. Most of the functions of hAD-MSCs EVs were basically the same as those of the other two sources, such as tissue repair, reduction of injuries and anti-inflammation. Many studies have further investigated the mechanism by which EVs regulates inflammation in various disease models. In studies of osteoarthritis, EVs from AD-MSCs stimulated with interleukin- (IL-) 1β reduced the production of inflammatory mediators IL-6 and prostaglandin E2 significantly [84]. In another study, it was discovered that AD-MSCs reduced the levels of other inflammatory mediators tumor necrosis factor-α, prostaglandin E2 and NO. And the downregulation of prostaglandin E2 was caused by the reduction of cyclooxygenase-2 and microsomal prostaglandin E synthase-1. Besides, the anti-inflammatory cytokine IL-10 was upregulated, which also indicated that AD-MSCs EVs have anti-inflammatory effects [85]. Further research also showed that MMP3 was downregulated and CCL2 and CCL5 returned at pre-inflammation basal levels. CXCL8 showed significant contractions, suggesting that EVs regulates inflammation through the CREB pathway. In summary, AD-MSCs EVs decreased the expression of pro-inflammatory cytokines and chemokines in the chronic FLS inflammatory model [86]. Moreover, AD-MSCs EVs down-regulated β-galactosidase activity and the accumulation of γH2AX foci which was associated with senescence. Meanwhile, they also controlled the alteration of mitochondrial membrane potential and reduced the level of oxidative stress [84]. The anti-inflammatory effects of AD-MSCs EVs were also found in allergic asthma, with the reduction of IL-5 levels in lung tissue. However, AD-MSCs or EVs had different effects on eosinophil cell counts, levels of IL-4, IL-13, and eotaxin in lung tissue, indicating that the mechanism of anti-inflammation might be different [87].

EVs from murine AD-MSCs have also been shown to reduce neuroinflammation. Prophylactic intravenous injection of AD-MSCs nanovesicles which was 40-100 nm diameter extracellular vesicles, prior to onset significantly reduced experimental autoimmune encephalomyelitis (EAE) severity, reduced spinal cord inflammation, and demyelination [88]. And Low doses of EVs protect neurons from apoptotic cell death [89].

Studies have shown that there were differences in uptake efficiency between the EVs of BM-MSCs and AD-MSCs, but the reason for this phenomenon has not been found yet [90].

The therapeutic effects of exosomes from AD-MSCs

Exosomes derived from AD-MSCs have similar functions to other sources of exosomes, such as anti-inflammation. In a study of atopic dermatitis, AD-MSCs exosomes significantly reduced mRNA expression of various inflammatory cytokines such as interleukin (IL)-4, IL-23, IL-31, and tumor necrosis factor-α (TNF-α) in atopic dermatitis skin lesions of the mouse model [91]. The decreased inflammatory factors overlapped with those of exosomes from other sources. AD-MSCs exosomes can also modulate the polarization of macrophages by delivering miRNAs [92]. In the aspect of wound healing, AD-MSCs exosomes were absorbed and internalized by fibroblasts and increased gene expression of N-cadherin, cyclin-1, PCNA and collagen I, III. Further research found that in the early stage of wound healing, systemic administration of AD-MSCs exosomes can increase the production of type I and type III collagen. However, at the late stage of wound healing, exosomes may inhibit collagen expression to reduce scar formation [93]. Another study pointed out that AD-MSCs exosomes reduced scar formation by adjusting the ratio of collagen type III and type I [94]. At present, engineering bioactive antibacterial exosomes hydrogel named FHE@exosomes have been developed for wound therapy. The hydrogel was mainly composed of polypeptide and oxidative hyaluronic acid (OHA), which had thermal-responsive property. The hydrogel regulated the release of exosomes. Results showed that this FHE@exosomes had rapid self—healing and high antibacterial activity [95]. In addition, AD-MSCs exosomes with miR-375-overexpressing promoted bone regeneration [96]. This means that when exosomes from BM-MSCs are not suitable for treating the disease, AD-MSCs derived exosomes may be a good alternative. A recent study tried to combine AD-MSCs exosomes with a polylactic acid-glycolic acid (PLGA) scaffold to repair the skull defect and obtained certain curative effects [97].

Previous studies reported that transplanting AD-MSCs with miR-122 modification suppressed liver fibrosis [98]. Therefore, it was investigated that the therapeutic effect of exosomes derived from miR-122 modified AD-MSCs against hepatocellular carcinoma. Results showed that exosomes successfully transferred miR-122 to hepatocellular carcinoma cells and enhanced chemical sensitivity [99]. This means AD-MSCs exosomes have the potential for adjuvant chemotherapy.

Exosomes from ADSCs works in a wide and dispersed range of diseases [100], and a growing number of studies have shown that it has similar effects to exosomes from other sources of MSCs. It indicated that AD-MSCs exosomes can be an alternative when exosomes from other sources have difficulties to extract or are not suitable for therapy. However, researches of AD-MSCs exosomes are still in an early stage and therapeutic functions need to be explored further. Additionally, this source has advantages in its high availability. Therefore, exosomes from this source have a broad application prospect.

EVs from human embryonic mesenchymal stem cells (ES-MSCs)

Human embryonic mesenchymal stem cells are MSCs formed by the special induced differentiation of embryonic stem cells [101]. The proliferation and differentiation ability of ES-MSCs is weaker than that of embryonic stem cells after differentiation. Because adult mesenchymal stem cells (MSCs) exist in many tissues of the human body but are scarce and lack the pluripotent differentiation ability of embryonic stem cells, the embryonic mesenchymal stem cells have the irreplaceable advantages of the above three types of MSCs [102]. In addition, Studies showed that ES-MSCs have better immunomodulatory activity compared to BM- and AD-MSCs [103]. However, ethical issues may limit their application. And, like mesenchymal stem cells from other sources, cell rejection, ectopic tissue formation, and infusion toxicity cannot be ignored. EVs derived from ES-MSCs avoid most of the above risks and retain similar advantages to the parental MSCs.

