Fig. 1 | Diabetology & Metabolic Syndrome

Fig. 1

From: Experimental dopaminergic neuron lesion at the area of the biological clock pacemaker, suprachiasmatic nuclei (SCN) induces metabolic syndrome in rats

Fig. 1

Histology of SCN at 8 weeks post infusion of 6-OHDA. The external cannula tip location was at the stereotaxic coordinates of anteroposterior−1.3, mediolateral 0.4 mm and dorsoventral−7.4 mm from dura. The injection cannula protruded 2 mm from the guide cannula landing lateral to the perimeter edge of the SCN. A representative picture of a peri-SCN coronal section from a dopamine neuron lesioned (4 µg/side 6-OHDA plus protriptyline 20 mg/kg ip) rat is shown stained with cresyl violet with placement of the infusion cannula (a). The arrows indicate the infusion sites just outside the SCN (peri-SCN area). b An enlarged micrograph of the same histology section of (a) of the peri-SCN and SCN regions. The cresyl violet staining demonstrates normal neuronal anatomy with no neuronal damage. c Diagrammatic representation of the peri-SCN injection target site. The 6-OHDA is taken up by dopamine neuron terminals in the peri-SCN region and transported back to the cell body where it is toxic to the cell

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