Fig. 4 | Orphanet Journal of Rare Diseases

Fig. 4

From: Protein signature of human skin fibroblasts allows the study of the molecular etiology of rare neurological diseases

Fig. 4

CARS microscopic findings in the muscle biopsy of an Allgrove syndrome patient: F-CARS images are shown in red and E-SHG images in green. Most CARS/SHG images were taken at 2932 cm−1, representing protein distribution. The remaining images show lipid distribution at 2841 cm−1 (C, L, M, O). “Empty” vacuoles with and without rims: a Without rim (scale bar 2.0 µm). b With protein rim (marked with arrow; 1.7 µm). c With lipid rim (6.3 µm). d Fuzzy border (external dotted line marks the assumed boundary; inner dotted line surrounds the "empty" centre; 1.8 µm). e With E-SHG rim (1.45 µm). Filled vacuoles with and without rims: f Protein filled without rim (1.4 µm). g ‘E-SHG filled’ without rim (dotted line marks border; 1.95 µm). h Protein filled with ‘E-SHG rim’ (dotted line marks border, arrows mark rim; 1.1 µm). “Empty” and filled amorphous vacuoles with rims: i Empty with protein rim (arrows mark rim; 3.0 µm). j Empty with lipid rim (arrows mark rim; 4.8 µm). k Protein filled with protein rim (arrows mark rim; 2.25 µm). Lipid accumulations: l Cloud-like accumulation (7.1 µm). m Dot-like accumulations (marked with arrows; 7.7 µm). E-SHG accumulation: n Cloud-like accumulation (marked with arrows; 14 µm). Degeneration: o Fatty degeneration (marked with asterisks; 39 µm). p Fibrous degeneration (dotted line shows the borders of a former muscle fibre, which has been replaced by connective tissue; 17 µm). Fibre splitting: q Splitting (marked with arrows; 60 µm). F-CARS spectra: The spectra were obtained from measurements of the corresponding feature findings. Black spectra were taken from the inside of vacuoles or lipid accumulations (L, M, O). Red spectra were taken from vacuole rims. The cell background (blue) was taken from inconspicuous areas near the features. The standard deviation is shown in grey. The vertical dotted lines highlight the wave numbers 2847 cm−1, 2868 cm−1 and 2921 cm−1

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