At present, researches on ES-MSCs EVs are still at an early stage. The therapeutic effect of ES-MSCs EVs to various diseases need to explore. In a study of liver injury, Mardpour et al. showed that ES-MSCs EVs reduces liver fibrosis and collagen density. Further researches have shown that EVs upregulated apoptotic gene (BCL-2) and anti-inflammatory cytokines (TGF-β1 and IL-10) and showed immunoregulatory activity comparable to that of parental cells. These results indicated that ES-MSCs EVs ameliorated cirrhosis effectively [103]. Due to the anti-inflammatory effect, ES-MSCs EVs may have the potential therapeutic function to a variety of diseases, including allogenic skin grafts, cartilage repair and cerebral ischemia [104,105,106]. For ES-MSCs exosomes, studies revealed that they have a therapeutic effect on osteoarthritis and osteochondral defect [107, 108]. It was observed that the cartilage defect treated by ES-MSCs exosomes appeared well-formed hyaline cartilage, which completely binds to adjacent cartilage and extracellular matrix deposition that is very similar to age-matched unoperated controls [108]. Another study of osteoarthritis, exosomes from ES-MSCs promotes chondrocyte proliferation and collagen type II synthesis. Besides, they reduced the expression of inflammatory cytokines IL-1β [107].

Human embryonic mesenchymal stem cells have incomparable advantages over other sources, that is, longer telomeres, fast growth, a wider differentiation potential than adult MSCs [106]. However, Whether EVs derived from ES-MSCs are more potent in promoting proliferation and differentiation than adult MSCs remains to be further studied.

Clinical trials of the four sources of MSCs-EVs

At present, some translations of MSCs-EV based therapy entered into clinical practice (Table 1) and the data is obtained from https://clinicaltrials.gov/. Clinical trials of EVs from UC-MSCs are the most, including chronic kidney disease, macular degeneration, dry eye, and diabetes mellitus. In the trials of kidney diseases, the improvement of renal function was observed, indicating the promising clinical potential of UC-MSCs [109]. In another study of diabetes mellitus type 1 which was in phase 3, the results remained unknown. Two trials related to eye diseases are in recruiting. One ongoing trial is aimed at the dry eye in patients with cGVHD. The treatment group will receive artificial tears for 2 weeks to normalize the baseline, before using the exosomes derived from UC-MSCs. The other is to assess the safety and efficacy of exosomes from UC-MSC for promoting the healing of large and refractory macular holes. The research is in early phase 1. Currently, there are two clinical trials of EVs derived from BM-MSCs. One research is to assess the safety and efficacy of Intravenous Infusion of BM-MSCs derived EVs in preterm neonates at high risk for bronchopulmonary dysplasia. The study is in recruiting. And the clinic trial of inhalation of BM-MSCs derived exosomes to treat coronavirus is not recruiting yet. For clinical researches of AD—MSCs, they are still testing secretome from AD –MSCs, which contains many EVs. However, the effect that secretome exert doesn’t mean that it is due to EVs from AD-MSCs. Besides, there is no clinical trial of EVs from ES-MSCs, which also indicates the research of this source is still in an early stage. Above all, the current situation of clinical trials shows that BM-MSCs and UC-MSCs will be converted into clinical applications more quickly than the other two sources.

Table 1 The clinical trials of MSCs-EVs

Although clinical trials of MSCs-EVs have made some progress, there are also challenges for clinical translational studies. First, the side effects of MSCs-EVs in clinical applications are not clear. Besides, they lack standardized manufacturing processes and methods, which need to be solved in the future.

Discussion

EVs, widely found in body fluids are produced by almost all cell types. Most mature cells produce very little EVs, thus it is hard to translate applications [11]. At present, except for MSCs-EVs, EVs from tumor cells and immune cells have been studied a lot. EVs derived from tumor cells contain RNA and other content that may promote cancer [110]. Although it was reported that EVs from tumor cells were used to deliver drugs to target cancer cells [111], the risk of tumor promotion should not be ignored. EVs from immune cells such as dendritic cells and macrophages have also been studied extensively. However, conditions of cell culture are more demanding and it requires technical support related to cell-induced differentiation. Additionally, EVs from immune cells are not enough to activate potent effects in vivo [112]. The technical difficulties for transformation and applications are considerable. MSCs have an excellent capacity for proliferation and can produce a large number of EVs. Meanwhile, their risk of promoting tumor growth and metastasis is less than tumor EVs.

EVs secreted from different MSCs are proved to have effects on cardiac, kidney, liver and brain diseases and the functions have been summarized in Table 2. Their effects bear some similarity to their parental MSCs, for they carried part of the content of the parental cells. Thus, EVs are also regarded as an alternative for cell-free therapy. Compared to using MSCs directly for disease treatment, EVs reduced the risk of rejection of transplantation and variation.

Table 2 The therapeutic effects for different MSC-EV type

Although the origins of MSC-EVs are different, they have something in common. EVs derived from different sources of MSCs all have promoting angiogenesis effect. And EVs from bone marrow MSCs, human umbilical cord MSCs and human embryonic MSCs can enhance tissue repair. Except for EVs from BM-MSCs, three other kinds of MSC-EVs can suppress inflammation, but the mechanisms are a little bit different.

One of these four MSCs-EVs will show a better therapeutic effect than other kinds of EVs in certain diseases. For neurodegenerative pathologies, menstrual Mesenchymal stem cells derived exosomes enhanced neuritic outgrowth most among 4 sources MSC exosomes, including menstrual, umbilical cord, bone marrow MSCs and chorion stem cells. The research also indicated that microvesicles had opposite effects on neurons, inhibiting the growth of neurons [4]. And for glioblastoma, BM-MSCs EVs and UC-MSC EVs decreased glioblastoma cell proliferation and induced cell apoptosis, while adipose tissue MSC-EVs increased glioblastoma cell proliferation and had no effect with cell apoptosis. Experiments also showed that UC-MSC EVs performed superior effects on glioblastoma and can deliver drugs to cancer cells [21]. As for human embryonic mesenchymal stem cells, they had a more powerful neuroprotective capacity than fetal MSC-EVs [106]. However, for other diseases, which MSCs-EVs are more effective need to be further explored.

Proteomic analysis of EVs also suggested their functions. For EVs from different sources of MSCs, they contain partly similar proteins. Similar proteins account for nearly half of all proteins [113]. Additionally, it was reported that 60% of proteins were the same between EVs from human bone marrow-derived and human umbilical cord blood-derived MSCs, which were associated with cell growth and/or maintenance and anti-oxidative stress [114]. Such function-related proteins were also found in EVs from adipose tissue MSCs [115]. However, whether they are the same proteins is uncertain.

