Antimicrobial activity of TAT-bNK2A and bNK2A against intracellular M. paratuberculosis. (A) Mean percentages of live and dead intracellular MAP in MDMs with the corresponding standard error of the mean. Representative confocal laser scanning microscopy images of live (green) and dead (red) intracellular MAP in (B) control (C) 10 µM bNK2A and (D) 10 µM TAT-bNK2A peptide-treated MDMs. Large red circles within MDMs are PI-stained nuclei. The identification of MDMs in each well was confirmed by positive labelling with anti-CD68 antibody (orange colour cells, B, C and D). Selected MDMs (small white boxes) in each panel were digitally enlarged and are shown in the upper right corner (large white boxes). Means and SEMs of live and dead MAP in infected MDMs were calculated from four cows. (Scale bar = 2 µm). P = 0.6923.