A developing Setaria viridis internode: an experimental system for the study of biomass generation in a C4 model species
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Recently, there has been interest in establishing a monocot C4 model species with a small genome, short lifecycle, and capacity for genetic transformation. Setaria viridis has been adopted to fill this role, since reports of Agrobacterium-mediated transformation in 2010, and sequencing of its genome in 2012. To date, S. viridis has primarily been used to further our understanding of C4 photosynthesis, but is also an ideal system for the study of biomass crops, which are almost exclusively C4 panicoid grasses. Biogenesis of stem tissue, its cell wall composition, and soluble sugar content are important determinants of bioenergy crop yields. Here we show that a developing S. viridis internode is a valuable experimental system for gene discovery in relation to these important bioenergy feedstock traits.
The rate of maximal stem biomass accumulation in S. viridis A10 under long day growth was at the half-head emergence developmental stage. At this stage of development, internode 5 (of 7) was found to be rapidly expanding with an active meristem, a zone of cell expansion (primary cell walls), a transitional zone where cell expansion ceased and secondary cell wall deposition was initiated, and a mature zone that was actively accumulating soluble sugars. A simple method for identifying these zones was established allowing rapid dissection and snap-freezing for RNAseq analysis. A transcriptome profile was generated for each zone showing a transition from cell division and nucleic acid synthesis/processing in the meristem, to metabolism, energy synthesis, and primary cell wall synthesis in the cell expansion zone, to secondary cell wall synthesis in the transitional zone, to sugar transport, and photosynthesis in the mature zone.
The identification of these zones has provided a valuable experimental system for investigating key bioenergy traits, including meristematic activity, cell wall biosynthesis, and soluble sugar accumulation, in a C4 panicoid grass that has genetic resources, a short life cycle, and small stature allowing controlled experimental conditions in growth cabinets. Here we have presented a comprehensive map of gene expression and metabolites in this experimental system to facilitate gene discovery and controlled hypothesis testing for bioenergy research in S. viridis.
KeywordsSetaria viridis RNAseq Stem Bioenergy Cell wall Sugar C4 grass
cell expansion zone
principle component analysis
fragments per kilobase per million reads
sugar efflux proteins
cell wall invertase
tonoplast monosaccharide transporters
plasmodesmatal localised proteins
cellulose synthase-like proteins
Setaria viridis, the wild relative of foxtail millet (Setaria italica), has recently been adopted as a model species for the study of C4 panicoid grasses by the photosynthesis research community [1, 2, 3, 4, 5, 6]. The support of the research community for this model is evidenced by the first Setaria conference held in Beijing in 2014 . Reported uses of Setaria viridis to date have focused on the collection of natural diversity panels [8, 9], the development of mapping populations , transformation [11, 12, 13, 14] and crossing  techniques, and generation of molecular resources such as transformation vectors  and notably a genome sequence . Most research using these resources has been directed towards the study of C4 photosynthesis and associated leaf development and cellular differentiation ; however, S. viridis is also a valuable model species for the study of biomass crops, which are almost exclusively C4 panicoid grasses (e.g. giant miscanthus (Miscanthus x giganteus), switchgrass (Panicum virgatum), sugarcane (Saccharum officinarum), maize (Zea mays), and sorghum (Sorghum bicolor)) sharing a highly similar lineage to Setaria. Whilst photosynthesis is an important determinant of crop biomass yield, knowledge of the factors controlling biogenesis of stem sink tissue, its cell wall composition, and soluble sugar content are crucial for the improvement of forage and bioenergy crop quality and yields.
The main agricultural products of forage and bioenergy crops are soluble sugars and the cell wall fractions of culms, which are used as a source of feed or fuel. To maximise yields, larger culm volumes with high sugar content and easily hydrolysed cell wall polymers are required. The cell wall composition of S. viridis has been shown to be consistent with that of major bioenergy crops with equivalent conversion rates of cellulose to fermentable sugars . No study of soluble sugar levels in S. viridis culms has been published; however, Brix concentrations of up to ten can be achieved (Martin et al. unpublished observations), which is equivalent to that of mid-range sweet sorghum  and sugarcane  crops. Setaria viridis culms therefore possess attributes that suggest they would be a valuable C4 model system for the study of not only cell wall synthesis and stem development but also sugar accumulation in C4 panicoid grass culms.