Through proteomic analysis, the mechanism of the therapeutic effect of EVs can be found. And the selective enrichment of protein of different MSCs-derived EVs indicated the difference of functions of EVs from various MSCs and the preference in Proteomic of different EVs. It partly confirms its therapeutic effect. And the effect can be changed through knockdown of specific proteins. Also, the functions of some groups of proteins can be predicted by GO analysis and this may provide directions for further research.

Conclusion and future perspective

EVs from different sources of MSC has some unique effects in some diseases and need further studies of the mechanisms. In addition, EVs are also a promising carrier, for they have better biocompatibility and intrinsic targeting ability than ordinary nanocarriers such as liposomes. Considering the specific clinical diseases, EVs from different sources can be selected and designed. Apart from exploring continually the clinical applications of MSC-EVs, we need some methods to get a large quantity of stable EVs quickly and identify the side-effect in clinical trials.

Availability of data and materials

Not applicable.

References

  1. 1.

    Lai RC, Arslan F, Lee MM, Sze NSK, Choo A, Chen TS, et al. Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury. Stem Cell Res. 2010;4(3):214–22.

    CAS  PubMed  Article  Google Scholar 

  2. 2.

    Dezawa M. Muse cells provide the pluripotency of mesenchymal stem cells: direct contribution of muse cells to tissue regeneration. Cell Transplant. 2016;25(5):849–61.

    PubMed  Article  Google Scholar 

  3. 3.

    Dalirfardouei R, Jamialahmadi K, Jafarian AH, Mahdipour E. Promising effects of exosomes isolated from menstrual blood-derived mesenchymal stem cell on wound healing process in diabetic mouse model. J Tissue Eng Regener Med. 2019;13(4):555–68.

    CAS  Article  Google Scholar 

  4. 4.

    Lopez-Verrilli MA, Caviedes A, Cabrera A, Sandoval S, Wyneken U, Khoury M. Mesenchymal stem cell-derived exosomes from different sources selectively promote neuritic outgrowth. Neuroscience. 2016;320:129–39.

    CAS  PubMed  Article  Google Scholar 

  5. 5.

    Stanko P, Altanerova U, Jakubechova J, Repiska V, Altaner C. Dental mesenchymal stem/stromal cells and their exosomes. Stem Cells Int. 2018. https://doi.org/10.1155/2018/8973613.

    Article  PubMed  PubMed Central  Google Scholar 

  6. 6.

    Bernardo ME, Fibbe WE. Mesenchymal stromal cells: sensors and switchers of inflammation. Cell Stem Cell. 2013;13(4):392–402.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  7. 7.

    Cho DI, Kim MR, Jeong HY, Jeong HC, Jeong MH, Yoon SH, et al. Mesenchymal stem cells reciprocally regulate the M1/M2 balance in mouse bone marrow-derived macrophages. Exp Mol Med. 2014;46(1):70.

    Article  CAS  Google Scholar 

  8. 8.

    Schäfer R. Advanced cell therapeutics are changing the clinical landscape: will mesenchymal stromal cells be a part of it? BMC Med. 2019;17(1):53.

    PubMed  PubMed Central  Article  Google Scholar 

  9. 9.

    Trounson A, McDonald C. Stem cell therapies in clinical trials: progress and challenges. Cell Stem Cell. 2015;17(1):11–22.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  10. 10.

    da Silva Meirelles L, Fontes AM, Covas DT, Caplan AI. Mechanisms involved in the therapeutic properties of mesenchymal stem cells. Cytokine Growth Factor Rev. 2009;20(5–6):419–27.

    Google Scholar 

  11. 11.

    Van Niel G, d’Angelo G, Raposo G. Shedding light on the cell biology of extracellular vesicles. Nat Rev Mol Cell Biol. 2018;19(4):213–28.

    PubMed  Article  CAS  Google Scholar 

  12. 12.

    Katsuda T, Kosaka N, Takeshita F, Ochiya T. The therapeutic potential of mesenchymal stem cell-derived extracellular vesicles. Proteomics. 2013;13(10–11):1637–53.

    CAS  PubMed  Article  Google Scholar 

  13. 13.

    Théry C, Witwer KW, Aikawa E, Alcaraz MJ, Anderson JD, Andriantsitohaina R, et al. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for extracellular vesicles and update of the MISEV2014 guidelines. J Extracellular Vesicles. 2018;7(1):1535750.

    Article  Google Scholar 

  14. 14.

    Lee Y, El Andaloussi S, Wood MJ. Exosomes and microvesicles: extracellular vesicles for genetic information transfer and gene therapy. Hum Mol Genet. 2012;21(R1):R125–34.

    CAS  PubMed  Article  Google Scholar 

  15. 15.

    Phinney DG, Pittenger MF. Concise review: MSC-derived exosomes for cell-free therapy. Stem Cells. 2017;35(4):851–8.

    CAS  Article  PubMed  Google Scholar 

  16. 16.

    Ge M, Ke R, Cai T, Yang J, Mu X. Identification and proteomic analysis of osteoblast-derived exosomes. Biochem Biophys Res Commun. 2015;467(1):27–32.

    CAS  PubMed  Article  Google Scholar 

  17. 17.

    Hessvik NP, Llorente A. Current knowledge on exosome biogenesis and release. Cell Mol Life Sci. 2018;75(2):193–208.

    CAS  PubMed  Article  Google Scholar 

  18. 18.

    Wolf P. The nature and significance of platelet products in human plasma. Br J Haematol. 1967;13(3):269–88.

    CAS  PubMed  Article  Google Scholar 

  19. 19.

    Simons M, Raposo G. Exosomes—vesicular carriers for intercellular communication. Curr Opin Cell Biol. 2009;21(4):575–81.

    CAS  PubMed  Article  Google Scholar 

  20. 20.

    Huang Y, Liu K, Li Q, Yao Y, Wang Y. Exosomes function in tumor immune microenvironment. Exosomes Stem Cells and MicroRNA: Springer; 2018. p. 109–22.

