Fig. 2 | Diabetology & Metabolic Syndrome

Fig. 2

From: Experimental dopaminergic neuron lesion at the area of the biological clock pacemaker, suprachiasmatic nuclei (SCN) induces metabolic syndrome in rats

Fig. 2

Daily profiles of extracellular dopamine, 3,4-dihydroxyphenylacetic acid, and homovanillic acid concentration (pg/μl) in microdialysate samples from the peri-SCN of freely-living rats (mean ± SEM, n = 8/group). Animals received either an infusion of 6-OHDA (2 or 8 μg/SCN side) or vehicle at the peri-SCN area bilaterally. Microdialysis experimentation was conducted at 12—20 days after lesion and samples were collected for each of 5 sequential 30 min intervals. The horizontal bar indicates light and dark phases of the time period samples were collected. a a two-way ANOVA with repeated measures on extracellular dopamine (DA) indicates an overall group effect (F2, 21 = 3.759, P < 0.05); Post hoc Duncan test shows a significant reduced extracellular DA in animals with each dose of 6-OHDA lesion compared to sham lesion. b, c Two-way ANOVA with repeated measures on extracellular DOPAC (b) and HVA (c) in peri-SCN reveals a significant group effect (F2, 21 = 5.291, F2, 21 = 5.378 respectively, P < 0.05). Both high and low 6-OHDA lesion markedly reduced extracellular DOPAC and HVA concentration at the peri-SCN. There was no difference in effect between low and high 6-OHDA dose lesion groups

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