A grass culm consists of multiple repeating phytomeric units, comprised of a node and an internode. Stalks develop and elongate acropetally, whilst individual internodes develop and elongate basipetally with a sigmoidal elongation pattern . At the midway stage in its development, an internode will contain an active intercalary meristem at its base, acropetally followed by a cell expansion zone, before transitioning into mature cells towards the top. Based on early physiological studies of oat culm development  and extensive literature in sugarcane and sweet sorghums, which show the accumulation of sugar in mature internodes [23, 24, 25], the following picture emerges for a developing internode in panicoid grass species. The meristem contains actively dividing cells undergoing mitosis and cell differentiation processes, and the expansion zone contains cells that are actively expanding under turgor whilst synthesising primary cell walls and cell membranes. As the cells mature, secondary cell wall deposition terminates cell expansion and the cells realise their capacity to import and store sugar.
Here we characterise a developing S. viridis internode that contains an active intercalary meristem, rapidly expanding cells with primary cell walls, a transitional zone where secondary cell wall synthesis is initiated, followed by a mature zone where sugar import and storage are occurring. RNA sequencing of tissues from each of these developmental zones provides a framework for gene discovery. Ultimately, genetic manipulation of genes discovered here has the potential to alter sink size by affecting cell division and expansion in the meristematic and cell expansion zones, and to influence the molecular composition of cell wall polymers and soluble sugars by affecting metabolic pathways in the transitional and maturation zones. Whilst there have been other transcript studies conducted in grass stems at various stages of development, including young and mature maize internodes , and in bamboo , this is the first study to characterise and utilise the developmental zones within an elongating internode, and the first whole RNAseq transcriptome dataset for S. viridis stem tissue. This experimental system and transcriptomic resource in the C4 model species, S. viridis, will facilitate gene discovery with respect to grass culm development and allow controlled experimental exploration of traits relevant to bioenergy crops such as culm sink size, cell wall composition, and soluble sugar content. The genetic and physiological similarities between Setaria viridis and key bioenergy crops will increase the translatability of results into forage and bioenergy crop improvement programmes.
To define the meristematic, cell expansion, transitional, and mature zones of internode five, nuclei density and cell size were examined using fluorescence microscopy. It was also essential to develop a method for visually identifying these zones of internode five so that harvest for RNAseq analysis could be achieved rapidly, without any need for microscopy, and therefore without excessive tissue damage.
Using brightfield microscopy, it was evident that there were many different cell types within these zones. The cell expansion zone contained immature vascular bundles with protoxylem and very little cell wall thickenings in any cell types (Fig. 2e). The transition zone contained fully developed vascular bundles with fully developed xylem, but only modest cell wall thickenings (Fig. 2f). The mature zone contained fully developed vascular bundles with fully developed xylem and more cell wall thickening than the transitional zone (Fig. 2g). Based on evidence from these images, the dissected zones contained a variety of cell types, but were sufficiently enriched in expanding, transitioning, and mature tissues to reveal differences in metabolite levels and gene expression between each developmental stage.
The lignin content of cell walls was also measured in the cell expansion, transitional, and mature internode zones as an indicator of the presence of primary or secondary cell walls (Fig. 3). Very low levels of lignin were observed in the cell expansion zone; however, the cell walls rapidly became lignified in the transitional zone, and continued to accumulate lignin up to the mature zone (Fig. 3). This suggested that the cell expansion zone contained primary cell walls, whereas the transitional zone was rapidly synthesising secondary cell walls, and that this synthesis was maintained, up to higher levels in the mature zone where it has, likely, completed lignification.
Comparing log2 fold-change expression of each gene to that obtained from a ‘whole plant’ background transcriptome , lists of genes that were up-regulated (>1 log2 fold) in each zone were generated and displayed as a Venn diagram (Fig. 4b). This also revealed a dichotomy between the two younger meristematic and cell expansion zones, and the two older transitional and mature zones. Thousand eight hundred and sixty genes were up-regulated in common in MsZ/CEZ and 983 in TZ/MZ. In comparison, only 52 MsZ/TZ, 157 MsZ/MZ, 213 CEZ/TZ, and 12 CEZ/MZ genes were up-regulated in common (Fig. 4b). The number of up-regulated genes and the number expressed above a threshold of one fragments per kilobase per million reads (FPKM) also showed dichotomy with more being found in younger than older tissues (Fig. 4c). This suggests that greater biological complexity is required for meristematic activity and cell expansion than for the established biological processes of mature tissues including secondary cell wall synthesis and carbon storage.