    Google Scholar 

  21. 21.

    Del Fattore A, Luciano R, Saracino R, Battafarano G, Rizzo C, Pascucci L, et al. Differential effects of extracellular vesicles secreted by mesenchymal stem cells from different sources on glioblastoma cells. Expert Opin Biol Ther. 2015;15(4):495–504.

    PubMed  Article  CAS  Google Scholar 

  22. 22.

    García-Romero N, Carrión-Navarro J, Esteban-Rubio S, Lázaro-Ibáñez E, Peris-Celda M, Alonso MM, et al. DNA sequences within glioma-derived extracellular vesicles can cross the intact blood-brain barrier and be detected in peripheral blood of patients. Oncotarget. 2017;8(1):1416.

    PubMed  Article  Google Scholar 

  23. 23.

    Zhang Y, Chopp M, Meng Y, Katakowski M, Xin H, Mahmood A, et al. Effect of exosomes derived from multipluripotent mesenchymal stromal cells on functional recovery and neurovascular plasticity in rats after traumatic brain injury. J Neurosurg. 2015;122(4):856–67.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  24. 24.

    Bian S, Zhang L, Duan L, Wang X, Min Y, Yu H. Extracellular vesicles derived from human bone marrow mesenchymal stem cells promote angiogenesis in a rat myocardial infarction model. J Mol Med. 2014;92(4):387–97.

    CAS  PubMed  Article  Google Scholar 

  25. 25.

    De Bari C, Roelofs AJ. Stem cell-based therapeutic strategies for cartilage defects and osteoarthritis. Current Opin Pharmacol. 2018;40:74–80.

    Article  CAS  Google Scholar 

  26. 26.

    Vonk LA, van Dooremalen SFJ, Liv N, Klumperman J, Coffer PJ, Saris DBF, Lorenowicz MJ. Mesenchymal stromal/stem cell-derived extracellular vesicles promote human cartilage regeneration in vitro. Theranostics. 2018;8(4):906–20.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  27. 27.

    Borgovan T, Crawford L, Nwizu C, Quesenberry P. Stem cells and extracellular vesicles: biological regulators of physiology and disease. Am J Physiol Cell Physiol. 2019;317(2):C155–66.

    CAS  PubMed  Article  Google Scholar 

  28. 28.

    Martins M, Ribeiro D, Martins A, Reis RL, Neves NM. Extracellular vesicles derived from osteogenically induced human bone marrow mesenchymal stem cells can modulate lineage commitment. Stem cell Rep. 2016;6(3):284–91.

    CAS  Article  Google Scholar 

  29. 29.

    Qin Y, Wang L, Gao Z, Chen G, Zhang C. Bone marrow stromal/stem cell-derived extracellular vesicles regulate osteoblast activity and differentiation in vitro and promote bone regeneration in vivo. Sci Rep. 2016;6:21961.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  30. 30.

    Shi Z, Wang Q, Jiang D. Extracellular vesicles from bone marrow-derived multipotent mesenchymal stromal cells regulate inflammation and enhance tendon healing. J Transl Med. 2019;17(1):211.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  31. 31.

    Matsukura T, Inaba C, Weygant EA, Kitamura D, Janknecht R, Matsumoto H, et al. Extracellular vesicles from human bone marrow mesenchymal stem cells repair organ damage caused by cadmium poisoning in a medaka model. Physiol Rep. 2019;7(14):14172.

    Article  CAS  Google Scholar 

  32. 32.

    Bruno S, Tapparo M, Collino F, Chiabotto G, Deregibus MC, Soares Lindoso R, et al. Renal regenerative potential of different extracellular vesicle populations derived from bone marrow mesenchymal stromal cells. Tissue Eng Part A. 2017;23:1262–73.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  33. 33.

    Grange C, Tritta S, Tapparo M, Cedrino M, Tetta C, Camussi G, et al. Stem cell-derived extracellular vesicles inhibit and revert fibrosis progression in a mouse model of diabetic nephropathy. Sci Rep. 2019;9(1):1–3.

    CAS  Article  Google Scholar 

  34. 34.

    Hyvärinen K, Holopainen M, Skirdenko V, Ruhanen H, Lehenkari P, Korhonen M, et al. Mesenchymal stromal cells and their extracellular vesicles enhance the anti-inflammatory phenotype of regulatory macrophages by downregulating the production of interleukin (IL)-23 and IL-22. Front mmunol. 2018;9:771.

    Article  CAS  Google Scholar 

  35. 35.

    Fujii S, Miura Y, Fujishiro A, Shindo T, Shimazu Y, Hirai H, et al. Graft-versus-host disease amelioration by human bone marrow mesenchymal stromal/stem cell-derived extracellular vesicles is associated with peripheral preservation of naive T cell populations. Stem cells. 2018;36(3):434–45.

    CAS  PubMed  Article  Google Scholar 

  36. 36.

    Del Fattore A, Luciano R, Pascucci L, Goffredo BM, Giorda E, Scapaticci M, et al. Immunoregulatory effects of mesenchymal stem cell-derived extracellular vesicles on T lymphocytes. Cell Trans. 2015;24(12):2615–27.

    Article  Google Scholar 

  37. 37.

    Haga H, Yan IK, Takahashi K, Matsuda A, Patel T. extracellular vesicles from bone marrow-derived mesenchymal stem cells improve survival from lethal hepatic failure in mice. Stem cells Transl Med. 2017;6(4):1262–72.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  38. 38.

    Elia CA, Tamborini M, Rasile M, Desiato G, Marchetti S, Swuec P, et al. Intracerebral injection of extracellular vesicles from mesenchymal stem cells exerts reduced a beta plaque burden in early stages of a preclinical model of alzheimer’s disease. Cells. 2019;8(9):1059.

    CAS  PubMed Central  Article  Google Scholar 

  39. 39.

    Schoefinius JS, Brunswig-Spickenheier B, Speiseder T, Krebs S, Just U, Lange C. Mesenchymal stromal cell-derived extracellular vesicles provide long-term survival after total body irradiation without additional hematopoietic stem cell support. Stem Cells. 2017;35(12):2379–89.

    CAS  PubMed  Article  Google Scholar 

  40. 40.

    Cruz FF, Borg ZD, Goodwin M, Sokocevic D, Wagner DE, Coffey A, et al. Systemic administration of human bone marrow-derived mesenchymal stromal cell extracellular vesicles ameliorates aspergillus hyphal extract-induced allergic airway inflammation in immunocompetent mice. Stem cells Transl Med. 2015;4(11):1302–16.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  41. 41.

    Rong X, Liu J, Yao X, Jiang T, Wang Y, Xie F. Human bone marrow mesenchymal stem cells-derived exosomes alleviate liver fibrosis through the Wnt/β-catenin pathway. Stem cell Res Ther. 2019;10(1):98.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  42. 42.

    Perets N, Hertz S, London M, Offen D. Intranasal administration of exosomes derived from mesenchymal stem cells ameliorates autistic-like behaviors of BTBR mice. Mol Autism. 2018;9:57.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  43. 43.

    Khare D, Or R, Resnick I, Barkatz C, Almogi-Hazan O, Avni B. Mesenchymal stromal cell-derived exosomes affect mRNA expression and function of B-lymphocytes. Front Immunol. 2018;9:3053.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  44. 44.

    He X, Dong Z, Cao Y, Wang H, Liu S, Liao L, et al. MSC-derived exosome promotes M2 polarization and enhances cutaneous wound healing. Stem cells Int. 2019. https://doi.org/10.1155/2019/7132708.

    Article  PubMed  PubMed Central  Google Scholar 

  45. 45.

    Xu R, Zhang F, Chai R, Zhou W, Hu M, Liu B, et al. Exosomes derived from pro-inflammatory bone marrow-derived mesenchymal stem cells reduce inflammation and myocardial injury via mediating macrophage polarization. J Cell Mol Med. 2019;23(11):7617–31.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  46. 46.

    Li Z, Liu F, He X, Yang X, Shan F, Feng J. Exosomes derived from mesenchymal stem cells attenuate inflammation and demyelination of the central nervous system in EAE rats by regulating the polarization of microglia. Int Immunopharmacol. 2019;67:268–80.

    CAS  PubMed  Article  Google Scholar 

  47. 47.

    He JG, Li HR, Han JX, Li BB, Yan D, Li HY, Wang P, Luo Y, et al. GATA-4-expressing mouse bone marrow mesenchymal stem cells improve cardiac function after myocardial infarction via secreted exosomes. Sci Rep. 2018;8(1):9047.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  48. 48.

    Wang Y, Zhao R, Liu D, Deng W, Xu G, Liu W, Rong J, Long X, Ge J, Shi B, et al. Exosomes derived from miR-214-enriched bone marrow-derived mesenchymal stem cells regulate oxidative damage in cardiac stem cells by targeting CaMKII. Oxidative Med Cellular Longevity. 2018;2018:4971261.

    Google Scholar 

  49. 49.

    Zhu J, Lu K, Zhang N, Zhao Y, Ma Q, Shen J, et al. Myocardial reparative functions of exosomes from mesenchymal stem cells are enhanced by hypoxia treatment of the cells via transferring microRNA-210 in an nSMase2-dependent way. Artificial Cells Nanomed Biotechnol. 2018;46(8):1659–70.

    CAS  Google Scholar 

  50. 50.

    Wu H, Fan H, Shou Z, Xu M, Chen Q, Ai C, et al. Extracellular vesicles containing miR-146a attenuate experimental colitis by targeting TRAF6 and IRAK1. Int Immunopharmacol. 2019;68:204–12.

    CAS  PubMed  Article  Google Scholar 

  51. 51.

    Xu HJ, Liao W, Liu XZ, Hu J, Zou WZ, Ning Y, Yang Y, Li ZH, et al. Down-regulation of exosomal microRNA-224-3p derived from bone marrow-derived mesenchymal stem cells potentiates angiogenesis in traumatic osteonecrosis of the femoral head. FASEB J. 2019;33(7):8055–68.

    CAS  PubMed  Article  Google Scholar 

  52. 52.

    Zhu G, Pei L, Lin F, Yin H, Li X, He W, Liu N, Gou X, et al. Exosomes from human-bone-marrow-derived mesenchymal stem cells protect against renal ischemia/reperfusion injury via transferring miR-199a-3p. J Cell Physiol. 2019;234(12):23736–49.

    CAS  PubMed  Article  Google Scholar 

  53. 53.

    Wang C, Zhu G, He W, Yin H, Lin F, Gou X, Li X, et al. BMSCs protect against renal ischemia-reperfusion injury by secreting exosomes loaded with miR-199a-5p that target BIP to inhibit endoplasmic reticulum stress at the very early reperfusion stages. FASEB J. 2019;33(4):5440–56.

    CAS  PubMed  Article  Google Scholar 

  54. 54.

    Su T, Xiao Y, Xiao YE, Guo Q, Li C, Huang Y, et al. Bone marrow mesenchymal stem cells-derived exosomal MiR-29b-3p regulates aging-associated insulin resistance. ACS Nano. 2019;13(2):2450–62.

    CAS  PubMed  Google Scholar 

  55. 55.

    Pascucci L, Coccè V, Bonomi A, Ami D, Ceccarelli P, Ciusani E, et al. Paclitaxel is incorporated by mesenchymal stromal cells and released in exosomes that inhibit in vitro tumor growth: a new approach for drug delivery. J Control Release. 2014;192:262–70.

    CAS  PubMed  Article  Google Scholar 

  56. 56.

    Gomari H, Moghadam MF, Soleimani M, Ghavami M, Khodashenas S. Targeted delivery of doxorubicin to HER2 positive tumor models. Int J Nanomed. 2019;14:5679–90.

    CAS  Article  Google Scholar 

  57. 57.

    Lv K, Li Q, Zhang L, Wang Y, Zhong Z, Zhao J, et al. Incorporation of small extracellular vesicles in sodium alginate hydrogel as a novel therapeutic strategy for myocardial infarction. Theranostics. 2019;9(24):7403–16.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  58. 58.

    Yao J, Zheng J, Cai J, Zeng K, Zhou C, Zhang J, et al. Extracellular vesicles derived from human umbilical cord mesenchymal stem cells alleviate rat hepatic ischemia-reperfusion injury by suppressing oxidative stress and neutrophil inflammatory response. FASEB J. 2019;33(2):1695–710.