Using criteria of gene expression above a level of 80 FPKM (chosen by visually examining read coverage of genes with ~80 FPKM), and a log2 fold change >2.5 against the whole plant (WP) background transcriptome, 418 ‘stem-enriched’, 13 ‘MsZ-enriched’, 8 ‘CEZ-enriched’, 84 ‘TZ-enriched’, and 45 ‘MZ-enriched’ genes were identified which can be used to identify promoter sequences for the construction of tissue-specific, and likely cell-specific, expression vectors (‘enriched’ genes are marked in Additional file 2).
Setaria viridis is becoming more widely accepted as a C4 grass model species, especially within the photosynthesis research community. Here we have presented the first transcriptomic analysis of this model system in tissue relevant to bioenergy research, at a gene discovery level. We have identified and shown that a developing S. viridis internode contains (i) an active meristematic zone that utilises starch and likely symplasmically imported sucrose and malate as a carbon source to drive metabolism, energy production, and synthesis of DNA, RNA, and protein, (ii) a zone of cell expansion with predominantly non-lignified primary cell walls which also utilises starch and symplasmically imported sugars, in the form of hexoses to produce energy, driving cell expansion, (iii) a transitional zone where cell expansion ceases, secondary cell wall synthesis is initiated and sugar import switches to a, likely, apoplasmic step, and (iv) a mature zone containing thickened, lignified cell walls, that is actively accumulating and storing carbon in the form of sucrose and malate via the expression of energy-dependent sugar transport genes, suggesting a, likely, apoplasmic step during the unloading of photoassimilate into this tissue.
Dye feeding experiments in sugarcane  and sorghum  have shown that 5(6)-carboxyfluorescein (CF) moves symplasmically from vascular phloem to storage parenchyma cells in mature stem sink tissues. Interestingly, high expression of energy-dependent sugar transport genes within mature internodes has also been observed, in sorghum ; however, the protein cellular localisation, and therefore precise role in sugar accumulation within grass stems has not yet been elucidated. The strong expression of SWEET, SUT, HT, PLT, and CWI genes in mature sink tissue of the S. viridis developing internode provides evidence that an apoplasmic step may exist in Setaria. Localisation and characterisation of these sugar transport proteins, however, will determine the cellular localisation of a possible apoplasmic unloading step, which may be vascular in origin or located on storage parenchyma cells to facilitate unloading of sugar into the apoplasm for storage and regulation of turgor. Whilst the role of these transporters in phloem unloading and assimilate storage in maturing internodes is yet to be investigated, this experimental system has provided gene candidates and a biological system for protein localisation and phenotyping of gene knockdown lines which will further unravel this biological process that is integral to controlling soluble sugar levels in C4 panicoid grasses.
Similarly, the clear transition from primary cell wall synthesis to secondary cell wall synthesis in this system, coupled with gene expression data, has uncovered numerous S. viridis gene candidates which can now be manipulated and studied under controlled conditions to further our understanding of cell wall biosynthesis in C4 grass stems. Cellulose synthase-like (CSL) families A, C, D, F, and H were all switched on in the meristematic and cell expansion zones of the internode, implicating a potential role in cell division and/or primary cell wall synthesis of hemicelluloses. As an example of gene discovery in this system, we will examine the proteins encoded by the CSLF and CSLH gene families. Homologues of CSLF and CSLH have been shown to produce mixed linkage glucans (MLGs) in primary cell walls of barley [38, 39] during wall expansion, and in secondary cell walls of rice stems . The dataset presented here has identified a CSLF and a CSLH gene expressed in primary cell wall tissues, and a CSLF gene expressed in mature tissues (Fig. 6) differentiating transcriptional control over MLGs in primary and secondary cell walls of S. viridis. Characterisation in this biological system by protein localisation, promoter reporter constructs, and genetic knockdown using available S. viridis transformation techniques [12, 13] is now possible to further our understanding of the role of each of these genes in MLG deposition. Similarly, a number of gene isoforms can now be identified within this dataset for the manipulation and characterisation of lignin precursor synthesis, lignin polymerisation, cellulose synthesis, and hemicellulose synthesis, examining genes associated with both primary and secondary cell wall synthesis (Fig. 6). Expression of transcription factors in this experimental system was not characterised here but would also be of great interest when manipulating cell wall deposition and composition in this experimental C4 panicoid grass system.
The developing internode characterised here is a valuable experimental system for gene discovery related to a variety of fundamental biological processes, especially for traits important in determining bioenergy crop yields. These include meristematic control of sink size, biosynthesis of cell walls, and accumulation of soluble sugars. Coupling this resource with genetic transformation techniques in S. viridis [12, 13, 14], generating controlled experimental data for the genetic manipulation of sink size, cell wall composition, and soluble sugar content in a C4 grass model system is now possible.