    CAS  PubMed  Article  Google Scholar 

  59. 59.

    Ma Y, Dong L, Zhou D, Li L, Zhang W, Zhen Y, et al. Extracellular vesicles from human umbilical cord mesenchymal stem cells improve nerve regeneration after sciatic nerve transection in rats. J Cell Mol Med. 2019;23(4):2822–35.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  60. 60.

    Hu Y, Xu R, Chen CY, Rao SS, Xia K, Huang J, et al. Extracellular vesicles from human umbilical cord blood ameliorate bone loss in senile osteoporotic mice. Metabolism. 2019;1(95):93–101.

    Article  CAS  Google Scholar 

  61. 61.

    Ebrahim N, Mostafa O, El Dosoky RE, Ahmed IA, Saad AS, Mostafa A, et al. Human mesenchymal stem cell-derived extracellular vesicles/estrogen combined therapy safely ameliorates experimentally induced intrauterine adhesions in a female rat model. Stem Cell Res Ther. 2018;9(1):175.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  62. 62.

    Zeddou M, Briquet A, Relic B, Josse C, Malaise MG, Gothot A, et al. The umbilical cord matrix is a better source of mesenchymal stem cells (MSC) than the umbilical cord blood. Cell Biol Int. 2010;34(7):693–701.

    PubMed  Article  Google Scholar 

  63. 63.

    Zhang G, Zou X, Huang Y, Wang F, Miao S, Liu G, et al. Mesenchymal stromal cell-derived extracellular vesicles protect against acute kidney injury through anti-oxidation by enhancing Nrf2/ARE activation in rats. Kidney Blood Pressure Res. 2016;41(2):119–28.

    Article  Google Scholar 

  64. 64.

    Zou X, Zhang G, Cheng Z, Yin D, Du T, Ju G, et al. Microvesicles derived from human Wharton’s Jelly mesenchymal stromal cells ameliorate renal ischemia-reperfusion injury in rats by suppressing CX3CL1. Stem Cell Res Ther. 2014;5(2):40.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  65. 65.

    Willis GR, Fernandez-Gonzalez A, Anastas J, Vitali SH, Liu X, Ericsson M, et al. Mesenchymal stromal cell exosomes ameliorate experimental bronchopulmonary dysplasia and restore lung function through macrophage immunomodulation. Am J Respir Crit Care Med. 2018;197(1):104–16.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  66. 66.

    Thomi G, Surbek D, Haesler V, Joerger-Messerli M, Schoeberlein A. Exosomes derived from umbilical cord mesenchymal stem cells reduce microglia-mediated neuroinflammation in perinatal brain injury. Stem Cell Res Ther. 2019;10:105.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  67. 67.

    Joerger-Messerli MS, Oppliger B, Spinelli M, Thomi G, di Salvo I, Schneider P, et al. Extracellular vesicles derived from wharton’s jelly mesenchymal stem cells prevent and resolve programmed cell death mediated by perinatal hypoxia-ischemia in neuronal cells. Cell Trans. 2018;27(1):168–80.

    Article  Google Scholar 

  68. 68.

    Ragni E, Banfi F, Barilani M, Cherubini A, Parazzi V, Larghi P, et al. Extracellular vesicle-shuttled mRNA in mesenchymal stem cell communication. Stem cells. 2017;35(4):1093–105.

    CAS  PubMed  Article  Google Scholar 

  69. 69.

    Ma J, Zhao Y, Sun L, Sun X, Zhao X, Sun X, et al. Exosomes derived from akt-modified human umbilical cord mesenchymal stem cells improve cardiac regeneration and promote angiogenesis via activating platelet-derived growth factor D. Stem cells Transl Med. 2017;6(1):51–9.

    CAS  PubMed  Article  Google Scholar 

  70. 70.

    Zhang L, Song Y, Chen L, Li D, Feng H, Lu Z, et al. MiR-20a-containing exosomes from umbilical cord mesenchymal stem cells alleviates liver ischemia/reperfusion injury. J Cell Physiol. 2020;235(4):3698–710.

    CAS  PubMed  Article  Google Scholar 

  71. 71.

    Wang B, Jia H, Zhang B, Wang J, Ji C, Zhu X, et al. Pre-incubation with hucMSC-exosomes prevents cisplatin-induced nephrotoxicity by activating autophagy. Stem cell Res Ther. 2017;8(1):75.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  72. 72.

    Li T, Yan Y, Wang B, Qian H, Zhang X, Shen L, et al. Exosomes derived from human umbilical cord mesenchymal stem cells alleviate liver fibrosis. Stem Cells Dev. 2012;22(6):845–54.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  73. 73.

    Jiang W, Tan Y, Cai M, Zhao T, Mao F, Zhang X, et al. Human umbilical cord MSC-derived exosomes suppress the development of CCl4-induced liver injury through antioxidant effect. Stem Cells Int. 2018. https://doi.org/10.1155/2018/6079642.

    Article  PubMed  PubMed Central  Google Scholar 

  74. 74.

    Yan Y, Jiang W, Tan Y, Zou S, Zhang H, Mao F, et al. hucMSC Exosome-Derived GPX1 Is required for the recovery of hepatic oxidant injury. Mol Ther J Am Soc Gene Ther. 2017;25(2):465–79.

    CAS  Article  Google Scholar 

  75. 75.

    Han C, Zhou J, Liang C, Liu B, Pan X, Zhang Y, et al. Human umbilical cord mesenchymal stem cell derived exosomes encapsulated in functional peptide hydrogels promote cardiac repair. Biomat Sci. 2019;7(7):2920–33.

    CAS  Article  Google Scholar 

  76. 76.

    Thomi G, Joerger-Messerli M, Haesler V, Muri L, Surbek D, Schoeberlein A. Intranasally administered exosomes from umbilical cord stem cells have preventive neuroprotective effects and contribute to functional recovery after perinatal brain injury. Cells. 2019;8(8):855.

    CAS  PubMed Central  Article  Google Scholar 

  77. 77.

    Panfoli I, Ravera S, Podesta M, Cossu C, Santucci L, Bartolucci M, Bruschi M, et al. Exosomes from human mesenchymal stem cells conduct aerobic metabolism in term and preterm newborn infants. FASEB J. 2016;30(4):1416–24.

    CAS  PubMed  Article  Google Scholar 

  78. 78.

    Sun L, Li D, Song K, Wei J, Yao S, Li Z, et al. Exosomes derived from human umbilical cord mesenchymal stem cells protect against cisplatin-induced ovarian granulosa cell stress and apoptosis in vitro. Sci Rep. 2017;7(1):2552.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  79. 79.

    Yang K, Li D, Wang M, Xu Z, Chen X, Liu Q, et al. Exposure to blue light stimulates the proangiogenic capability of exosomes derived from human umbilical cord mesenchymal stem cells. Stem Cell Res Ther. 2019;10(1):358.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  80. 80.

    Yc L, Yp W, Xd L, Sh C, Xj Y, Xy X, et al. TNF-α-induced exosomal miR-146a mediates mesenchymal stem cell-dependent suppression of urethral stricture. J Cell Physiol. 2019;234(12):23243–55.

    Article  CAS  Google Scholar 

  81. 81.

    Song Y, Dou H, Li X, Zhao X, Li Y, Liu D, Ji J, Liu F, Ding L, et al. Exosomal miR-146a contributes to the enhanced therapeutic efficacy of interleukin-1β-primed mesenchymal stem cells against sepsis. Stem cells. 2017;35(5):1208–21.

    CAS  PubMed  Article  Google Scholar 

  82. 82.

    Li X, Liu L, Yang J, Yu Y, Chai J, Wang L, et al. Exosome derived from human umbilical cord mesenchymal stem cell mediates MiR-181c attenuating burn-induced excessive inflammation. EBioMedicine. 2016;1(8):72–82.

    Article  Google Scholar 

  83. 83.

    Fang S, Xu C, Zhang Y, Xue C, Yang C, Bi H, et al. Umbilical cord-derived mesenchymal stem cell-derived exosomal micrornas suppress myofibroblast differentiation by inhibiting the transforming growth factor-β/SMAD2 pathway during wound healing. Stem cells Transl Med. 2016;5(10):1425–39.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  84. 84.

    Tofiño-Vian M, Guillén MI, del Caz P, Dolores M, Castejón MA, Alcaraz MJ. Extracellular vesicles from adipose-derived mesenchymal stem cells downregulate senescence features in osteoarthritic osteoblasts. Oxidative Med Cell Longevity. 2017. https://doi.org/10.1155/2017/7197598.

    Article  Google Scholar 

  85. 85.

    Tofiño-Vian M, Guillén MI, del Caz MD, Silvestre A, Alcaraz MJ. Microvesicles from human adipose tissue-derived mesenchymal stem cells as a new protective strategy in osteoarthritic chondrocytes. Cell Physiol Biochem. 2018;47(1):11–25.

    PubMed  Article  CAS  Google Scholar 

  86. 86.

    Ragni E, Orfei CP, De Luca P, Lugano G, Viganò M, Colombini A, et al. Interaction with hyaluronan matrix and miRNA cargo as contributors for in vitro potential of mesenchymal stem cell-derived extracellular vesicles in a model of human osteoarthritic synoviocytes. Stem Cell ResTher. 2019;10(1):109.

    CAS  Google Scholar 

  87. 87.

    de Castro LL, Xisto DG, Kitoko JZ, Cruz FF, Olsen PC, Redondo PAG, et al. Human adipose tissue mesenchymal stromal cells and their extracellular vesicles act differentially on lung mechanics and inflammation in experimental allergic asthma. Stem cell Research Therapy. 2017;8(1):151.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  88. 88.

    A F, S A, E T, E B, S D, S R, et al. Nanovesicles from adipose-derived mesenchymal stem cells inhibit T lymphocyte trafficking and ameliorate chronic experimental autoimmune encephalomyelitis. Scientific reports. 2018;8(1):7473.

  89. 89.

    A F, E T, S M, E B, E B, B B. Murine adipose-derived mesenchymal stromal cell vesicles: in vitro clues for neuroprotective and neuroregenerative approaches. Cytotherapy. 2015;17(5):571-8.

    CAS  PubMed  Article  Google Scholar 

  90. 90.

    A G, R K, S K, S M, S H, H P, et al. Monocyte-derived extracellular vesicles stimulate cytokine secretion and gene expression of matrix metalloproteinases by mesenchymal stem/stromal cells. The FEBS journal. 2018;285(12):2337-59.

    CAS  PubMed  Article  Google Scholar 

  91. 91.

    Bs C, Jo K, Dh H, Yw Y. Exosomes derived from human adipose tissue-derived mesenchymal stem cells alleviate atopic dermatitis. Stem cell research & therapy. 2018;9(1):187.

    Article  CAS  Google Scholar 

  92. 92.

    Domenis R, Cifù A, Quaglia S, Pistis C, Moretti M, Vicario A, et al. Pro inflammatory stimuli enhance the immunosuppressive functions of adipose mesenchymal stem cells-derived exosomes. Sci Rep. 2018;8(1):1–1.

    CAS  Article  Google Scholar 

  93. 93.

    Hu L, Wang J, Zhou X, Xiong Z, Zhao J, Yu R, et al. Exosomes derived from human adipose mensenchymal stem cells accelerates cutaneous wound healing via optimizing the characteristics of fibroblasts. Sci Rep. 2016;12(6):32993.

    Article  CAS  Google Scholar 

  94. 94.

    Wang L, Hu L, Zhou X, Xiong Z, Zhang C, Shehada HM, et al. Exosomes secreted by human adipose mesenchymal stem cells promote scarless cutaneous repair by regulating extracellular matrix remodelling. Sci Rep. 2017;7(1):1–2.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  95. 95.

    Wang C, Wang M, Xu T, Zhang X, Lin C, Gao W, et al. Engineering bioactive self-healing antibacterial exosomes hydrogel for promoting chronic diabetic wound healing and complete skin regeneration. Theranostics. 2019;9(1):65.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  96. 96.

    Chen S, Tang Y, Liu Y, Zhang P, Lv L, Zhang X, et al. Exosomes derived from miR-375-overexpressing human adipose mesenchymal stem cells promote bone regeneration. Cell Proliferation. 2019;52(5):e12669.