Plant growth conditions
Setaria viridis A10 was grown in 2 L of 7:3:5:5 top soil:sand:perlite:crushed clay with 1 g/L of Plantacote depot 4M© (14:9:15 NPK) inside a growth cabinet with 16/8 h, 28/20C, and 55/70 %rH day/nights. Plants were illuminated with ~600 μmol s−1 m−2 PAR from fluorescent lamps during light hours. After germination, plants were thinned to leave one plant per pot and were given 150 mL of water (with 1.5 mL/L of Previcur® on the first watering) approximately 3 h into the light cycle every day.
Identification of a developing internode
Plants were harvested at seven developmental stages. These were defined as the 6-leaf stage (emerging but still straight), 9-leaf (emerging but still straight), booting, half emerged head, anthesis (3/4 visible pollen), milky dough, and hard dough. Dry weights of whole plants and internodes were measured by freeze drying for ~5 days to determine biomass. Plant heights and internode lengths were also measured. Whole stems were frozen slowly at −20 °C and thawed before centrifuging through glass wool filters to obtain the soluble fraction of the stem. Sucrose was measured in the soluble fraction by FTIR spectroscopy as previously described .
Histological determination of developmental zones within the internode
The fifth internode from the base of the main stalk harvested at the half emerged stage was photographed, dissected, and fixed in 4 % (w/v) paraformaldehyde/1.5 % (w/v) glutaraldehyde. Fixed tissue was halved longitudinally, embedded in 6 % agarose and sectioned longitudinally on a vibratome along the entire length of the internode at a thickness of 50 μm. These sections were stained with 10 μg/mL DAPI and viewed under UV illumination on a Zeiss Axiophot microscope (Carl Zeiss Pty. Ltd., Oberkochen, Germany, http://www.zeiss.com). Nuclei, and therefore cells, were counted along the length of the internode using the ITCN plugin for imageJ (http://imagej.nih.gov/ij/plugins/itcn.html). Nuclei density and cell size were calculated in a sliding window by dividing the number of each within the window by the width of the window at 5-μm interval. Cell size and nuclei density were immediately matched with defining features of the unsectioned half of the internode. Other internodes were quartered transversely, dehydrated through an ethanol series, and infiltrated with Technovit© (Heraeus Kulzer, Hanau, Germany, http://www.heraeus-kulzer.com). Technovit© blocks were polymerised and 4-μm transverse sections were cut on a microtome. Sections were stained with 0.05 % Toluidine blue and counterstained with Lugol’s iodine before viewing under brightfield illumination.
Harvest of a developing internode
Eighty plants were harvested at the half emerged head stage (see “Results” section for details) from two crops grown under the same conditions (40 plants per crop) between 8 and 11 h into the light cycle. Harvest was staggered as plants reached the half emerged stage from 25 to 28 days after sowing (DAS). Plants were photographed; height and fresh weight were measured to ensure uniformity, and the fifth internode was dissected immediately after removal from the growth cabinet. The leaf sheath was removed from the fifth internode, and the intercalary meristem, cell expansion, transition, and mature zones were dissected and snap frozen in liquid N2. Since each dissected segment was only 5–15 mg, tissue was pooled from ten plants for each replicate to generate four biological replicate pools for each developmental zone.
Acetyl bromide lignin
Twenty mg of fresh, ground tissue was weighed and washed sequentially with water, ethanol, and acetone before being freeze dried overnight. Lignin was solubilised by incubating in a thermomixer at 55 °C and 600 rpm for 150 min in 25 % (v/v) acetyl bromide prepared with glacial acetic acid. After cooling, acetic acid: 2 M NaOH mixture (50:9 v/v) and 0.5 M hydroxylamine chlorhydrate were added and the absorbance was read at 280 nm. Acetyl bromide lignin content was calculated using an extinction coefficient of 20.48 that was previously determined for corn stover .
Soluble sugars and malate were measured enzymatically in ethanolic extracts from 20 mg fresh weight of frozen, ground tissue powder, and starch was measured enzymatically in the ethanol-insoluble residue, as previously described .
RNA extraction, library preparation, and sequencing
Samples were ground to a powder at −60 °C using a cryogenic grinding robot (Labman Automation, Middlesborough, UK; http://www.labman.co.uk). RNA was extracted from aliquots (80–100 mg FW) of frozen tissue powder using a standard TRIzol© (Life technologies, Carlsbad, USA, http://www.lifetechnologies.com) extraction method and precipitated with EtOH. DNase treatment was performed using Turbo DNA-free™ kit (Life technologies, Carlsbad, USA, http://www.lifetechnologies.com) as per the manufacturer’s instruction. RNA quality was assessed by analysis with an RNA 6000 Nano kit and Bioanalyzer 2100 (Agilent Technologies; Santa Clara, CA, USA; http://www.agilent.com). RIN scores of above eight were considered acceptable. RNAseq libraries were generated with polyA enrichment and rRNA depletion using NEBNext® Ultra™ Directional RNA Library Prep Kit (NEB, Ipswich, USA, http://www.neb.com) for Illumina® and were sequenced on an Illumina® HiSeq 2500 at the Max Planck Genome Centre, Cologne, Germany.
Illumina 100 bp paired-end reads from four biological replicates of the four internode zones were imported into CLC genomics workbench 8 (CLCbio, Aarhus, Denmark, http://www.clcbio.com) for adapter removal, trimming, mapping, and read counting. A single-end Illumina read library, generated from a mixture of leaf, stem, node, crown, root, spikelet, floret, and seed tissue across three developmental stages (seed germination, vegetative growth, and reproduction), was downloaded from the NCBI short read archive (SRA784127), imported into CLC genomics workbench 8, and analysed in parallel with the internode data. This ‘whole plant’ sample was used as an indication of background expression for the assessment of stem- and zone-specific gene expression.
Adapter sequences were trimmed from all reads. Low-quality reads were trimmed using a modified Mott trimming algorithm within CLC genomics 8. Reads with a quality score limit below 0.05 and regions containing more than two base ambiguities were trimmed. Reads were then mapped to the S. italica genome version 2.1 with gene annotations obtained from Phytozome 10.1 on the 29th April, 2015 , since an annotated Setaria viridis transcriptome and genome were not publically available. Mapping was performed using the principles of Mortazavi et al.  using a mismatch cost of 2, insertion cost of 3, deletion cost of 3, a length fraction of 0.8, and a similarity fraction of 0.8. Paired read distances were set at 180–660 bp, based on Bioanalyser runs of the RNA libraries, and the maximum number of hits for a read was set at 10. Fragments per kilobase per million (FPKM) was calculated for each gene, and EDGE test  and ANOVA false discovery rates were calculated. Log2 fold change between internode regions was calculated against the average of the four zones for each gene using x2 transformed data. Mapman bins were assigned to genes using the Mapman Phytozome v9.0 mapping file for S. italica downloaded on the 12th May, 2015. Genes encoding sugar transporters and cell wall synthesis related enzymes/proteins were manually annotated by BLAST against maize, rice, and sorghum proteome sequences and Mapman bins were assigned accordingly. A file containing FPKM, log2 fold change, Mapman bin numbers, and gene annotations can be found in Additional file 2. This file was used, along with our updated Mapman mapping file (Additional file 3) to generate Mapman visualisations of the RNAseq dataset.
Principal component analysis was performed in ‘The Unscrambler® X 10.2’ (CAMO) with standard deviation-weighted FPKM values using the NIPALS algorithm and full cross validation . Venn diagrams were generated using ‘Venny 2.0’ . Gene categories of interest were selected using the Mapman binning system in Additional file 3 and average FPKM was calculated for each category.
APM, WMP, CPLG, and RTF conceived the study. APM designed and conducted all experiments, analysed all data, produced all figures, and drafted the manuscript. WMP contributed to data analysis, drafting of the manuscript, and production of figures. CB performed some preliminary RNAseq data analysis and reviewed the manuscript. CA optimised Setaria growth conditions and reviewed the manuscript. JEL provided intellectual input and resources for Setaria growth, RNA sequencing, and metabolite measurements. RTF, JEL, and CPLG revised the manuscript. All authors read and approved the final manuscript.
The authors would like to thank Marc Lohse for initial discussion about sequencing strategies, Armin Schlereth for assistance with preparation of samples for RNA sequencing, Eugenia Maximova for assistance with histology, Gretel Linich for retrieving datasets, Carlos Figueroa for assistance with starch measurements, Beatrice Encke and Regina Feil for assistance with metabolite measurements, and Grégory Mouille, Sylvie Citerne and Stéphanie Boutet for assistance in the measurement of cell wall components. The authors acknowledge the use of the Australian Plant Phenomics Facility, for plant growth and sample preparation in this work.
The authors declare that they have no competing interests.
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