    PubMed  PubMed Central  Google Scholar 

  97. 97.

    Li W, Liu Y, Zhang P, Tang Y, Zhou M, Jiang W, Zhang X, Wu G, Zhou Y. Tissue-engineered bone immobilized with human adipose stem cells-derived exosomes promotes bone regeneration. ACS Appl Mater Interfaces. 2018;10(6):5240–54.

    CAS  Article  PubMed  Google Scholar 

  98. 98.

    Lou G, Yang Y, Liu F, Ye B, Chen Z, Zheng M, et al. MiR-122 modification enhances the therapeutic efficacy of adipose tissue-derived mesenchymal stem cells against liver fibrosis. J Cell Mol Med. 2017;21(11):2963–73.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  99. 99.

    Lou G, Song X, Yang F, Wu S, Wang J, Chen Z, et al. Exosomes derived from miR-122-modified adipose tissue-derived MSCs increase chemosensitivity of hepatocellular carcinoma. J Hematol Oncol. 2015;8(1):122.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  100. 100.

    Hong P, Yang H, Wu Y, Li K, Tang Z. The functions and clinical application potential of exosomes derived from adipose mesenchymal stem cells: a comprehensive review. Stem Cell Res Ther. 2019;10(1):242.

    PubMed  PubMed Central  Article  Google Scholar 

  101. 101.

    Olivier EN, Rybicki AC, Bouhassira EE. Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells. Stem cells. 2006;24(8):1914–22.

    CAS  PubMed  Article  Google Scholar 

  102. 102.

    Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284(5411):143-7.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  103. 103.

    Mardpour S, Hassani S-N, Mardpour S, Sayahpour F, Vosough M, Ai J, et al. Extracellular vesicles derived from human embryonic stem cell-MSCs ameliorate cirrhosis in thioacetamide-induced chronic liver injury. J Cell Physiol. 2018;233(12):9330–44.

    CAS  PubMed  Article  Google Scholar 

  104. 104.

    Zhang S, Chuah SJ, Lai RC, Hui JHP, Lim SK, Toh WS. MSC exosomes mediate cartilage repair by enhancing proliferation, attenuating apoptosis and modulating immune reactivity. Biomaterials. 2018;156:16–27.

    CAS  PubMed  Article  Google Scholar 

  105. 105.

    Zhang B, Yin Y, Lai RC, Tan SS, Choo ABH, Lim SK. Mesenchymal stem cells secrete immunologically active exosomes. Stem Cells Dev. 2013;23(11):1233–44.

    Article  CAS  Google Scholar 

  106. 106.

    Hawkins KE, Corcelli M, Dowding K, Ranzoni AM, Vlahova F, Hau KL, et al. Embryonic stem cell-derived mesenchymal stem cells (MSCs) have a superior neuroprotective capacity over fetal MSCs in the hypoxic-ischemic mouse brain. Stem Cells Transl Med. 2018;7(5):439–49.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  107. 107.

    Wang YF, Yu DS, Liu ZM, Zhou F, Dai J, Wu BB, et al. Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix. Stem Cell Res Ther. 2017;8:13.

    Article  Google Scholar 

  108. 108.

    Zhang S, Chu W, Lai R, Lim S, Hui J, Toh W. Exosomes derived from human embryonic mesenchymal stem cells promote osteochondral regeneration. Osteoarthr Cartilage. 2016;24(12):2135–40.

    CAS  Article  Google Scholar 

  109. 109.

    Nassar W, El-Ansary M, Sabry D, Mostafa MA, Fayad T, Kotb E, et al. Umbilical cord mesenchymal stem cells derived extracellular vesicles can safely ameliorate the progression of chronic kidney diseases. Biomater Res. 2016;20(1):21.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  110. 110.

    Tai YL, Chu PY, Lee BH, Chen KC, Yang CY, Kuo WH, Shen TL. Basics and applications of tumor-derived extracellular vesicles. J Biomed Sci. 2019;26(1):35.

    PubMed  PubMed Central  Article  Google Scholar 

  111. 111.

    Yj L, Jy W, Jm W, Xb H, Jx C, Dx X. Gemcitabine loaded autologous exosomes for effective and safe chemotherapy of pancreatic cancer. Acta Biomater. 2020;101:519–30.

    Article  CAS  Google Scholar 

  112. 112.

    Veerman RE, Akpinar GG, Eldh M, Gabrielsson S. Immune cell-derived extracellular vesicles–functions and therapeutic applications. Trends Mol Med. 2019;25(5):382–94.

    CAS  PubMed  Article  Google Scholar 

  113. 113.

    van Balkom BW, Gremmels H, Giebel B, Lim SK. Proteomic signature of mesenchymal stromal cell-derived small extracellular vesicles. Proteomics. 2019;19(1–2):1800163.

    Article  CAS  Google Scholar 

  114. 114.

    Angulski AB, Capriglione LG, Batista M, Marcon BH, Senegaglia AC, Stimamiglio MA, et al. The protein content of extracellular vesicles derived from expanded human umbilical cord blood-derived CD133 + and human bone marrow-derived mesenchymal stem cells partially explains why both sources are advantageous for regenerative medicine. Stem Cell Rev Rep. 2017;13(2):244–57.

    CAS  PubMed  Article  Google Scholar 

  115. 115.

    Eirin A, Zhu X-Y, Puranik AS, Woollard JR, Tang H, Dasari S, et al. Comparative proteomic analysis of extracellular vesicles isolated from porcine adipose tissue-derived mesenchymal stem/stromal cells. Sci Rep. 2016;6:36120.

    PubMed  PubMed Central  Article  Google Scholar 

Download references

Acknowledgements

Not applicable.

Funding

Not applicable.

Author information

Affiliations

Authors

Contributions

CJ and LS formulated the idea. WJ made the figure and the table. LY, HX and WJ critically revised the manuscript. CJ wrote the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Daxiong Xiang.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Cai, J., Wu, J., Wang, J. et al. Extracellular vesicles derived from different sources of mesenchymal stem cells: therapeutic effects and translational potential. Cell Biosci 10, 69 (2020). https://doi.org/10.1186/s13578-020-00427-x

Download citation

Keywords

  • Extracellular vesicles
  • Exosomes
  • Mesenchymal stem cells